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Literature summary extracted from

  • Gu, C.; Zheng, F.; Long, L.; Wang, J.; Ding, S.
    Engineering the expression and characterization of two novel laccase isoenzymes from Coprinus comatus in Pichia pastoris by fusing an additional ten amino acids tag at N-terminus (2014), PLoS ONE, 9, e93912 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 expression in in Pichia pastoris by fusing an additional ten amino acids (Thr-Pro-Phe-Pro-Pro-Phe-Asn-Thr-Asn-Ser) tag at N-terminus Coprinus comatus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 dithiothreitol 0.1 mM, complete inactivation; 0.1 mM, complete inactivation Coprinus comatus
1.10.3.2 EDTA 50 mM, 70% loss of activity, laccase 3; 50 mM, 80% loss of activity, laccase 4 Coprinus comatus
1.10.3.2 additional information isoenzyme Lac4 is resistant to SDS, and retains 43.08% activity in the presence of 100 mM SDS; isoenzymes Lac3 is resistant to SDS, and retains 31.86% activity in the presence of 100 mM SDS Coprinus comatus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.087
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 0.136
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 0.204
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.10.3.2 0.205
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.10.3.2 0.455
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.10.3.2 1.114
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 1.374
-
 2,6-dimethylphenol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 7.131
-
 2,6-dimethylphenol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 additional information metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzyme Lac4 Coprinus comatus
1.10.3.2 additional information metal ions such as Ca2+, Mg2+, Co2+, Zn2+, Mn2+ and Cu2+ have slight or even no effect on the activities of laccase isoenzymes Lac3 and Lac4 Coprinus comatus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Coprinus comatus X5JA14 isoenzyme laccase 3
-
1.10.3.2 Coprinus comatus X5JAK9 isoenzyme laccase 4
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein when expressed in Pichia pastoris, the recombinant laccase is hyperglycosylated Coprinus comatus

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 recombinant enzyme Coprinus comatus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 2,6-dimethylphenol + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Coprinus comatus ?
-
 ?
1.10.3.2 additional information Lac3 exhibits higher decolorization efficiency than Lac4 for eleven out of thirteen different dyes. Mild synergistic decolorization by the laccases isoenzymes Lac 3 and Lac4 is observed for triphenylmethane dyes but not for anthraquinone and azo dyes Coprinus comatus ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Coprinus comatus ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 70000, isoenzyme Lac4 Coprinus comatus
1.10.3.2 ? x * 75000, isoenzyme Lac3 Coprinus comatus

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 LAC3
-
 Coprinus comatus
1.10.3.2 Lac4
-
 Coprinus comatus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
 substrate: guaiacol, isoenzyme Lac3 Coprinus comatus
1.10.3.2 60
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac4 Coprinus comatus
1.10.3.2 60
-
 substrate: 2,6-dimethylyphenol, isoenzyme Lac3 Coprinus comatus
1.10.3.2 60
-
 substrate: guaiacol, isoenzyme Lac4 Coprinus comatus
1.10.3.2 60
-
 substrate: syringaldazine, isoenzyme Lac3 Coprinus comatus
1.10.3.2 65
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac3 Coprinus comatus
1.10.3.2 65
-
 substrate: 2,6-dimethylyphenol, isoenzyme Lac4 Coprinus comatus
1.10.3.2 65
-
 substrate: syringaldazine, isoenzyme Lac4 Coprinus comatus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50
-
 recombinant laccase is stable at temperatures below 50°C Coprinus comatus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 0.196
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.10.3.2 0.307
-
 2,6-dimethylphenol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 0.418
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 1.467
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.10.3.2 1.468
-
 2,6-dimethylphenol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.10.3.2 3.485
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 31.96
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 44.71
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac3 Coprinus comatus
1.10.3.2 3
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), isoenzyme Lac4 Coprinus comatus
1.10.3.2 5
-
 substrate: 2,6-dimethylyphenol, isoenzyme Lac3 Coprinus comatus
1.10.3.2 5
-
 substrate: guaiacol, isoenzyme Lac3 Coprinus comatus
1.10.3.2 5.5
-
 substrate: syringaldazine, isoenzyme Lac3 Coprinus comatus
1.10.3.2 5.5
-
 substrate: syringaldazine, isoenzyme Lac4 Coprinus comatus
1.10.3.2 6
-
 substrate: 2,6-dimethylyphenol, isoenzyme Lac4 Coprinus comatus
1.10.3.2 6
-
 substrate: guaiacol, isoenzyme Lac4 Coprinus comatus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Coprinus comatus isoenzyme Lac3
-
 5.16
1.10.3.2 Coprinus comatus isoenzyme Lac4
-
 5.8

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 0.375
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 0.503
-
 guaiacol pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.10.3.2 7.167
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.10.3.2 25.625
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus
1.10.3.2 219.6
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac4 Coprinus comatus
1.10.3.2 367.36
-
 syringaldazine pH 4.5, 40°C, isoenzyme Lac3 Coprinus comatus