BRENDA - Enzyme Database

Biochemical characterization and substrate specificity of jojoba fatty acyl-CoA reductase and jojoba wax synthase

Miklaszewska, M.; Banas, A.; Plant Sci. 249, 84-92 (2016)

Data extracted from this reference:

Activating Compound
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.84
bovine serum albumin
activates 4fold at 0.5 mg
Simmondsia chinensis
2.3.1.75
bovine serum albumin
highest activity in the presence of 0.6 mg bovine serum albumin
Simmondsia chinensis
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.2.1.84
gene FAR, recombinant expression in Saccharomyces cerevisiae wild-type strain BY4742 and in quadruple mutant strain H1246 lacking genes involved in triacylglycerolsand sterol esters synthesis
Simmondsia chinensis
2.3.1.75
expressed in Saccharomyces cerevisiae quadruple mutant strain H1246
Simmondsia chinensis
General Stability
EC Number
General Stability
Organism
2.3.1.75
wax esters formation is not observed in citrate buffer with pH below 5.5. The enzyme activity at pH 6.0 is higher in citrate buffer than in p-buffer
Simmondsia chinensis
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.84
membrane
a membrane-associated enzyme
Simmondsia chinensis
16020
-
1.2.1.84
microsome
-
Simmondsia chinensis
-
-
2.3.1.75
membrane
-
Simmondsia chinensis
16020
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.84
a long-chain acyl-CoA + 2 NADPH + 2 H+
Simmondsia chinensis
-
a long-chain alcohol + 2 NADP+ + coenzyme A
-
-
?
2.3.1.75
acyl-CoA + a long-chain alcohol
Simmondsia chinensis
-
CoA + a long-chain ester
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.2.1.84
Simmondsia chinensis
Q9XGY7
-
-
2.3.1.75
Simmondsia chinensis
-
-
-
Purification (Commentary)
EC Number
Purification (Commentary)
Organism
1.2.1.84
native enzyme partially by microsomal membrane preparation
Simmondsia chinensis
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.84
embryo
-
Simmondsia chinensis
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.2.1.84
2-methylhexadecanoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
2-methylhexadecanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
2-methyloctadecanoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
2-methyloctadecanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
a long-chain acyl-CoA + 2 NADPH + 2 H+
-
743538
Simmondsia chinensis
a long-chain alcohol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
arachidoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
arachidol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
decanoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
decanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
eicosenoyl-CoA + 2 NADPH + 2 H+
higher activity
743538
Simmondsia chinensis
eicosanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
erucyl-CoA + 2 NADPH + 2 H+
moderate activity
743538
Simmondsia chinensis
erucyl alcohol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
lauroyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
dodecanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
additional information
enzyme substrate specificity, overview. Poor activity with linoleoyl-CoA and linolenoyl-CoA
743538
Simmondsia chinensis
?
-
-
-
?
1.2.1.84
myristoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
myristol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
oleoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
octadecenol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
palmitoyl-CoA + 2 NADPH + 2 H+
lower activity
743538
Simmondsia chinensis
hexadecanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
ricinoleoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
(9Z,12R)-octadec-9-ene-1,12-diol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
stearoyl-CoA + 2 NADPH + 2 H+
best substrate
743538
Simmondsia chinensis
octadecanol + 2 NADP+ + coenzyme A
-
-
-
?
2.3.1.75
acyl-CoA + a long-chain alcohol
-
743538
Simmondsia chinensis
CoA + a long-chain ester
-
-
-
?
2.3.1.75
eicosenoyl-CoA + docosenol
high activity
743538
Simmondsia chinensis
docosenoyl eicosenate + CoA
-
-
-
?
2.3.1.75
eicosenoyl-CoA + dodecanol
high activity
743538
Simmondsia chinensis
dodecanoyl eicosenate + CoA
-
-
-
?
2.3.1.75
eicosenoyl-CoA + hexadecanol
high activity
743538
Simmondsia chinensis
hexadecanyl eicosenate + CoA
-
-
-
?
2.3.1.75
eicosenoyl-CoA + tetradecanol
high activity
743538
Simmondsia chinensis
tetradecyl eicosenate + CoA
-
-
-
?
2.3.1.75
linoleoyl-CoA + dodecanol
high activity
743538
Simmondsia chinensis
dodecanoyl linoleate + CoA
-
-
-
?
2.3.1.75
linoleoyl-CoA + hexadecanol
high activity
743538
Simmondsia chinensis
hexadecanyl linoleate + CoA
-
-
-
?
2.3.1.75
linoleoyl-CoA + tetradecanol
high activity
743538
Simmondsia chinensis
tetradecyl linoleate + CoA
-
-
-
?
