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Literature summary extracted from
- Application of a novel alkali-tolerant thermostable DyP-type peroxidase from Saccharomonospora viridis DSM 43017 in biobleaching of eucalyptus kraft pulp (2014), PLoS ONE, 9, e110319 .
Application
| EC Number | Application | Comment | Organism |
|---|---|---|---|
| 1.11.1.19 | paper production | the enzyme has an accelerated action during the biobleaching of eucalyptus kraft pulp | Saccharomonospora viridis |
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.11.1.19 | expressed in Escherichia coli BL21 cells | Saccharomonospora viridis |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.19 | Ca2+ | 84.7% residual activity at 5 mM | Saccharomonospora viridis |  |
| 1.11.1.19 | Fe3+ | 35.9% residual activity at 5 mM | Saccharomonospora viridis | |
| 1.11.1.19 | Li+ | 87% residual activity at 5 mM | Saccharomonospora viridis | |
| 1.11.1.19 | Mn2+ | 92.3% residual activity at 5 mM | Saccharomonospora viridis | |
| 1.11.1.19 | SDS | no activity at 5 mM SDS | Saccharomonospora viridis | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.11.1.19 | 0.05887 | - |
Brilliant Green | at pH 7.0 and 70°C | Saccharomonospora viridis | |
| 1.11.1.19 | 0.1872 | - |
Reactive Yellow 2 | at pH 7.0 and 70°C | Saccharomonospora viridis | |
| 1.11.1.19 | 0.2102 | - |
Reactive Blue 19 | at pH 7.0 and 70°C | Saccharomonospora viridis | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.19 | 2-mercaptoethanol | 847.9% activity at 5 mM | Saccharomonospora viridis | |
| 1.11.1.19 | Al3+ | 127.6% activity at 5 mM | Saccharomonospora viridis | |
| 1.11.1.19 | Co2+ | 116.9% activity at 5 mM | Saccharomonospora viridis | |
| 1.11.1.19 | additional information | not influenced by Zn2+, Ba2+, Mg2+, Fe2+, and EDTA | Saccharomonospora viridis |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.11.1.19 | Saccharomonospora viridis | T1SP91 | - |
- |
| 1.11.1.19 | Saccharomonospora viridis DSM 43017 | T1SP91 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.11.1.19 | Ni-NTA agarose column chromatography and Superdex G-75 gel filtration | Saccharomonospora viridis |
Source Tissue
| EC Number | Source Tissue | Comment | Organism | Textmining |
|---|
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.19 | 0.8 | - |
enzyme from lysate supernatant, at pH 7.0 and 70°C | Saccharomonospora viridis |
| 1.11.1.19 | 17.8 | - |
after 22.3fold purification at pH 7.0 and 70°C | Saccharomonospora viridis |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.11.1.19 | 2,6-dimethoxyphenol + H2O2 + H+ | 0.5% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | 2,6-dimethoxyphenol + H2O2 + H+ | 0.5% activity compared to Brilliant Green | Saccharomonospora viridis DSM 43017 | ? | - |
? | |
| 1.11.1.19 | Azure B + H2O2 + H+ | 13.2% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | Azure B + H2O2 + H+ | 13.2% activity compared to Brilliant Green | Saccharomonospora viridis DSM 43017 | ? | - |
? | |
| 1.11.1.19 | Brilliant Green + H2O2 + H+ | 100% activity | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | Brilliant Green + H2O2 + H+ | 100% activity | Saccharomonospora viridis DSM 43017 | ? | - |
? | |
| 1.11.1.19 | Crystal Violet + H2O2 + H+ | 33.6% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | Crystal Violet + H2O2 + H+ | 33.6% activity compared to Brilliant Green | Saccharomonospora viridis DSM 43017 | ? | - |
? | |
| 1.11.1.19 | Malachite Green + H2O2 + H+ | 68.6% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | Malachite Green + H2O2 + H+ | 68.6% activity compared to Brilliant Green | Saccharomonospora viridis DSM 43017 | ? | - |
? | |
| 1.11.1.19 | Reactive Black 5 + H2O2 + H+ | 7.8% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | Reactive Blue 19 + H2O2 | 10.5% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | Reactive Green 19 + H2O2 + H+ | 10.8% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | Reactive Red 120 + H2O2 + H+ | 5.6% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | Reactive Yellow 2 + H2O2 + H+ | 39.7% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
| 1.11.1.19 | veratryl alcohol + H2O2 + H+ | 0.2% activity compared to Brilliant Green | Saccharomonospora viridis | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.11.1.19 | ? | x * 45000, SDS-PAGE | Saccharomonospora viridis |
| 1.11.1.19 | ? | x * 44500, calculated from amino acid sequence | Saccharomonospora viridis |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.11.1.19 | DyP | - |
Saccharomonospora viridis |
| 1.11.1.19 | DyP-type peroxidase | - |
Saccharomonospora viridis |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.19 | 70 | - |
- |
Saccharomonospora viridis |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.19 | 50 | 80 | the enzyme retains about 63% of its maximum activity at 50-80°C | Saccharomonospora viridis |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.19 | 70 | - |
the enzyme shows more than 60% activity after incubation at 70°C for 2 h at pH 7.0 | Saccharomonospora viridis |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.19 | 7 | - |
- |
Saccharomonospora viridis |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.19 | 4 | 9 | the enzyme shows more than 35% activity at pH 4.0-9.0 | Saccharomonospora viridis |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.11.1.19 | 5 | 10 | the enzyme shows more than 80% activity after incubation at pH 5.0-10.0 for 1 h at 37°C | Saccharomonospora viridis |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.11.1.19 | heme | - |
Saccharomonospora viridis | |
pI Value
| EC Number | Organism | Comment | pI Value Maximum | pI Value |
|---|---|---|---|---|
| 1.11.1.19 | Saccharomonospora viridis | isoelectric focusing | - |
5.2 |
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