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Literature summary extracted from

  • Gemmecker, S.; Schaub, P.; Koschmieder, J.; Brausemann, A.; Drepper, F.; Rodriguez-Franco, M.; Ghisla, S.; Warscheid, B.; Einsle, O.; Beyer, P.
    Phytoene desaturase from Oryza sativa oligomeric assembly, membrane association and preliminary 3D-analysis (2015), PLoS ONE, 10, e0131717 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.5 expression in Escherichia coli Oryza sativa Indica Group

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.5.5 low-resolution model, homotetrameric arrangement of the protein Oryza sativa Indica Group

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.5.5 norflurazon arrests the reaction by stabilizing the intermediary FADred. An excess of the quinone acceptor relieves this blockage Oryza sativa Indica Group

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.3.5.5 membrane associated to Oryza sativa Indica Group 16020
-

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.3.5.5 450000
-
 gel filtration Oryza sativa Indica Group

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.5 Oryza sativa Indica Group A2XDA1
-
-

Oxidation Stability

EC Number Oxidation Stability Organism
1.3.5.5 reduced form of protein is stable for over 2 h in ambient oxygen atmosphere, rapid reoxidation can be achieved with excess decyl-plastoquinone Oryza sativa Indica Group

Purification (Commentary)

EC Number Purification (Comment) Organism
1.3.5.5 recombinant protein. High-order oligomers formed in solution are the basis for an active preparation Oryza sativa Indica Group

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.5 15-cis-phytoene + 2 decyl-plastoquinone
-
 Oryza sativa Indica Group 9,15,9'-tricis-zeta-carotene + 2 decyl-plastoquinol
-
 ?
1.3.5.5 additional information benzoquinone cosubstrates are strongly preferred over naphthoquinones Oryza sativa Indica Group ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.5.5 octamer 8 * 56200, calculated, 8 * 56000, SDS-PAGE, recombinant protein with His-tag Oryza sativa Indica Group

Synonyms

EC Number Synonyms Comment Organism
1.3.5.5 PDS1
-
 Oryza sativa Indica Group

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.5 FAD sole protein-bound redox-cofactor Oryza sativa Indica Group