EC Number | Cloned (Comment) | Organism |
---|---|---|
4.1.99.16 | gene sce1440, recombinant expression in Escherichia coli | Sorangium cellulosum |
4.2.3.175 | expression in Escherichia coli | Sorangium cellulosum |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O | Sorangium cellulosum | - |
(-)-geosmin + acetone | - |
? | |
4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O | Sorangium cellulosum Soce56 | - |
(-)-geosmin + acetone | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
4.1.99.16 | Sorangium cellulosum | A9F845 | - |
- |
4.1.99.16 | Sorangium cellulosum Soce56 | A9F845 | - |
- |
4.2.3.175 | Sorangium cellulosum | A9GK58 | - |
- |
4.2.3.175 | Sorangium cellulosum So ce56 | A9GK58 | - |
- |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O | - |
Sorangium cellulosum | (-)-geosmin + acetone | - |
? | |
4.1.99.16 | (1E,4S,5E,7R)-germacra-1(10),5-dien-11-ol + H2O | - |
Sorangium cellulosum Soce56 | (-)-geosmin + acetone | - |
? | |
4.1.99.16 | additional information | the geosmin synthase sce1440 from Sorangium cellulosum strain Soce56 is very substrate-specific | Sorangium cellulosum | ? | - |
? | |
4.1.99.16 | additional information | the geosmin synthase sce1440 from Sorangium cellulosum strain Soce56 is very substrate-specific | Sorangium cellulosum Soce56 | ? | - |
? | |
4.2.3.175 | (2E,6E)-farnesyl diphosphate + H2O | - |
Sorangium cellulosum | 10-epi-cubebol + diphosphate | - |
? | |
4.2.3.175 | (2E,6E)-farnesyl diphosphate + H2O | - |
Sorangium cellulosum So ce56 | 10-epi-cubebol + diphosphate | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
4.1.99.16 | sce1440 | - |
Sorangium cellulosum |
4.2.3.175 | sce6369 | - |
Sorangium cellulosum |
EC Number | General Information | Comment | Organism |
---|---|---|---|
4.1.99.16 | evolution | identification of Sesquiterpene synthase orthologues of sce1440 in the genomes of myxobacterial strains, overview | Sorangium cellulosum |
4.1.99.16 | metabolism | the enzyme is one of only three type I terpene synthases (TSs, sce1440, sce6369, sce8552) and one type II TS (sce4636), responsible for the production of at least 17 sesquiterpenes in Sorangium cellulosum | Sorangium cellulosum |
4.2.3.175 | physiological function | feeding mevalonolactone to a sce6369 expressing Escherichia coli strain results in a mixture of 21 sesquiterpene products. Structurally identified sesquiterpenes have all cadalane- and cubebane-type scaffolds with the exception of germacrene D. Main product is 10-epi-cubebol | Sorangium cellulosum |