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Literature summary extracted from

  • Wuddineh, W.A.; Mazarei, M.; Zhang, J.; Poovaiah, C.R.; Mann, D.G.; Ziebell, A.; Sykes, R.W.; Davis, M.F.; Udvardi, M.K.; Stewart, C.N.
    Identification and overexpression of gibberellin 2-oxidase (GA2ox) in switchgrass (Panicum virgatum L.) for improved plant architecture and reduced biomass ecalcitrance (2015), Plant Biotechnol. J., 13, 636-647 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.14.11.13 biofuel production overexpression of GA2ox genes in switchgrass is a feasible strategy to improve plant architecture and reduce biomass recalcitrance for biofuel Panicum virgatum

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.11.13 PvGA2ox5b and PvGA2ox9a are cloned from cDNA and constitutively overexpressed in switchgrass under the control of maize ubiquitin promoter Panicum virgatum

Organism

EC Number Organism UniProt Comment Textmining
1.14.11.13 Panicum virgatum
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Synonyms

EC Number Synonyms Comment Organism
1.14.11.13 GA2ox
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 Panicum virgatum
1.14.11.13 gibberellin 2-oxidase
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 Panicum virgatum
1.14.11.13 PvGA2ox5b
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 Panicum virgatum
1.14.11.13 PvGA2ox9a
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 Panicum virgatum

Expression

EC Number Organism Comment Expression
1.14.11.13 Panicum virgatum overexpression of two gibberellin 2beta-dioxygenase genes, that is PvGA2ox5 and PvGA2ox9, results in characteristic gibberellin-deficient phenotypes with dark-green leaves and modified plant architecture. The changes in plant morphology appear to be associated with GA2ox transcript abundance. Exogenous application of GA rescues the GA-deficient phenotypes in transgenic lines. Transgenic semi-dwarf lines display increased tillering and reduced lignin content, and the syringyl/guaiacyl lignin monomer ratio accompanied by the reduced expression of lignin biosynthetic genes compared to nontransgenic plants. A moderate increase in the level of glucose release in these transgenic lines might be attributed to reduced biomass recalcitrance as a result of reduced lignin content and lignin composition. Overexpression of GA2ox genes in switchgrass is a feasible strategy to improve plant architecture and reduce biomass recalcitrance for biofuel up