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Literature summary extracted from

  • Jang, E.H.; Park, S.A.; Chi, Y.M.; Lee, K.S.
    Kinetic and structural characterization for cofactor preference of succinic semialdehyde dehydrogenase from Streptococcus pyogenes (2014), Mol. Cells, 37, 719-726 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.16 gene ssadh, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3) Streptococcus pyogenes

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.2.1.16 purified recombinant SpSSADH in apo-form and in a binary complex with NADP+, hanging drop vapor diffusion method, for the apoform crystals: mixing 0.001 ml of protein solution with 0.001 ml of reservoir solution containing 0.1 M MES monohydrate, pH 5.8-6.1, and 18-23% w/v PEG 4000, and equilibration over 0.5 ml reservoir solution, for the binary complex crystals: mixing of protein solution with NADP+ in a 1:10 molar ratio with reservoir solution containing 0.1 M sodium acetate trihydrate, pH 4.6, and 2 M ammonium sulfate, 22°C, X-ray diffraction structure determination and analysis at 1.6 A and 2.1 A resolutions, respectively, molecular replacement method using the structure of Escherichia coli SSADH, PDB ID 3JZ4, as search model Streptococcus pyogenes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.16 additional information
-
 additional information Michaelis-Menten kinetics, kinetic analysis Streptococcus pyogenes
1.2.1.16 0.1
-
 NADP+ recombinant His6-tagged enzyme, pH 7.0, 30°C Streptococcus pyogenes
1.2.1.16 0.23
-
 NAD+ recombinant His6-tagged enzyme, pH 7.0, 30°C Streptococcus pyogenes

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.16 succinate semialdehyde + NADP+ + H2O Streptococcus pyogenes
-
 succinate + NADPH + 2 H+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.16 Streptococcus pyogenes A0A0J9X1M8
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.16 recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Streptococcus pyogenes

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.16 succinate semialdehyde + NAD+ + H2O
-
 Streptococcus pyogenes succinate + NADH + 2 H+
-
 ?
1.2.1.16 succinate semialdehyde + NADP+ + H2O
-
 Streptococcus pyogenes succinate + NADPH + 2 H+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.16 homodimer enzyme SpSSADH shows a dimeric conformation, containing a single cysteine residue in the catalytic loop of each subunit Streptococcus pyogenes

Synonyms

EC Number Synonyms Comment Organism
1.2.1.16 SpSSADH
-
 Streptococcus pyogenes
1.2.1.16 SSADH
-
 Streptococcus pyogenes
1.2.1.16 succinic semialdehyde dehydrogenase
-
 Streptococcus pyogenes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.16 30
-
 assay at Streptococcus pyogenes

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.2.1.16 1.94
-
 NAD+ recombinant His6-tagged enzyme, pH 7.0, 30°C Streptococcus pyogenes
1.2.1.16 7.2
-
 NADP+ recombinant His6-tagged enzyme, pH 7.0, 30°C Streptococcus pyogenes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.16 7
-
 assay at Streptococcus pyogenes

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.16 NAD+
-
 Streptococcus pyogenes
1.2.1.16 NADP+ SpSSADH prefers NADP+ over NAD+ as a hydride acceptor, structural basis of the cofactor preference of SpSSADH, overview. Residues Ser158 and Tyr188 in SpSSADH participate in the stabilization of the 2'-phosphate group of adenine-side ribose in NADP+ Streptococcus pyogenes

General Information

EC Number General Information Comment Organism
1.2.1.16 metabolism succinic semialdehyde dehydrogenase (SSADH) catalyzes the oxidation of succinic semialdehyde to succinic acid in the final step of GABA catabolism Streptococcus pyogenes

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.2.1.16 9.7
-
 NAD+ recombinant His6-tagged enzyme, pH 7.0, 30°C Streptococcus pyogenes
1.2.1.16 72
-
 NADP+ recombinant His6-tagged enzyme, pH 7.0, 30°C Streptococcus pyogenes