Literature summary extracted from
Gemmecker, S.; Schaub, P.; Koschmieder, J.; Brausemann, A.; Drepper, F.; Rodriguez-Franco, M.; Ghisla, S.; Warscheid, B.; Einsle, O.; Beyer, P.
Phytoene desaturase from Oryza sativa oligomeric assembly, membrane association and preliminary 3D-analysis (2015), PLoS ONE, 10, e0131717 .
Cloned(Commentary)
EC Number |
Cloned (Comment) |
Organism |
---|
1.3.5.5 |
expression in Escherichia coli |
Oryza sativa Indica Group |
Crystallization (Commentary)
EC Number |
Crystallization (Comment) |
Organism |
---|
1.3.5.5 |
low-resolution model, homotetrameric arrangement of the protein |
Oryza sativa Indica Group |
Inhibitors
EC Number |
Inhibitors |
Comment |
Organism |
Structure |
---|
1.3.5.5 |
norflurazon |
arrests the reaction by stabilizing the intermediary FADred. An excess of the quinone acceptor relieves this blockage |
Oryza sativa Indica Group |
|
Localization
EC Number |
Localization |
Comment |
Organism |
GeneOntology No. |
Textmining |
---|
1.3.5.5 |
membrane |
associated to |
Oryza sativa Indica Group |
16020 |
- |
Molecular Weight [Da]
EC Number |
Molecular Weight [Da] |
Molecular Weight Maximum [Da] |
Comment |
Organism |
---|
1.3.5.5 |
450000 |
- |
gel filtration |
Oryza sativa Indica Group |
Organism
EC Number |
Organism |
UniProt |
Comment |
Textmining |
---|
1.3.5.5 |
Oryza sativa Indica Group |
A2XDA1 |
- |
- |
Oxidation Stability
EC Number |
Oxidation Stability |
Organism |
---|
1.3.5.5 |
reduced form of protein is stable for over 2 h in ambient oxygen atmosphere, rapid reoxidation can be achieved with excess decyl-plastoquinone |
Oryza sativa Indica Group |
Purification (Commentary)
EC Number |
Purification (Comment) |
Organism |
---|
1.3.5.5 |
recombinant protein. High-order oligomers formed in solution are the basis for an active preparation |
Oryza sativa Indica Group |
Substrates and Products (Substrate)
EC Number |
Substrates |
Comment Substrates |
Organism |
Products |
Comment (Products) |
Rev. |
Reac. |
---|
1.3.5.5 |
15-cis-phytoene + 2 decyl-plastoquinone |
- |
Oryza sativa Indica Group |
9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol |
- |
? |
|
1.3.5.5 |
additional information |
benzoquinone cosubstrates are strongly preferred over naphthoquinones |
Oryza sativa Indica Group |
? |
- |
? |
|
Subunits
EC Number |
Subunits |
Comment |
Organism |
---|
1.3.5.5 |
octamer |
8 * 56200, calculated, 8 * 56000, SDS-PAGE, recombinant protein with His-tag |
Oryza sativa Indica Group |
Synonyms
EC Number |
Synonyms |
Comment |
Organism |
---|
1.3.5.5 |
PDS1 |
- |
Oryza sativa Indica Group |
Cofactor
EC Number |
Cofactor |
Comment |
Organism |
Structure |
---|
1.3.5.5 |
FAD |
sole protein-bound redox-cofactor |
Oryza sativa Indica Group |
|