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Literature summary extracted from

  • Yamamoto, R.; Yamamoto, Y.; Imai, S.; Fukutomi, R.; Ozawa, Y.; Abe, M.; Matuo, Y.; Saito, K.
    Effects of various phytochemicals on indoleamine 2,3-dioxygenase 1 activity galanal is a novel, competitive inhibitor of the enzyme (2014), PLoS ONE, 9, e88789 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.13.11.52 gene IDO1, semi-quantitative RT-PCR and quantitative real-time RT-PCR enzyme expression analysis in lipopolysaccgaride-stimulated THP-1 cells, recombinant expression of N-terminally His-tagged enzyme in Escherichia coli Homo sapiens

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.13.11.52 1-methyl tryptophan a tryptophan analogue. Galanal does not decrease the IkappaB-alpha expression in LPS-stimulated THP-1 cells Homo sapiens
1.13.11.52 galanal competitive inhibitor, IC50 value of 45 nM in the cell-based assay. galanal interfered with the transcriptional function of the nuclear factor-kappaB and the interferon-gamma signaling pathway. These effects of galanal are important for immune response. The inhibitory effect of galanal on IDO1 activity is stronger than that of 1-methyl tryptophan, a tryptophan analogue Homo sapiens
1.13.11.52 additional information suppressive effect of plant extracts or phytochemicals on IDO1 induction and activity. The methanol extracts of Myoga flower buds, which are traditional Japanese foods, and labdane-type diterpene galanal derived from Myoga flowers significantly suppress IDO1 activity Homo sapiens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.13.11.52 additional information
-
 additional information Michaelis-Menten kinetics Homo sapiens
1.13.11.52 0.0526
-
 L-tryptophan pH 6.5, 37°C, recombinant His-tagged enzyme Homo sapiens
1.13.11.52 0.0745
-
 L-tryptophan pH 6.5, 37°C, recombinant His-tagged enzyme in presence of inhibitor galanal Homo sapiens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.13.11.52 D-tryptophan + O2 Homo sapiens
-
 N-formyl-D-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2 Homo sapiens
-
 N-formyl-L-kynurenine
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.13.11.52 Homo sapiens P14902
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.13.11.52 THP-1 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.13.11.52 D-tryptophan + O2
-
 Homo sapiens N-formyl-D-kynurenine
-
 ?
1.13.11.52 L-tryptophan + O2
-
 Homo sapiens N-formyl-L-kynurenine
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.13.11.52 IDO1
-
 Homo sapiens
1.13.11.52 indoleamine 2,3-dioxygenase 1
-
 Homo sapiens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.13.11.52 37
-
 assay at Homo sapiens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.13.11.52 6.5
-
 assay at Homo sapiens

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.13.11.52 0.0077
-
 pH 6.5, 37°C, recombinant His-tagged enzyme Homo sapiens galanal

Expression

EC Number Organism Comment Expression
1.13.11.52 Homo sapiens the methanol extracts of Myoga flower buds, which are traditional Japanese foods, and labdane-type diterpene galanal derived from Myoga flowers significantly suppress IDO1 activity down
1.13.11.52 Homo sapiens suppressive effect of plant extracts or phytochemicals on IDO1 induction and activity additional information

General Information

EC Number General Information Comment Organism
1.13.11.52 physiological function the enzyme catalyzes the first and rate-limiting step in the degradation of L-tryptophan, has an important immunomodulatory function. The activity of IDO1 increases in various inflammatory diseases, including tumors, autoimmune diseases, and different kinds of inflammation Homo sapiens