EC Number | Application | Comment | Organism |
---|---|---|---|
4.2.1.173 | synthesis | CPS4 and kaurene synthase-like2 act together to produce ent-13-epi-manoyl oxide | Salvia miltiorrhiza |
EC Number | Cloned (Comment) | Organism |
---|---|---|
4.2.1.173 | - |
Salvia miltiorrhiza |
4.2.3.186 | - |
Salvia miltiorrhiza f. alba |
5.5.1.12 | gene SmCPS1, phylogenetic analysis, a 289-bp gene-specific sequence including the 3' UTR of SmCPS1 is amplified by PCR using cDNA as a template and then cloned into the pK7GWIWG (II) binary, then introduced into Agrobacterium tumefaciens strain EHA105 by electroporation for ransformation of leaf explants from Salvia miltiorrhiza bh2-7 plants, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3) | Salvia miltiorrhiza f. alba |
5.5.1.12 | gene SmCPS2, phylogenetic analysis, recombinant expression of His-tagged enzyme in Escherichia coli strains Tuner (DE3) or Origami B (DE3) | Salvia miltiorrhiza f. alba |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
5.5.1.12 | additional information | knockdown of SmCPS1 by RNAi | Salvia miltiorrhiza f. alba |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
5.5.1.12 | geranylgeranyl diphosphate | Salvia miltiorrhiza f. alba | - |
(+)-copalyl diphosphate | - |
r | |
5.5.1.12 | geranylgeranyl diphosphate | Salvia miltiorrhiza f. alba bh2-7 | - |
(+)-copalyl diphosphate | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.2.1.173 | Salvia miltiorrhiza | A0A0U2D9C5 | f. alba | - |
4.2.3.186 | Salvia miltiorrhiza f. alba | A0A0A7ANA5 | - |
- |
5.5.1.12 | Salvia miltiorrhiza f. alba | A0A0A7AN31 | - |
- |
5.5.1.12 | Salvia miltiorrhiza f. alba | A0A0A7ANT1 | - |
- |
5.5.1.12 | Salvia miltiorrhiza f. alba bh2-7 | A0A0A7AN31 | - |
- |
5.5.1.12 | Salvia miltiorrhiza f. alba bh2-7 | A0A0A7ANT1 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
5.5.1.12 | recombinant His-tagged enzyme by nickel affinity chromatography from Escherichia coli strains Tuner (DE3) or Origami B (DE3) | Salvia miltiorrhiza f. alba |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
4.2.1.173 | sepal | most highly expressed | Salvia miltiorrhiza | - |
4.2.3.186 | additional information | expressed throughout all tissues of adult plants | Salvia miltiorrhiza f. alba | - |
4.2.3.186 | root | most highly expressed in root xylem | Salvia miltiorrhiza f. alba | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.2.1.173 | geranylgeranyl diphosphate + H2O | - |
Salvia miltiorrhiza | ent-8alpha-hydroxylabd-13-en-15-yl diphosphate | - |
? | |
4.2.3.186 | ent-8alpha-hydroxylabd-13-en-15-yl diphosphate | - |
Salvia miltiorrhiza f. alba | ent-13-epi-manoyl oxide + diphosphate | - |
? | |
4.2.3.186 | additional information | in presence of geranylgeranyl diphosphate, combining copalyl diphosphate synthase CPS4 and KSL2 leads to production of ent-13-epi-manoyl oxide. No product is observed when CPS1, CPS2 or CPS3 is combined with KSL2 | Salvia miltiorrhiza f. alba | ? | - |
? | |
5.5.1.12 | geranylgeranyl diphosphate | - |
Salvia miltiorrhiza f. alba | (+)-copalyl diphosphate | - |
r | |
5.5.1.12 | geranylgeranyl diphosphate | - |
Salvia miltiorrhiza f. alba bh2-7 | (+)-copalyl diphosphate | - |
r |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.2.1.173 | copal-8-ol diphosphate synthase | - |
Salvia miltiorrhiza |
4.2.1.173 | CPS4 | - |
Salvia miltiorrhiza |
4.2.3.186 | KSL2 | - |
Salvia miltiorrhiza f. alba |
5.5.1.12 | CPS1 | - |
Salvia miltiorrhiza f. alba |
5.5.1.12 | CPS2 | - |
Salvia miltiorrhiza f. alba |
5.5.1.12 | SmCPS1 | - |
Salvia miltiorrhiza f. alba |
5.5.1.12 | SmCPS2 | - |
Salvia miltiorrhiza f. alba |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
5.5.1.12 | 25 | - |
assay at | Salvia miltiorrhiza f. alba |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
5.5.1.12 | 8 | - |
assay at | Salvia miltiorrhiza f. alba |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.2.3.186 | physiological function | KSL2 may have dual function both in gibberlinic acid and in diterpenoid biosynthesis | Salvia miltiorrhiza f. alba |
5.5.1.12 | evolution | the enzyme belongs to the diterpene synthase family | Salvia miltiorrhiza f. alba |
5.5.1.12 | additional information | metabolomic profiling, and homology modeling and molecular docking | Salvia miltiorrhiza f. alba |