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Literature summary extracted from

  • Cai, X.; Zhang, C.; Ye, J.; Hu, T.; Ye, Z.; Li, H.; Zhang, Y.
    Ectopic expression of FaGalUR leads to ascorbate accumulation with enhanced oxidative stress, cold, and salt tolerance in tomato (2015), Plant Growth Regul., 76, 187-197 .
No PubMed abstract available

Application

EC Number Application Comment Organism
1.1.1.365 nutrition accumulation of high levels of ascorbate using GalUR gene overepression in tomato fruits. Tomato fruits are considered a major dietary source of vitamin C in many countries, because it is consumed regularly and in large quantities. Tomato also serves as a fruit model for other crops species with fleshy berry. Accordingly, it is very important to monitor and to increase the vitamin C content in tomato fruit, for meeting the consumer demand and health requirements for high nutrition, e.g. by overexpressing D-galacturonate reductase Fragaria x ananassa

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.365 gene GalUR, recombinant overexpression in Solanum lycopersicum cv. Ailsa Craig leaves and fruits via Agrobacterium tumefaciens strain C58C1 transfection resulting in 2fold and 1.6fold increase in ascorbate level in tomato fruit and leaf, respectively, which correlates positively with FaGalUR transcriptional abundance and enzyme GalUR activity compared to wild-type plants, real-time PCR expression analysis Fragaria x ananassa

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.365 additional information gene FaGalUR-overexpressing Solanum lycopersicum plants show enhanced ascorbic acid levels, tolerance to abiotic stresses induced by oxidization (methyl viologen), salt (NaCl), and cold compared to the wild-type plants, overview. Ascorbate accumulation in tomato can be enhanced by regulating Fragaria x ananassa GalUR gene Fragaria x ananassa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.365 D-galacturonate + NADPH + H+ Fragaria x ananassa
-
 L-galactonate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.365 Fragaria x ananassa O49133 cv. Fenglaixiang, Fragaria chiloensis x Fragaria virginiana
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.1.1.365 fruit
-
 Fragaria x ananassa
-
1.1.1.365 leaf
-
 Fragaria x ananassa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.365 D-galacturonate + NADPH + H+
-
 Fragaria x ananassa L-galactonate + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.1.1.365 FaGalUR
-
 Fragaria x ananassa
1.1.1.365 GalUR
-
 Fragaria x ananassa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.365 25
-
 assay at Fragaria x ananassa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.365 7.2
-
 assay at Fragaria x ananassa

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.365 NADP+
-
 Fragaria x ananassa
1.1.1.365 NADPH
-
 Fragaria x ananassa

General Information

EC Number General Information Comment Organism
1.1.1.365 physiological function the D-galacturonic acid reductase catalyzes the conversion of D-galacturonic acid to L-galactonic acid in plant. The D-galacturonic acid, known as an abundant component of the cell wall, is a degradation product of pectin in senescencing plant cells. The expression levels of FaGalUR are correlated with increased ascorbate content in ripe strawberry fruit. Fragaria x ananassa gene FaGalUR expression improves salt and cold tolerance in Solanum lycopersicum fruits, and improves tolerance to oxidative stress in tomato Fragaria x ananassa