BRENDA - Enzyme Database show

The 9-lipoxygenase Osr9-LOX1 interacts with the 13-lipoxygenase-mediated pathway to regulate resistance to chewing and piercing-sucking herbivores in rice

Zhou, G.; Ren, N.; Qi, J.; Lu, J.; Xiang, C.; Ju, H.; Cheng, J.; Lou, Y.; Physiol. Plant. 152, 59-69 (2014)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.13.11.58
gene lox1, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, recombinant expression of EGFP-tagged enzyme in Nicotiana tabacum under control of the the CaMV35S promoter
Oryza sativa Japonica Group
Engineering
EC Number
Amino acid exchange
Commentary
Organism
1.13.11.58
additional information
antisense expression of Osr9-lox1 (asr9lox1), by Agrobacterium tumefaciens-mediated transformation, decrease the amount of wound-induced (Z)-3-hexenal but increase levels of striped stem borer (SSB)-induced linolenic acid, lasmonic acid, salicylic acid and trypsin protease inhibitors
Oryza sativa Japonica Group
Localization
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.58
chloroplast
subcellular localization analysis
Oryza sativa Japonica Group
9507
-
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.12
alpha-linolenate + O2
Gracilaria dura
-
(9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
-
-
?
1.13.11.12
linoleic acid + O2
Gracilaria dura
-
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.13.11.12
Gracilaria dura
-
-
-
1.13.11.58
Oryza sativa Japonica Group
Q76I22
cv. Xiushui 11
-
Source Tissue
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.58
leaf
low expression level
Oryza sativa Japonica Group
-
1.13.11.58
additional information
low constitutive expression of Osr9-LOX1 in rice leaves, stems, and spikelets, but high constitutive expression in roots, expression analysis
Oryza sativa Japonica Group
-
1.13.11.58
root
high expression level
Oryza sativa Japonica Group
-
1.13.11.58
seedling
-
Oryza sativa Japonica Group
-
1.13.11.58
spikelet
low expression level
Oryza sativa Japonica Group
-
1.13.11.58
stem
low expression level
Oryza sativa Japonica Group
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenate + O2
-
743426
Gracilaria dura
(9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
-
-
-
?
1.13.11.12
linoleic acid + O2
-
743426
Gracilaria dura
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
-
?
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.13.11.58
gene lox1, DNA and amino acid sequence determination and analysis, quantitative real-time PCR expression analysis, recombinant expression of EGFP-tagged enzyme in Nicotiana tabacum under control of the the CaMV35S promoter
Oryza sativa Japonica Group
Engineering (protein specific)
EC Number
Amino acid exchange
Commentary
Organism
1.13.11.58
additional information
antisense expression of Osr9-lox1 (asr9lox1), by Agrobacterium tumefaciens-mediated transformation, decrease the amount of wound-induced (Z)-3-hexenal but increase levels of striped stem borer (SSB)-induced linolenic acid, lasmonic acid, salicylic acid and trypsin protease inhibitors
Oryza sativa Japonica Group
Localization (protein specific)
EC Number
Localization
Commentary
Organism
GeneOntology No.
Textmining
1.13.11.58
chloroplast
subcellular localization analysis
Oryza sativa Japonica Group
9507
-
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.13.11.12
alpha-linolenate + O2
Gracilaria dura
-
(9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
-
-
?
1.13.11.12
linoleic acid + O2
Gracilaria dura
-
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
?
Source Tissue (protein specific)
EC Number
Source Tissue
Commentary
Organism
Textmining
1.13.11.58
leaf
low expression level
Oryza sativa Japonica Group
-
1.13.11.58
additional information
low constitutive expression of Osr9-LOX1 in rice leaves, stems, and spikelets, but high constitutive expression in roots, expression analysis
Oryza sativa Japonica Group
-
1.13.11.58
root
high expression level
Oryza sativa Japonica Group
-
1.13.11.58
seedling
-
Oryza sativa Japonica Group
-
1.13.11.58
spikelet
low expression level
Oryza sativa Japonica Group
-
1.13.11.58
stem
low expression level
Oryza sativa Japonica Group
-
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.13.11.12
alpha-linolenate + O2
-
743426
Gracilaria dura
(9Z,11E,13S,15Z)-13-hydroperoxyoctadeca-9,11,15-trienoate
-
-
-
?
