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Literature summary extracted from
- Enhanced ascorbate regeneration via dehydroascorbate reductase confers tolerance to photo-oxidative stress in Chlamydomonas reinhardtii (2016), Plant Cell Physiol., 57, 2104-2121 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | expressed in Escherichia coli C41(DE3) cells | Chlamydomonas reinhardtii |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 1.8.5.1 | chloroplast | - |
Chlamydomonas reinhardtii | 9507 | - |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.1 | glutathione + dehydroascorbate | Chlamydomonas reinhardtii | - |
glutathione disulfide + ascorbate | - |
? |  |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.1 | Chlamydomonas reinhardtii | A8I0K9 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.5.1 | Ni-NTA column chromatography | Chlamydomonas reinhardtii |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.8.5.1 | 965 | - |
recombinant enzyme, at pH 8.0, temperature not specified in the publication | Chlamydomonas reinhardtii |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.1 | glutathione + dehydroascorbate | - |
Chlamydomonas reinhardtii | glutathione disulfide + ascorbate | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | monomer | 1 * 25000, SDS-PAGE | Chlamydomonas reinhardtii |
| 1.8.5.1 | monomer | 1 * 24897, calculated from amino acid sequence | Chlamydomonas reinhardtii |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | dehydroascorbate reductase | - |
Chlamydomonas reinhardtii |
| 1.8.5.1 | DHAR1 | - |
Chlamydomonas reinhardtii |
General Information
| EC Number | General Information | Comment | Organism |
|---|---|---|---|
| 1.8.5.1 | physiological function | enzyme overexpression AsA pool size, AsA:DHA ratio and the tolerance to moderate light-, high light-, methyl viologen- or H2O2-induced oxidative stress | Chlamydomonas reinhardtii |
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Release 2023.1 (January 2023)
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