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Literature summary extracted from

  • Liu, T.; Kim, D.W.; Niitsu, M.; Berberich, T.; Kusano, T.
    Oryza sativa polyamine oxidase 1 back-converts tetraamines, spermine and thermospermine, to spermidine (2014), Plant Cell Rep., 33, 143-151 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.17 expression in Escherichia coli Oryza sativa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.3.17 0.0037
-
 thermospermine pH 6.0, 30°C Oryza sativa
1.5.3.17 0.0049
-
 spermine pH 6.0, 30°C Oryza sativa
1.5.3.17 0.0061
-
 spermine pH 8.5, 30°C Oryza sativa
1.5.3.17 0.011
-
 norspermine pH 6.0, 30°C Oryza sativa
1.5.3.17 0.0145
-
 norspermine pH 8.5, 30°C Oryza sativa
1.5.3.17 0.0359
-
 N1-acetylspermine pH 8.5, 30°C Oryza sativa

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.5.3.17 cytoplasm
-
 Oryza sativa 5737
-

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.17 Oryza sativa
-
-
-
1.5.3.17 Oryza sativa cv. Nipponbare
-
-
-

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.5.3.17 root of seedlings Oryza sativa
-
1.5.3.17 seedling root of seedlings Oryza sativa
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.17 N1-acetylspermine + O2 + H2O
-
 Oryza sativa spermidine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.17 N1-acetylspermine + O2 + H2O
-
 Oryza sativa cv. Nipponbare spermidine + 3-acetamidopropanal + H2O2
-
 ?
1.5.3.17 norspermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa ?
-
 ?
1.5.3.17 norspermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa cv. Nipponbare ?
-
 ?
1.5.3.17 spermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa spermidine + 3-aminopropanal + H2O2
-
 ?
1.5.3.17 spermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa cv. Nipponbare spermidine + 3-aminopropanal + H2O2
-
 ?
1.5.3.17 thermospermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa ?
-
 ?
1.5.3.17 thermospermine + O2 + H2O the recombinant OsPAO1 enzyme prefers therospermine as a substrate at pH 6.0 and spermine at pH 8.5 Oryza sativa cv. Nipponbare ?
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.5.3.17 OsPAO1
-
 Oryza sativa
1.5.3.17 polyamine oxidase 1
-
 Oryza sativa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.17 30
-
 substrate: therospermine Oryza sativa
1.5.3.17 35
-
 substrate: spermine Oryza sativa

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.3.17 0.018
-
 norspermine pH 6.0, 30°C Oryza sativa
1.5.3.17 0.022
-
 N1-acetylspermine pH 8.5, 30°C Oryza sativa
1.5.3.17 0.025
-
 spermine pH 6.0, 30°C Oryza sativa
1.5.3.17 0.036
-
 norspermine pH 8.5, 30°C Oryza sativa
1.5.3.17 0.043
-
 spermine pH 8.5, 30°C Oryza sativa
1.5.3.17 0.051
-
 thermospermine pH 6.0, 30°C Oryza sativa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.3.17 6
-
 substrate: thermospermine Oryza sativa
1.5.3.17 8.5
-
 spermine Oryza sativa

Expression

EC Number Organism Comment Expression
1.5.3.17 Oryza sativa expression of the enzyme OsPAO1 appears to be quite low under physiological conditions, but is markedly induced in rice roots by spermine or thermospermine treatment up

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.3.17 0.61
-
 N1-acetylspermine pH 8.5, 30°C Oryza sativa
1.5.3.17 1.64
-
 norspermine pH 6.0, 30°C Oryza sativa
1.5.3.17 2.48
-
 norspermine pH 8.5, 30°C Oryza sativa
1.5.3.17 5.1
-
 spermine pH 6.0, 30°C Oryza sativa
1.5.3.17 6.4
-
 spermine pH 8.5, 30°C Oryza sativa
1.5.3.17 13.8
-
 thermospermine pH 6.0, 30°C Oryza sativa