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Literature summary extracted from

  • Stone, V.N.; Parikh, H.I.; El-rami, F.; Ge, X.; Chen, W.; Zhang, Y.; Kellogg, G.E.; Xu, P.
    Identification of small-molecule inhibitors against meso-2,6-diaminopimelate dehydrogenase from Porphyromonas gingivalis (2015), PLoS ONE, 10, e0141126 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.1.16 expressed in Escherichia coli BL21(DE3) pLysS cells Porphyromonas gingivalis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.1.16 (2E,6S)-6-aminohept-2-enedioic acid
-
 Porphyromonas gingivalis
1.4.1.16 (2S)-2-amino-6-methylideneheptanedioic acid
-
 Porphyromonas gingivalis
1.4.1.16 (3S)-3-amino-4-{[(1S)-1-carboxy-2-phenylethyl]amino}-4-oxobutanoic acid 2% inhibition at 3 mM Porphyromonas gingivalis
1.4.1.16 (5S)-5-[(2S)-2-amino-2-carboxyethyl]-4,5-dihydro-1,2-oxazole-3-carboxylic acid
-
 Porphyromonas gingivalis
1.4.1.16 2-[(3S,4S)-3-carboxy-4-propylpyrrolidin-1-yl]-6-methylpyridine-3-carboxylic acid
-
 Porphyromonas gingivalis
1.4.1.16 3-([3-[(4-methoxybenzene-1-sulfonyl)amino]quinoxalin-2-yl]amino)benzoic acid
-
 Porphyromonas gingivalis
1.4.1.16 3-([4-[(3,4-dimethylbenzene-1-sulfonyl)amino]-1-hydroxynaphthalen-2-yl]sulfanyl)propanoic acid
-
 Porphyromonas gingivalis
1.4.1.16 4-oxo-4-[4-[(quinoxalin-2-yl)sulfamoyl]anilino]butanoic acid
-
 Porphyromonas gingivalis
1.4.1.16 5-carboxy-2,2-dihydroxypentanoate 13% inhibition at 3 mM Porphyromonas gingivalis
1.4.1.16 5-[(3-carboxyphenyl)sulfamoyl]-2-hydroxybenzoic acid 6% inhibition at 3 mM Porphyromonas gingivalis
1.4.1.16 N-[3-[(benzenesulfonyl)amino]phenyl]-3,5-dimethyl-1H-pyrazole-4-sulfonamide 17% inhibition at 3 mM Porphyromonas gingivalis

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.1.16 0.06
-
 NADP+ at pH 10.5 and 25°C Porphyromonas gingivalis
1.4.1.16 0.37
-
 meso-2,6-diaminoheptanedioate at pH 10.5 and 25°C Porphyromonas gingivalis

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+ Porphyromonas gingivalis
-
 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+ Porphyromonas gingivalis W83
-
 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.1.16 Porphyromonas gingivalis Q7MW40
-
-
1.4.1.16 Porphyromonas gingivalis W83 Q7MW40
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.1.16 Ni-NTA column chromatography Porphyromonas gingivalis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Porphyromonas gingivalis L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?
1.4.1.16 meso-2,6-diaminoheptanedioate + H2O + NADP+
-
 Porphyromonas gingivalis W83 L-2-amino-6-oxoheptanedioate + NH3 + NADPH + H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.1.16 ? x * 32000, SDS-PAGE Porphyromonas gingivalis

Synonyms

EC Number Synonyms Comment Organism
1.4.1.16 DDH
-
 Porphyromonas gingivalis
1.4.1.16 meso-2,6-diaminopimelate dehydrogenase
-
 Porphyromonas gingivalis

Cofactor

EC Number Cofactor Comment Organism Structure
1.4.1.16 NADP+
-
 Porphyromonas gingivalis

IC50 Value

EC Number IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
1.4.1.16 0.157
-
 at pH 10.5 and 25°C Porphyromonas gingivalis 3-([3-[(4-methoxybenzene-1-sulfonyl)amino]quinoxalin-2-yl]amino)benzoic acid
1.4.1.16 0.31
-
 at pH 10.5 and 25°C Porphyromonas gingivalis 3-([4-[(3,4-dimethylbenzene-1-sulfonyl)amino]-1-hydroxynaphthalen-2-yl]sulfanyl)propanoic acid
1.4.1.16 0.356
-
 at pH 10.5 and 25°C Porphyromonas gingivalis 4-oxo-4-[4-[(quinoxalin-2-yl)sulfamoyl]anilino]butanoic acid
1.4.1.16 1.164
-
 at pH 10.5 and 25°C Porphyromonas gingivalis 2-[(3S,4S)-3-carboxy-4-propylpyrrolidin-1-yl]-6-methylpyridine-3-carboxylic acid