2.3.1.75
linoleoyl-CoA + tetradecanol
second highest activity
743538
Simmondsia chinensis
tetradecyl linoleate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + docosenol
-
743538
Simmondsia chinensis
docosenoyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + eicosenol
highest activity
743538
Simmondsia chinensis
eicosenoyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + hexadecanol
high activity
743538
Simmondsia chinensis
hexadecanyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + hexadecenol
high activity
743538
Simmondsia chinensis
hexadecenyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + linolenyl alcohol
high activity
743538
Simmondsia chinensis
linolenoyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + linoleyl alcohol
high activity
743538
Simmondsia chinensis
linoleoyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + octadecanol
high activity
743538
Simmondsia chinensis
octadecyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + octadecenol
high activity
743538
Simmondsia chinensis
octadecenyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + phytol
-
743538
Simmondsia chinensis
phytyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + tetracosenol
-
743538
Simmondsia chinensis
tetracosenyl myristate + CoA
-
-
-
?
2.3.1.75
oleoyl-CoA + tetradecanol
high activity
743538
Simmondsia chinensis
tetradecyl oleate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + eicosenol
high activity
743538
Simmondsia chinensis
eicosenoyl palmitate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + hexadecanol
high activity
743538
Simmondsia chinensis
hexadecanyl palmitate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + hexadecenol
-
743538
Simmondsia chinensis
hexadecenyl palmitate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + octadecanol
high activity
743538
Simmondsia chinensis
octadecyl palmitate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + octadecenol
high activity
743538
Simmondsia chinensis
octadecenyl palmitate + CoA
-
-
-
?
2.3.1.75
stearoyl-CoA + tetradecanol
high activity
743538
Simmondsia chinensis
tetradecyl stearate + CoA
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.2.1.84
?
x * 56200, about, sequence calculation
Simmondsia chinensis
1.2.1.84
More
alcohol-forming fatty acyl-CoA reductases are membrane-associated enzymes, which contain a Rossmann fold NAD(P)H-binding domain and a fatty acyl-CoA reductase (FAR C) domain
Simmondsia chinensis
Synonyms
EC Number
Synonyms
Commentary
Organism
1.2.1.84
alcohol-forming fatty acyl-CoA reductase
-
Simmondsia chinensis
1.2.1.84
FAR
-
Simmondsia chinensis
1.2.1.84
fatty acyl-CoA reductase
-
Simmondsia chinensis
2.3.1.75
wax synthase
-
Simmondsia chinensis
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.84
40
-
-
Simmondsia chinensis
2.3.1.75
35
-
-
Simmondsia chinensis
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.1.84
25
45
over 50% of maximal activity within this range, 50% of maximal activity at 25°C
Simmondsia chinensis
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.84
6.5
-
-
Simmondsia chinensis
2.3.1.75
7
-
the activity of the enzyme gradually increases with the raise of buffer pH reaching the maximum at pH around 7.0
Simmondsia chinensis
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.84
4.5
7.5
inactive at pH 4.0, gradual increase up to maximal activity at pH 6.5, 42% of maximal activity at pH 7.5, reduction of activity above
Simmondsia chinensis
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.84
NADPH
-
Simmondsia chinensis
Activating Compound (protein specific)
EC Number
Activating Compound
Commentary
Organism
Structure
1.2.1.84
bovine serum albumin
activates 4fold at 0.5 mg
Simmondsia chinensis
2.3.1.75
bovine serum albumin
highest activity in the presence of 0.6 mg bovine serum albumin
Simmondsia chinensis
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.84
gene FAR, recombinant expression in Saccharomyces cerevisiae wild-type strain BY4742 and in quadruple mutant strain H1246 lacking genes involved in triacylglycerolsand sterol esters synthesis
Simmondsia chinensis
2.3.1.75
expressed in Saccharomyces cerevisiae quadruple mutant strain H1246
Simmondsia chinensis
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.2.1.84
NADPH
-
Simmondsia chinensis
General Stability (protein specific)
EC Number
General Stability
Organism
2.3.1.75
wax esters formation is not observed in citrate buffer with pH below 5.5. The enzyme activity at pH 6.0 is higher in citrate buffer than in p-buffer
Simmondsia chinensis
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.2.1.84
membrane
a membrane-associated enzyme
Simmondsia chinensis
16020
-
1.2.1.84
microsome
-
Simmondsia chinensis
-
-
2.3.1.75
membrane
-
Simmondsia chinensis
16020
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.2.1.84
a long-chain acyl-CoA + 2 NADPH + 2 H+
Simmondsia chinensis
-
a long-chain alcohol + 2 NADP+ + coenzyme A
-
-
?
2.3.1.75
acyl-CoA + a long-chain alcohol
Simmondsia chinensis
-
CoA + a long-chain ester
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.2.1.84
native enzyme partially by microsomal membrane preparation
Simmondsia chinensis
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.2.1.84
embryo
-
Simmondsia chinensis
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.2.1.84
2-methylhexadecanoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
2-methylhexadecanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
2-methyloctadecanoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
2-methyloctadecanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
a long-chain acyl-CoA + 2 NADPH + 2 H+
-
743538
Simmondsia chinensis
a long-chain alcohol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
arachidoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
arachidol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
decanoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
decanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
eicosenoyl-CoA + 2 NADPH + 2 H+
higher activity
743538
Simmondsia chinensis
eicosanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
erucyl-CoA + 2 NADPH + 2 H+
moderate activity
743538
Simmondsia chinensis
erucyl alcohol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
lauroyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
dodecanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
additional information
enzyme substrate specificity, overview. Poor activity with linoleoyl-CoA and linolenoyl-CoA
743538
Simmondsia chinensis
?
