Literature summary extracted from

  • Albesa-Jove, D.; Svetlikova, Z.; Tersa, M.; Sancho-Vaello, E.; Carreras-Gonzalez, A.; Bonnet, P.; Arrasate, P.; Eguskiza, A.; Angala, S.K.; Cifuente, J.O.; Kordulakova, J.; Jackson, M.; Mikusova, K.; Guerin, M.E.
    Structural basis for selective recognition of acyl chains by the membrane-associated acyltransferase PatA (2016), Nat. Commun., 7, 10906 .
    View publication on PubMedView publication on EuropePMC

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
2.3.1.265 using 100mM Tris-HCl pH 7.0, 230 mM MgCl2 and 12-16% (w/v) PEG 8000 Mycolicibacterium smegmatis

Engineering

EC Number Protein Variants Comment Organism
2.3.1.265 R164A the mutant shows about 78% activity compared to the wild type enzyme Mycolicibacterium smegmatis
2.3.1.265 H284A the mutant shows about 50% activity compared to the wild type enzyme Mycolicibacterium smegmatis
2.3.1.265 E149A the mutant shows about 75% activity compared to the wild type enzyme Mycolicibacterium smegmatis
2.3.1.265 E200A the mutant shows less than 3% activity compared to the wild type enzyme Mycolicibacterium smegmatis
2.3.1.265 H126A the mutant shows less than 3% activity compared to the wild type enzyme Mycolicibacterium smegmatis
2.3.1.265 D131A the mutant shows about 35% activity compared to the wild type enzyme Mycolicibacterium smegmatis
2.3.1.265 F182W/L197W the mutant shows less than 3% activity compared to the wild type enzyme Mycolicibacterium smegmatis

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
2.3.1.265 membrane
-
Mycolicibacterium smegmatis
-
-

Organism

EC Number Organism UniProt Comment Textmining
2.3.1.265 Mycolicibacterium smegmatis A0QWG5
-
-
2.3.1.265 Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 A0QWG5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
2.3.1.265 HisTrap column chromatography Mycolicibacterium smegmatis

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
2.3.1.265 palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
Mycolicibacterium smegmatis CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
?
2.3.1.265 palmitoyl-CoA + 2,6-di-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-6-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
?
2.3.1.265 palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
Mycolicibacterium smegmatis CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?
2.3.1.265 palmitoyl-CoA + 2-O-alpha-D-mannosyl-1-phosphatidyl-1D-myo-inositol
-
Mycolicibacterium smegmatis mc(2)155 / ATCC 700084 CoA + 2-O-(6-O-palmitoyl-alpha-D-mannosyl)-1-phosphatidyl-1D-myo-inositol
-
?

Synonyms

EC Number Synonyms Comment Organism
2.3.1.265 PatA
-
Mycolicibacterium smegmatis
2.3.1.265 Rv2611c
-
Mycolicibacterium smegmatis