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Literature summary extracted from

  • Sondhi, S.; Sharma, P.; Saini, S.; Puri, N.; Gupta, N.
    Purification and characterization of an extracellular, thermo-alkali-stable, metal tolerant laccase from Bacillus tequilensis SN4 (2014), PLoS ONE, 9, e96951 .
    View publication on PubMedView publication on EuropePMC

General Stability

EC Number General Stability Organism
1.10.3.2 the enzyme is stable in the presence of cationic and anionic detergents. In the presence of non-ionic detergents, the enzyme is stable at lower concentrations Bacillus tequilensis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 2-mercaptoethanol 1 mM, complete inactivation Bacillus tequilensis
1.10.3.2 cysteine 5 mM, 92% loss of activity Bacillus tequilensis
1.10.3.2 Diethylenetriaminepentaacetic acid 10 mM, 76% loss of activity Bacillus tequilensis
1.10.3.2 dithiothreitol 5 mM, complete inactivation. 1 mM, 80% loss of activity Bacillus tequilensis
1.10.3.2 EDTA 10 mM, 77% loss of activity Bacillus tequilensis
1.10.3.2 Fe2+ 5 mM, 59% loss of activity Bacillus tequilensis
1.10.3.2 Hg2+ 1.0 mM, 48% loss of activity Bacillus tequilensis
1.10.3.2 Li+ 5 mM, 22% loss of activity Bacillus tequilensis
1.10.3.2 Mg2+ 5 mM, 23% loss of activity Bacillus tequilensis
1.10.3.2 Mn2+ 5 mM, 21% loss of activity Bacillus tequilensis
1.10.3.2 NaN3 10 mM, 68% loss of activity Bacillus tequilensis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.84
-
2,6-dimethoxyphenol pH 8.0, 85°C Bacillus tequilensis
1.10.3.2 1.4
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 8.0, 85°C Bacillus tequilensis
1.10.3.2 3.26
-
guaiacol pH 8.0, 85°C Bacillus tequilensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.10.3.2 extracellular
-
Bacillus tequilensis
-
-

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Co2+ 5.0 mM, increased activity to 150% Bacillus tequilensis
1.10.3.2 Cu2+ 5.0 mM, increased activity to 126% Bacillus tequilensis

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Bacillus tequilensis
-
-
-
1.10.3.2 Bacillus tequilensis SN4
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
Bacillus tequilensis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
Bacillus tequilensis ?
-
?
1.10.3.2 2,6-dimethoxyphenol + O2
-
Bacillus tequilensis ?
-
?
1.10.3.2 alpha-naphthol + O2
-
Bacillus tequilensis ?
-
?
1.10.3.2 catechol + O2
-
Bacillus tequilensis ?
-
?
1.10.3.2 catechol + O2
-
Bacillus tequilensis SN4 ?
-
?
1.10.3.2 guaiacol + O2
-
Bacillus tequilensis ?
-
?
1.10.3.2 L-DOPA + O2
-
Bacillus tequilensis ?
-
?
1.10.3.2 additional information poorly oxidizes m-phenols (resorcinol) and shows no activity towards tyrosine Bacillus tequilensis ?
-
?
1.10.3.2 additional information poorly oxidizes m-phenols (resorcinol) and shows no activity towards tyrosine Bacillus tequilensis SN4 ?
-
?
1.10.3.2 p-phenylenediamine + O2
-
Bacillus tequilensis ?
-
?
1.10.3.2 p-phenylenediamine + O2
-
Bacillus tequilensis SN4 ?
-
?
1.10.3.2 pyrogallol + O2
-
Bacillus tequilensis ?
-
?
1.10.3.2 pyrogallol + O2
-
Bacillus tequilensis SN4 ?
-
?
1.10.3.2 syringaldazine + O2
-
Bacillus tequilensis ?
-
?
1.10.3.2 syringaldazine + O2
-
Bacillus tequilensis SN4 ?
-
?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 monomer 1 * 32000, SDS-PAGE Bacillus tequilensis

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 SN4LAC
-
Bacillus tequilensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 85
-
-
Bacillus tequilensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 65 100 65°C: about 50% of maximal activity, 100°C: about 50% of maximal activity Bacillus tequilensis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 65
-
24 h, completely stable Bacillus tequilensis
1.10.3.2 75
-
half-life: 4 h Bacillus tequilensis
1.10.3.2 80
-
half-life: 3 h Bacillus tequilensis
1.10.3.2 85
-
half-life: 1 h Bacillus tequilensis

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 62.96
-
guaiacol pH 8.0, 85°C Bacillus tequilensis
1.10.3.2 67.04
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 8.0, 85°C Bacillus tequilensis
1.10.3.2 73.15
-
2,6-dimethoxyphenol pH 8.0, 85°C Bacillus tequilensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5.5
-
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus tequilensis
1.10.3.2 6.5
-
substrate: syringaldazine Bacillus tequilensis
1.10.3.2 8
-
substrate: 2,6-dimethoxyphenol Bacillus tequilensis
1.10.3.2 8
-
substrate: guaiacol Bacillus tequilensis

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 9
-
24 h, the enzyme retains 75% of its activity Bacillus tequilensis

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 19.34
-
guaiacol pH 8.0, 85°C Bacillus tequilensis
1.10.3.2 47.82
-
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 8.0, 85°C Bacillus tequilensis
1.10.3.2 87.05
-
2,6-dimethoxyphenol pH 8.0, 85°C Bacillus tequilensis