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Literature summary extracted from

  • Miklaszewska, M.; Banas, A.
    Biochemical characterization and substrate specificity of jojoba fatty acyl-CoA reductase and jojoba wax synthase (2016), Plant Sci., 249, 84-92 .
    View publication on PubMed

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.2.1.84 bovine serum albumin activates 4fold at 0.5 mg Simmondsia chinensis
2.3.1.75 bovine serum albumin highest activity in the presence of 0.6 mg bovine serum albumin Simmondsia chinensis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.84 gene FAR, recombinant expression in Saccharomyces cerevisiae wild-type strain BY4742 and in quadruple mutant strain H1246 lacking genes involved in triacylglycerolsand sterol esters synthesis Simmondsia chinensis
2.3.1.75 expressed in Saccharomyces cerevisiae quadruple mutant strain H1246 Simmondsia chinensis

General Stability

EC Number General Stability Organism
2.3.1.75 wax esters formation is not observed in citrate buffer with pH below 5.5. The enzyme activity at pH 6.0 is higher in citrate buffer than in p-buffer Simmondsia chinensis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.2.1.84 membrane a membrane-associated enzyme Simmondsia chinensis 16020
-
1.2.1.84 microsome
-
 Simmondsia chinensis
-
-
2.3.1.75 membrane
-
 Simmondsia chinensis 16020
-

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.84 a long-chain acyl-CoA + 2 NADPH + 2 H+ Simmondsia chinensis
-
 a long-chain alcohol + 2 NADP+ + coenzyme A
-
 ?
2.3.1.75 acyl-CoA + a long-chain alcohol Simmondsia chinensis
-
 CoA + a long-chain ester
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.84 Simmondsia chinensis Q9XGY7
-
-
2.3.1.75 Simmondsia chinensis
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.84 native enzyme partially by microsomal membrane preparation Simmondsia chinensis

