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Literature summary extracted from

  • Qu, X.; Pu, X.; Chen, F.; Yang, Y.; Yang, L.; Zhang, G.; Luo, Y.
    Molecular cloning, heterologous expression, and functional characterization of an NADPH-cytochrome P450 reductase gene from Camptotheca acuminata, a camptothecin-producing plant (2015), PLoS ONE, 10, e0135397 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.4 expressed in Escherichia coli BL21(DE3) cells Camptotheca acuminata

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.0187
-
 NADPH at pH 7.4 and 25°C Camptotheca acuminata
1.6.2.4 0.0514
-
 ferricytochrome c at pH 7.4 and 25°C Camptotheca acuminata
1.6.2.4 0.1193
-
 ferricyanide at pH 7.4 and 25°C Camptotheca acuminata

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Camptotheca acuminata A0A0D5BVX3
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 Ni-NTA resin column chromatography, and Superdex 200 gel filtration Camptotheca acuminata

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.6.2.4 leaf
-
 Camptotheca acuminata
-
1.6.2.4 root
-
 Camptotheca acuminata
-
1.6.2.4 stem
-
 Camptotheca acuminata
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 additional information no activity with NADH Camptotheca acuminata ?
-
 ?
1.6.2.4 NADPH + H+ + ferricyanide
-
 Camptotheca acuminata NADP+ + ferrocyanide
-
 ?
1.6.2.4 NADPH + H+ + ferricytochrome c
-
 Camptotheca acuminata NADP+ + ferrocytochrome c
-
 ?

Subunits

EC Number Subunits Comment Organism
1.6.2.4 ? x * 80000, SDS-PAGE Camptotheca acuminata

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 CPR
-
 Camptotheca acuminata
1.6.2.4 NADPH-cytochrome P450 reductase
-
 Camptotheca acuminata

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Camptotheca acuminata
1.6.2.4 FMN
-
 Camptotheca acuminata