-
-
-
?
1.2.1.84
myristoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
myristol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
oleoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
octadecenol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
palmitoyl-CoA + 2 NADPH + 2 H+
lower activity
743538
Simmondsia chinensis
hexadecanol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
ricinoleoyl-CoA + 2 NADPH + 2 H+
low activity
743538
Simmondsia chinensis
(9Z,12R)-octadec-9-ene-1,12-diol + 2 NADP+ + coenzyme A
-
-
-
?
1.2.1.84
stearoyl-CoA + 2 NADPH + 2 H+
best substrate
743538
Simmondsia chinensis
octadecanol + 2 NADP+ + coenzyme A
-
-
-
?
2.3.1.75
acyl-CoA + a long-chain alcohol
-
743538
Simmondsia chinensis
CoA + a long-chain ester
-
-
-
?
2.3.1.75
eicosenoyl-CoA + docosenol
high activity
743538
Simmondsia chinensis
docosenoyl eicosenate + CoA
-
-
-
?
2.3.1.75
eicosenoyl-CoA + dodecanol
high activity
743538
Simmondsia chinensis
dodecanoyl eicosenate + CoA
-
-
-
?
2.3.1.75
eicosenoyl-CoA + hexadecanol
high activity
743538
Simmondsia chinensis
hexadecanyl eicosenate + CoA
-
-
-
?
2.3.1.75
eicosenoyl-CoA + tetradecanol
high activity
743538
Simmondsia chinensis
tetradecyl eicosenate + CoA
-
-
-
?
2.3.1.75
linoleoyl-CoA + dodecanol
high activity
743538
Simmondsia chinensis
dodecanoyl linoleate + CoA
-
-
-
?
2.3.1.75
linoleoyl-CoA + hexadecanol
high activity
743538
Simmondsia chinensis
hexadecanyl linoleate + CoA
-
-
-
?
2.3.1.75
linoleoyl-CoA + tetradecanol
high activity
743538
Simmondsia chinensis
tetradecyl linoleate + CoA
-
-
-
?
2.3.1.75
linoleoyl-CoA + tetradecanol
second highest activity
743538
Simmondsia chinensis
tetradecyl linoleate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + docosenol
-
743538
Simmondsia chinensis
docosenoyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + eicosenol
highest activity
743538
Simmondsia chinensis
eicosenoyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + hexadecanol
high activity
743538
Simmondsia chinensis
hexadecanyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + hexadecenol
high activity
743538
Simmondsia chinensis
hexadecenyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + linolenyl alcohol
high activity
743538
Simmondsia chinensis
linolenoyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + linoleyl alcohol
high activity
743538
Simmondsia chinensis
linoleoyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + octadecanol
high activity
743538
Simmondsia chinensis
octadecyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + octadecenol
high activity
743538
Simmondsia chinensis
octadecenyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + phytol
-
743538
Simmondsia chinensis
phytyl myristate + CoA
-
-
-
?
2.3.1.75
myristoyl-CoA + tetracosenol
-
743538
Simmondsia chinensis
tetracosenyl myristate + CoA
-
-
-
?
2.3.1.75
oleoyl-CoA + tetradecanol
high activity
743538
Simmondsia chinensis
tetradecyl oleate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + eicosenol
high activity
743538
Simmondsia chinensis
eicosenoyl palmitate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + hexadecanol
high activity
743538
Simmondsia chinensis
hexadecanyl palmitate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + hexadecenol
-
743538
Simmondsia chinensis
hexadecenyl palmitate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + octadecanol
high activity
743538
Simmondsia chinensis
octadecyl palmitate + CoA
-
-
-
?
2.3.1.75
palmitoyl-CoA + octadecenol
high activity
743538
Simmondsia chinensis
octadecenyl palmitate + CoA
-
-
-
?
2.3.1.75
stearoyl-CoA + tetradecanol
high activity
743538
Simmondsia chinensis
tetradecyl stearate + CoA
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.2.1.84
?
x * 56200, about, sequence calculation
Simmondsia chinensis
1.2.1.84
More
alcohol-forming fatty acyl-CoA reductases are membrane-associated enzymes, which contain a Rossmann fold NAD(P)H-binding domain and a fatty acyl-CoA reductase (FAR C) domain
Simmondsia chinensis
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.2.1.84
40
-
-
Simmondsia chinensis
2.3.1.75
35
-
-
Simmondsia chinensis
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.2.1.84
25
45
over 50% of maximal activity within this range, 50% of maximal activity at 25°C
Simmondsia chinensis
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.2.1.84
6.5
-
-
Simmondsia chinensis
2.3.1.75
7
-
the activity of the enzyme gradually increases with the raise of buffer pH reaching the maximum at pH around 7.0
Simmondsia chinensis
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.2.1.84
4.5
7.5
inactive at pH 4.0, gradual increase up to maximal activity at pH 6.5, 42% of maximal activity at pH 7.5, reduction of activity above
Simmondsia chinensis