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.2.1.84 embryo
-
 Simmondsia chinensis
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.84 2-methylhexadecanoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis 2-methylhexadecanol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 2-methyloctadecanoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis 2-methyloctadecanol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 a long-chain acyl-CoA + 2 NADPH + 2 H+
-
 Simmondsia chinensis a long-chain alcohol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 arachidoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis arachidol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 decanoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis decanol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 eicosenoyl-CoA + 2 NADPH + 2 H+ higher activity Simmondsia chinensis eicosanol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 erucyl-CoA + 2 NADPH + 2 H+ moderate activity Simmondsia chinensis erucyl alcohol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 lauroyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis dodecanol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 additional information enzyme substrate specificity, overview. Poor activity with linoleoyl-CoA and linolenoyl-CoA Simmondsia chinensis ?
-
 ?
1.2.1.84 myristoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis myristol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 oleoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis octadecenol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 palmitoyl-CoA + 2 NADPH + 2 H+ lower activity Simmondsia chinensis hexadecanol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 ricinoleoyl-CoA + 2 NADPH + 2 H+ low activity Simmondsia chinensis (9Z,12R)-octadec-9-ene-1,12-diol + 2 NADP+ + coenzyme A
-
 ?
1.2.1.84 stearoyl-CoA + 2 NADPH + 2 H+ best substrate Simmondsia chinensis octadecanol + 2 NADP+ + coenzyme A
-
 ?
2.3.1.75 acyl-CoA + a long-chain alcohol
-
 Simmondsia chinensis CoA + a long-chain ester
-
 ?
2.3.1.75 eicosenoyl-CoA + docosenol high activity Simmondsia chinensis docosenyl eicosenoate + CoA
-
 ?
2.3.1.75 eicosenoyl-CoA + dodecanol high activity Simmondsia chinensis dodecyl eicosenoate + CoA
-
 ?
2.3.1.75 eicosenoyl-CoA + hexadecanol high activity Simmondsia chinensis hexadecanyl eicosenate + CoA
-
 ?
2.3.1.75 eicosenoyl-CoA + tetradecanol high activity Simmondsia chinensis tetradecyl eicosenoate + CoA
-
 ?
2.3.1.75 linoleoyl-CoA + dodecanol high activity Simmondsia chinensis dodecyl linoleate + CoA
-
 ?
2.3.1.75 linoleoyl-CoA + hexadecanol high activity Simmondsia chinensis hexadecanyl linoleate + CoA
-
 ?
2.3.1.75 linoleoyl-CoA + tetradecanol high activity Simmondsia chinensis tetradecyl linoleate + CoA
-
 ?
2.3.1.75 linoleoyl-CoA + tetradecanol second highest activity Simmondsia chinensis tetradecyl linoleate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + docosenol
-
 Simmondsia chinensis docosenyl myristate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + eicosenol highest activity Simmondsia chinensis eicosenyl myristate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + hexadecanol high activity Simmondsia chinensis hexadecanyl myristate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + hexadecenol high activity Simmondsia chinensis hexadecenyl myristate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + linolenyl alcohol high activity Simmondsia chinensis linolenoyl myristate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + linoleyl alcohol high activity Simmondsia chinensis linoleoyl myristate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + octadecanol high activity Simmondsia chinensis octadecyl myristate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + octadecenol high activity Simmondsia chinensis octadecenyl myristate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + phytol
-
 Simmondsia chinensis phytyl myristate + CoA
-
 ?
2.3.1.75 myristoyl-CoA + tetracosenol
-
 Simmondsia chinensis tetracosenyl myristate + CoA
-
 ?
2.3.1.75 oleoyl-CoA + tetradecanol high activity Simmondsia chinensis tetradecyl oleate + CoA
-
 ?
2.3.1.75 palmitoyl-CoA + eicosenol high activity Simmondsia chinensis eicosenyl palmitate + CoA
-
 ?
2.3.1.75 palmitoyl-CoA + hexadecanol high activity Simmondsia chinensis hexadecanyl palmitate + CoA
-
 ?
2.3.1.75 palmitoyl-CoA + hexadecenol
-
 Simmondsia chinensis hexadecenyl palmitate + CoA
-
 ?
2.3.1.75 palmitoyl-CoA + octadecanol high activity Simmondsia chinensis octadecyl palmitate + CoA
-
 ?
2.3.1.75 palmitoyl-CoA + octadecenol high activity Simmondsia chinensis octadecenyl palmitate + CoA
-
 ?
2.3.1.75 stearoyl-CoA + tetradecanol high activity Simmondsia chinensis tetradecyl stearate + CoA
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.84 ? x * 56200, about, sequence calculation Simmondsia chinensis
1.2.1.84 More alcohol-forming fatty acyl-CoA reductases are membrane-associated enzymes, which contain a Rossmann fold NAD(P)H-binding domain and a fatty acyl-CoA reductase (FAR C) domain Simmondsia chinensis

Synonyms

EC Number Synonyms Comment Organism
1.2.1.84 alcohol-forming fatty acyl-CoA reductase
-
 Simmondsia chinensis
1.2.1.84 FAR
-
 Simmondsia chinensis
1.2.1.84 fatty acyl-CoA reductase
-
 Simmondsia chinensis
2.3.1.75 wax synthase
-
 Simmondsia chinensis

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.84 40
-
-
 Simmondsia chinensis
2.3.1.75 35
-
-
 Simmondsia chinensis

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.2.1.84 25 45 over 50% of maximal activity within this range, 50% of maximal activity at 25°C Simmondsia chinensis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.84 6.5
-
-
 Simmondsia chinensis
2.3.1.75 7
-
 the activity of the enzyme gradually increases with the raise of buffer pH reaching the maximum at pH around 7.0 Simmondsia chinensis

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.84 4.5 7.5 inactive at pH 4.0, gradual increase up to maximal activity at pH 6.5, 42% of maximal activity at pH 7.5, reduction of activity above Simmondsia chinensis

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.84 NADPH
-
 Simmondsia chinensis