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Literature summary extracted from

  • Yang, J.; Lin, Q.; Ng, T.B.; Ye, X.; Lin, J.
    Purification and characterization of a novel laccase from Cerrena sp. HYB07 with dye decolorizing ability (2014), PLOS ONE, 9, e110834 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.10.3.2 degradation the enzyme is potentially useful for industrial and environmental applications such as textile finishing and wastewater treatment. It decolorizes structurally different dyes and a real textile effluent Cerrena sp.
1.10.3.2 environmental protection the enzyme is potentially useful for industrial and environmental applications such as textile finishing and wastewater treatment. It decolorizes structurally different dyes and a real textile effluent Cerrena sp.

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2
-
 Cerrena sp.

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 dithiothreitol 0.1 mM, activity is reduced to 5.9% Cerrena sp.
1.10.3.2 Fe2+ 10 mM, 94% loss of activity Cerrena sp.
1.10.3.2 kojic acid 12.5 mM, about 50% loss of activity Cerrena sp.
1.10.3.2 L-cysteine 0.1 mM, activity is reduced to 5.5% Cerrena sp.
1.10.3.2 Li+ 10 mM, 51% loss of activity Cerrena sp.
1.10.3.2 additional information no significant loss of activity loss at 10 mM EDTA Cerrena sp.
1.10.3.2 SDS 0.1 mM, activity is reduced to 11.4% Cerrena sp.
1.10.3.2 Sodium azide 0.1 mM, activity is reduced to 9.2% Cerrena sp.

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.0934
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH and temperature not specified in the publication Cerrena sp.
1.10.3.2 0.2
-
 2,6-dimethoxyphenol pH and temperature not specified in the publication Cerrena sp.
1.10.3.2 0.3
-
 guaiacol pH and temperature not specified in the publication Cerrena sp.
1.10.3.2 0.529
-
 catechol pH and temperature not specified in the publication Cerrena sp.

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Ca2+ slightly stimulatory Cerrena sp.
1.10.3.2 K+ slightly stimulatory Cerrena sp.
1.10.3.2 Mg2+ slightly stimulatory Cerrena sp.
1.10.3.2 Mn2+ slightly stimulatory Cerrena sp.
1.10.3.2 Na+ slightly stimulatory Cerrena sp.
1.10.3.2 Zn2+ slightly stimulatory Cerrena sp.

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.10.3.2 58600
-
-
 Cerrena sp.

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 acetonitrile 25% (v/v), 4 h, 25°C, no loss of activity Cerrena sp.
1.10.3.2 dimethylformamide 25% (v/v),4 h, 25°C, no loss of activity Cerrena sp.
1.10.3.2 DMSO 25% (v/v), 4 h, 25°C, no loss of activity Cerrena sp.
1.10.3.2 Ethanol 25% (v/v), 4 h, 25°C, no loss of activity Cerrena sp.
1.10.3.2 Methanol 25% (v/v), 4 h, 25°C, no loss of activity Cerrena sp.

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Cerrena sp. A0A0A0Q5C7
-
-
1.10.3.2 Cerrena sp. HYB07 A0A0A0Q5C7
-
-

Posttranslational Modification

EC Number Posttranslational Modification Comment Organism
1.10.3.2 glycoprotein 7.2% carbohydrate Cerrena sp.

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Cerrena sp.

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.10.3.2 mycelium
-
 Cerrena sp.
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 1.95
-
 pH and temperature not specified in the publication Cerrena sp.

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 catalytic efficiency on 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) is 7.5 times of that on 2,6-dimethoxyphenol, 22 times of that on catechol and 65times higher than that on guaiacol Cerrena sp. ?
-
 ?
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 catalytic efficiency on 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) is 7.5 times of that on 2,6-dimethoxyphenol, 22 times of that on catechol and 65times higher than that on guaiacol Cerrena sp. HYB07 ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Cerrena sp. ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2
-
 Cerrena sp. HYB07 ?
-
 ?
1.10.3.2 catechol + O2
-
 Cerrena sp. ?
-
 ?
1.10.3.2 catechol + O2
-
 Cerrena sp. HYB07 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Cerrena sp. ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Cerrena sp. HYB07 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 58600, SDS-PAGE Cerrena sp.

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 LacA
-
 Cerrena sp.

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 45
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Cerrena sp.
1.10.3.2 45 50 substrate: guaiacol or catechol Cerrena sp.
1.10.3.2 50
-
 substrate: 2,6-dimethoxyphenol Cerrena sp.

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 25 65 the enzyme displays more than 50% of maximal activity between 25°C and 65°C against guaiacol, catechol or 2,6-dimethoxyphenol. At 65°C for 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid), the relative activity of LacA is 45% Cerrena sp.

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 50
-
 150 min, 85% of the original enzyme activity is retained Cerrena sp.
1.10.3.2 60
-
 150 min, 56% of the original enzyme activity is retained Cerrena sp.
1.10.3.2 70
-
 inactivated after 20 min Cerrena sp.
1.10.3.2 80
-
 inactivated after 2 min Cerrena sp.

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 128.1
-
 guaiacol pH and temperature not specified in the publication Cerrena sp.
1.10.3.2 626
-
 catechol pH and temperature not specified in the publication Cerrena sp.
1.10.3.2 703.7
-
 2,6-dimethoxyphenol pH and temperature not specified in the publication Cerrena sp.
1.10.3.2 2468
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH and temperature not specified in the publication Cerrena sp.

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) or 2,6-dimethoxyphenol Cerrena sp.
1.10.3.2 4
-
 substrate: guaiacol Cerrena sp.
1.10.3.2 4.5
-
 substrate: catechol Cerrena sp.

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 2
-
 activity decreases to approximately 1% at the end of 105 h incubation. t1/2: 18 h Cerrena sp.
1.10.3.2 3
-
 activity decreases to approximately 20% at the end of 105 h incubation. t1/2: 43 h Cerrena sp.
1.10.3.2 4
-
 105 h, 56% residual enzyme activity is retained Cerrena sp.
1.10.3.2 5
-
 or above, 105 h, more than 80% residual enzyme activity is retained Cerrena sp.

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 400
-
 guaiacol pH and temperature not specified in the publication Cerrena sp.
1.10.3.2 1200
-
 catechol pH and temperature not specified in the publication Cerrena sp.
1.10.3.2 2642
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH and temperature not specified in the publication Cerrena sp.
1.10.3.2 3500
-
 2,6-dimethoxyphenol pH and temperature not specified in the publication Cerrena sp.