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Literature summary extracted from

  • Kumar, S.; Jain, K.K.; Rani, S.; Bhardwaj, K.N.; Goel, M.; Kuhad, R.C.
    In-vitro refolding and characterization of recombinant laccase (CotA) from Bacillus pumilus MK001 and its potential for phenolics degradation (2016), Mol. Biotechnol., 58, 789-800 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 efficiently expressed in Escherichia coli BL21(DE3) and process is developed to harvest maximum potential of the enzyme by the refolding of the inclusion bodies Bacillus pumilus

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.10.3.2 0.1329
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 30°C Bacillus pumilus
1.10.3.2 7
-
 guaiacol pH 8.0, 30°C Bacillus pumilus

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Bacillus pumilus I3RYX9
-
-
1.10.3.2 Bacillus pumilus MK001 I3RYX9
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.10.3.2
-
 Bacillus pumilus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2
-
 Bacillus pumilus MK001 ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Bacillus pumilus ?
-
 ?
1.10.3.2 guaiacol + O2
-
 Bacillus pumilus MK001 ?
-
 ?
1.10.3.2 additional information a 3D model shows potential binding affinities with ferulic acid, caffeic acid, and vanillin Bacillus pumilus ?
-
 ?
1.10.3.2 additional information a 3D model shows potential binding affinities with ferulic acid, caffeic acid, and vanillin Bacillus pumilus MK001 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.10.3.2 ? x * 58880, calculation from sequence Bacillus pumilus

Synonyms

EC Number Synonyms Comment Organism
1.10.3.2 CotA
-
 Bacillus pumilus
1.10.3.2 spore coat A protein
-
 Bacillus pumilus

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 60
-
 substrate: guaiacol Bacillus pumilus
1.10.3.2 80
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus pumilus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 30
-
 24 h, 10% loss of activity Bacillus pumilus
1.10.3.2 60
-
 360 min, 40% loss of activity Bacillus pumilus
1.10.3.2 80
-
 90 min, 25% loss of activity Bacillus pumilus
1.10.3.2 90
-
 35 min, 30% loss of activity Bacillus pumilus

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.10.3.2 40
-
 guaiacol pH 8.0, 30°C Bacillus pumilus
1.10.3.2 377
-
 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) pH 5.0, 30°C Bacillus pumilus

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 5
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus pumilus
1.10.3.2 8
-
 substrate: guaiacol Bacillus pumilus

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.10.3.2 5
-
 24 h, 25% loss of activity Bacillus pumilus
1.10.3.2 9
-
 24 h, 15% loss of activity Bacillus pumilus
1.10.3.2 11
-
 24 h, 40% loss of activity Bacillus pumilus

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.10.3.2 Bacillus pumilus calculation from sequence
-
 5.8