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Literature summary extracted from

  • Gennaris, A.; Ezraty, B.; Henry, C.; Agrebi, R.; Vergnes, A.; Oheix, E.; Bos, J.; Leverrier, P.; Espinosa, L.; Szewczyk, J.; Vertommen, D.; Iranzo, O.; Collet, J.F.; Barras, F.
    Repairing oxidized proteins in the bacterial envelope using respiratory chain electrons (2015), Nature, 528, 409-412 .
    View publication on PubMedView publication on EuropePMC

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.8.5.B1 3.8
-
 N-acetyl-L-methionine (S)-sulfoxide pH 7.0, 30°C Escherichia coli
1.8.5.B1 8
-
 L-methionine (S)-S-oxide pH 7.0, 30°C Escherichia coli
1.8.5.B1 25.7
-
 L-methionine (S)-S-oxide pH 7.0, 30°C Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.B1 periplasm
-
 Escherichia coli
-
-

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.B1 Escherichia coli P76342 and P76343 P76342 i.e. catalytic subunit MsrP, P76343 i.e. heme-binding subunit MsrQ
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.B1 L-methionine (R)-S-oxide + reduced benzyl viologen
-
 Escherichia coli L-methionine + oxidized benzyl viologen + H2O
-
 ?
1.8.5.B1 L-methionine (S)-S-oxide + reduced benzyl viologen
-
 Escherichia coli L-methionine + oxidized benzyl viologen + H2O
-
 ?
1.8.5.B1 L-methionine S-oxide in chaperone SurA + reduced benzyl viologen
-
 Escherichia coli L-methionine in chaperone SurA + oxidized benzyl viologen + H2O
-
 ?
1.8.5.B1 L-methionine S-oxide in lipoprotein Pal + reduced benzyl viologen
-
 Escherichia coli L-methionine in lipoprotein Pal + oxidized benzyl viologen + H2O
-
 ?
1.8.5.B1 additional information MsrPQ is able to reduce both R- and S-diastereoisomers of methionine sulfoxide Escherichia coli ?
-
 ?
1.8.5.B1 N-acetyl-L-methionine (S)-sulfoxide + reduced benzyl viologen
-
 Escherichia coli N-acetyl-L-methionine + oxidized benzyl viologen + H2O
-
 ?
1.8.5.B1 oxidized calmodulin + reduced benzyl viologen
-
 Escherichia coli reduced calmodulin + oxidized benzyl viologen + H2O
-
 ?

Expression

EC Number Organism Comment Expression
1.8.5.B1 Escherichia coli MsrPQ synthesis is induced by hypochlorous acid up

General Information

EC Number General Information Comment Organism
1.8.5.B1 physiological function system MsrPQ repairs proteins containing methionine sulfoxide in the bacterial cell envelope rescuing a series of structurally unrelated periplasmic proteins from methionine oxidation. MsrPQ uses electrons from the respiratory chain. MsrPQ is essential for the maintenance of envelope integrity under bleach stress Escherichia coli