1.13.11.12
linoleic acid + O2
-
743426
Gracilaria dura
(9Z,11E,13S)-13-hydroperoxy-9,11-octadecadienoate
-
-
-
?
Expression
EC Number
Organism
Commentary
Expression
1.13.11.12
Gracilaria dura
methyljasmonate-induced oxidative burst, causing considerable oxidative stress, leads to the induction of a fatty acid oxidation cascade, resulting in the synthesis of hydroxy-oxylipins and the upregulation of the 13-lipoxygenase pathway
up
1.13.11.58
Oryza sativa Japonica Group
herbivore infestation, mechanical wounding and jasmonic acid treatment either repress or do not enhance the level of Osr9-LOX1 transcripts at early stages but do at later stages; herbivore infestation, mechanical wounding and jasmonic acid treatment either repress or do not enhance the level of Osr9-LOX1 transcripts at early stages but do at later stages, whereas salicylic acid (SA) treatment quickly increases the transcript level of Osr9-LOX1
up
General Information
EC Number
General Information
Commentary
Organism
1.13.11.12
metabolism
13-hydroxy-9,11,5-octadecatrienoic acid (13-HOTrE) is produced by the reduction of a 13-LOX product, 13-hydroperoxy-9,11,15-octadecatrienoic acid (by peroxidases), one of the key substrates of jasmonate biosynthesis
Gracilaria dura
1.13.11.58
malfunction
antisense expression of Osr9-lox1 (asr9lox1) decrease the amount of wound-induced (Z)-3-hexenal but increase levels of striped stem borer (SSB)-induced linolenic acid, jasmonic acid, salicylic acid and trypsin protease inhibitors. These changes are associated with increased resistance in rice to the larvae of the SSB Chilo suppressalis. Silencing Osr9-LOX1 results in increased jasmonic acid, salicylic acid and green leaf volatiles ((Z)-3-hexenal) levels
Oryza sativa Japonica Group
1.13.11.58
physiological function
enzyme Osr9-LOX1 plays an important role in regulating an herbivore-induced jasmonic acid burst and cross-talk between jasmonic acid and salicylic acid, and in controlling resistance in rice to chewing and phloem-feeding herbivores
Oryza sativa Japonica Group
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.13.11.12
metabolism
13-hydroxy-9,11,5-octadecatrienoic acid (13-HOTrE) is produced by the reduction of a 13-LOX product, 13-hydroperoxy-9,11,15-octadecatrienoic acid (by peroxidases), one of the key substrates of jasmonate biosynthesis
Gracilaria dura
1.13.11.58
malfunction
antisense expression of Osr9-lox1 (asr9lox1) decrease the amount of wound-induced (Z)-3-hexenal but increase levels of striped stem borer (SSB)-induced linolenic acid, jasmonic acid, salicylic acid and trypsin protease inhibitors. These changes are associated with increased resistance in rice to the larvae of the SSB Chilo suppressalis. Silencing Osr9-LOX1 results in increased jasmonic acid, salicylic acid and green leaf volatiles ((Z)-3-hexenal) levels
Oryza sativa Japonica Group
1.13.11.58
physiological function
enzyme Osr9-LOX1 plays an important role in regulating an herbivore-induced jasmonic acid burst and cross-talk between jasmonic acid and salicylic acid, and in controlling resistance in rice to chewing and phloem-feeding herbivores
Oryza sativa Japonica Group
Expression (protein specific)
EC Number
Organism
Commentary
Expression
1.13.11.12
Gracilaria dura
methyljasmonate-induced oxidative burst, causing considerable oxidative stress, leads to the induction of a fatty acid oxidation cascade, resulting in the synthesis of hydroxy-oxylipins and the upregulation of the 13-lipoxygenase pathway
up
1.13.11.58
Oryza sativa Japonica Group
herbivore infestation, mechanical wounding and jasmonic acid treatment either repress or do not enhance the level of Osr9-LOX1 transcripts at early stages but do at later stages; herbivore infestation, mechanical wounding and jasmonic acid treatment either repress or do not enhance the level of Osr9-LOX1 transcripts at early stages but do at later stages, whereas salicylic acid (SA) treatment quickly increases the transcript level of Osr9-LOX1
up