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Literature summary extracted from

  • Pelot, K.A.; Hagelthorn, D.M.; Addison, J.B.; Zerbe, P.
    Biosynthesis of the oxygenated diterpene nezukol in the medicinal plant Isodon rubescens is catalyzed by a pair of diterpene synthases (2017), PLoS ONE, 12, e0176507 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
4.2.3.131 cloning of full-length cDNA, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, recombinant transient Agrobacterium tumefaciens-mediated coexpression of Isodon rubescens gene TPS4, encoding miltiradiene synthase, with Isodon rubescens gene TPS3, encoding (+)-copalyl diphosphate synthase in Nicotiana benthamiana results in production of miltiradiene, while coexpression with Isodon rubescens gene TPS2, encoding ent-copalyl diphosphate synthase, does not lead to product formation Isodon rubescens
4.2.3.183 Agrobacterium-mediated transient expression in Nicotiana benthamiana Isodon rubescens

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
4.2.3.131 Mg2+ required Isodon rubescens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
4.2.3.131 (+)-copalyl diphosphate Isodon rubescens
-
 miltiradiene + diphosphate
-
 ?
4.2.3.183 (+)-copalyl diphosphate + H2O Isodon rubescens
-
 nezukol + diphosphate
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
4.2.3.131 Isodon rubescens A0A1W6QDI7
-
-
4.2.3.183 Isodon rubescens A0A1W6QDI4
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
4.2.3.131 leaf high expression levels of TPS4 in leaves Isodon rubescens
-
4.2.3.183 leaf
-
 Isodon rubescens
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
4.2.3.131 (+)-copalyl diphosphate
-
 Isodon rubescens miltiradiene + diphosphate
-
 ?
4.2.3.183 (+)-copalyl diphosphate + H2O
-
 Isodon rubescens nezukol + diphosphate
-
 ?
4.2.3.183 additional information no activity of TPS2 is found when coexpressed with the ent-CPP synthase TPS5 Isodon rubescens ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
4.2.3.131 IrTPS4
-
 Isodon rubescens
4.2.3.131 TPS4
-
 Isodon rubescens
4.2.3.183 TPS2
-
 Isodon rubescens