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Literature summary extracted from

  • Si, W.; Wu, Z.; Wang, L.; Yang, M.; Zhao, X.
    Enzymological characterization of atm, the first laccase from Agrobacterium sp. S5-1, with the ability to enhance in vitro digestibility of maize Straw (2015), PLoS ONE, 10, e0128204 .
    View publication on PubMedView publication on EuropePMC

Application

EC Number Application Comment Organism
1.10.3.2 biofuel production potential for bioconversion of lignin rich agricultural byproducts into animal feed and cellulosic ethanol. The enzyme effectively improves in vitro digestibility of maize straw Agrobacterium sp. S5-1
1.10.3.2 food industry potential for bioconversion of lignin rich agricultural byproducts into animal feed and cellulosic ethanol. The enzyme effectively improves in vitro digestibility of maize straw Agrobacterium sp. S5-1

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 heterologously expressed in Escherichia coli Agrobacterium sp. S5-1

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 Ba2+ partially inhibits laccase activity Agrobacterium sp. S5-1
1.10.3.2 Co3+ 20 mM, weak inhibitory effect Agrobacterium sp. S5-1
1.10.3.2 dithiothreitol
-
 Agrobacterium sp. S5-1
1.10.3.2 EDTA 5 mM or 20 mM, partially inhibits activity Agrobacterium sp. S5-1
1.10.3.2 Fe2+ partially inhibits laccase activity Agrobacterium sp. S5-1
1.10.3.2 Fe3+ partially inhibits laccase activity Agrobacterium sp. S5-1
1.10.3.2 additional information Mn2+ even at 20 mM does not affect Atm activity Agrobacterium sp. S5-1
1.10.3.2 Ni2+ 20 mM, weak inhibitory effect Agrobacterium sp. S5-1
1.10.3.2 SDS
-
 Agrobacterium sp. S5-1
1.10.3.2 Sodium azide
-
 Agrobacterium sp. S5-1
1.10.3.2 Zn2+ partially inhibits laccase activity Agrobacterium sp. S5-1

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.2308
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 30°C, pH 4.5 Agrobacterium sp. S5-1

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.10.3.2 Cu2+ activity is low in the absence of Cu2+, so 1 mM Cu2+ is added into the assay buffer Agrobacterium sp. S5-1

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 Acetone 10% v/v, 10°C, 1 h, 100% of activity remains Agrobacterium sp. S5-1
1.10.3.2 dimethyl sulfoxide 10% v/v, 10°C, 1 h, 72% of activity remains Agrobacterium sp. S5-1
1.10.3.2 Ethanol 10% v/v, 10°C, 1 h, 84% of activity remains Agrobacterium sp. S5-1
1.10.3.2 Methanol 10% v/v, 10°C, 1 h, 95% of activity remains Agrobacterium sp. S5-1

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Agrobacterium sp. S5-1 A0A0F6SIA9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2 recombinant enzyme Agrobacterium sp. S5-1

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.10.3.2 185.4
-
 30°C, pH 4.5, substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Agrobacterium sp. S5-1

Storage Stability

EC Number Storage Stability Organism
1.10.3.2 4°C, accase retained more than 50% activity in the phosphate-buffered saline, stable for 1 week Agrobacterium sp. S5-1
1.10.3.2 4°C, in deionized water, stable for more than 20 days Agrobacterium sp. S5-1

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Agrobacterium sp. S5-1 ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 ATM
-
 Agrobacterium sp. S5-1

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 50
-
-
 Agrobacterium sp. S5-1

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.10.3.2 45 60 45°C: about 20% of maximal activity, 60°C: about 25% of maximal activity Agrobacterium sp. S5-1

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 40
-
 half-life time (t1/s): more than 12 h Agrobacterium sp. S5-1
1.10.3.2 70
-
 half-life time (t1/s): 30 min Agrobacterium sp. S5-1
1.10.3.2 80
-
 10 min, complete inactivation Agrobacterium sp. S5-1

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 32.8
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 30°C, pH 4.5 Agrobacterium sp. S5-1

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 4.5
-
-
 Agrobacterium sp. S5-1

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.10.3.2 2 6 pH 2.0: about 30% of maximal activity, pH 6.0: about 30% of maximal activity Agrobacterium sp. S5-1

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 2
-
 30°C, 40% of enzymatic activities after 1 h treatment Agrobacterium sp. S5-1
1.10.3.2 9
-
 30°C, 5.5% of enzymatic activities after 1 h treatment Agrobacterium sp. S5-1

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.10.3.2 142.1
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) 30°C, pH 4.5 Agrobacterium sp. S5-1