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Literature summary extracted from

  • Domitrovic, T.; Raymundo, D.P.; da Silva, T.F.; Palhano, F.L.
    Experimental evidence for a revision in the annotation of putative pyridoxamine 5-phosphate oxidases P(N/M)P from fungi (2015), PLoS ONE, 10, e0136761 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.4.3.5 expressed in Escherichia coli BL21 DE3 Rosetta cells Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2 Saccharomyces cerevisiae
-
 pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2 Saccharomyces cerevisiae BY4741
-
 pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.5 Saccharomyces cerevisiae
-
-
-
1.4.3.5 Saccharomyces cerevisiae BY4741
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.5 His Trap column chromatography and Superdex 200 gel filtration Saccharomyces cerevisiae

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.5 additional information Ylr456w and Ypr172w do not react with pyridoxine 5'-phosphate Saccharomyces cerevisiae ?
-
 ?
1.4.3.5 additional information Ylr456w and Ypr172w do not react with pyridoxine 5'-phosphate Saccharomyces cerevisiae BY4741 ?
-
 ?
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Saccharomyces cerevisiae pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?
1.4.3.5 pyridoxamine 5'-phosphate + H2O + O2
-
 Saccharomyces cerevisiae BY4741 pyridoxal 5'-phosphate + NH3 + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.5 ? x * 25000, SDS-PAGE Saccharomyces cerevisiae
1.4.3.5 ? x * 22700, enzyme form Ypr172w , calculated from amino acid sequence Saccharomyces cerevisiae
1.4.3.5 ? x * 23600, enzyme form Ylr456w, calculated from amino acid sequence Saccharomyces cerevisiae

Synonyms

EC Number Synonyms Comment Organism
1.4.3.5 PMP oxidase
-
 Saccharomyces cerevisiae
1.4.3.5 pyridoxinamine 5'-phosphate oxidase
-
 Saccharomyces cerevisiae
1.4.3.5 Ylr456w
-
 Saccharomyces cerevisiae
1.4.3.5 Ypr172w
-
 Saccharomyces cerevisiae