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Literature summary extracted from

  • Man, Z.; Rao, Z.; Xu, M.; Guo, J.; Yang, T.; Zhang, X.; Xu, Z.
    Improvement of the intracellular environment for enhancing L-arginine production of Corynebacterium glutamicum by inactivation of H2O2-forming flavin reductases and optimization of ATP supply (2016), Metab. Eng., 38, 310-321 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.1.30 gene frd2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Corynebacterium crenatum
1.5.1.36 gene frd2, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Corynebacterium crenatum
1.5.1.41 gene frd1, DNA and amino acid sequence determination and analysis, sequence comparisons, recombinant overexpression of N-terminally His-tagged enzyme in Escherichia coli strain BL21(DE3) Corynebacterium crenatum

Protein Variants

EC Number Protein Variants Comment Organism
1.5.1.30 additional information improvement of the intracellular environment for enhancing L-arginine production of Corynebacterium glutamicum by inactivation of H2O2-forming flavin reductases and optimization of ATP supply. Construction of mutants of gene frd2, strain 5-5(frd2) and deletion strains 5-5DELTAfrd2 and 5-5DELTAfrd12. The extracellular H2O2 concentrations of mutant 5-5DELTAfrd12 are lower than that of the wild-type strain SYPA5-5, and the extracellular H2O2 concentrations of mutant 5-5(frd2) is increased compared to the wild-type. Flavin reductase activities in frd1 and frd2 overexpression and deletion strains with NADH and FAD, overview Corynebacterium crenatum
1.5.1.36 additional information improvement of the intracellular environment for enhancing L-arginine production of Corynebacterium glutamicum by inactivation of H2O2-forming flavin reductases and optimization of ATP supply. Construction of mutants of gene frd2, strain 5-5(frd2) and deletion strains 5-5DELTAfrd2 and 5-5DELTAfrd12. The extracellular H2O2 concentrations of mutant 5-5DELTAfrd12 are lower than that of the wild-type strain SYPA5-5, and the extracellular H2O2 concentrations of mutant 5-5(frd2) is increased compared to the wild-type. Flavin reductase activities in frd1 and frd2 overexpression and deletion strains with NADH and FAD, overview Corynebacterium crenatum
1.5.1.41 additional information improvement of the intracellular environment for enhancing L-arginine production of Corynebacterium glutamicum by inactivation of H2O2-forming flavin reductases and optimization of ATP supply. Construction of mutants of gene frd1, strain 5-5(frd1) and deletion strains 5-5DELTAfrd1 and 5-5DELTAfrd12. The extracellular H2O2 concentrations of mutants 5-5DELTAfrd1 and 5-5DELTAfrd12 are lower than that of the wild-type strain SYPA5-5, and the extracellular H2O2 concentrations of mutant 5-5(frd1) is increased compared to the wild-type. Flavin reductase activities in frd1 and frd2 overexpression and deletion strains with NADH and FAD, overview Corynebacterium crenatum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.5.1.30 0.0109
-
 NADPH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.30 0.0184
-
 NADPH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.36 0.0084
-
 NADH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.36 0.0141
-
 NADH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.0044
-
 NADH with riboflavin, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.0052
-
 NADH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.0056
-
 NADH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.0068
-
 NADPH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.0085
-
 NADPH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.5.1.30 FAD + NAD(P)H Corynebacterium crenatum
-
 FADH2 + NAD(P)+
-
 r
1.5.1.30 FAD + NAD(P)H Corynebacterium crenatum SYPA5-5
-
 FADH2 + NAD(P)+
-
 r
1.5.1.30 FMN + NAD(P)H Corynebacterium crenatum
-
 FMNH2 + NAD(P)+
-
 r
1.5.1.30 FMN + NAD(P)H Corynebacterium crenatum SYPA5-5
-
 FMNH2 + NAD(P)+
-
 r
1.5.1.36 FAD + NAD(P)H Corynebacterium crenatum
-
 FADH2 + NAD(P)+
-
 r
1.5.1.36 FMN + NAD(P)H Corynebacterium crenatum
-
 FMNH2 + NAD(P)+
-
 r
1.5.1.41 FAD + NAD(P)H Corynebacterium crenatum
-
 FADH2 + NAD(P)+
-
 r
1.5.1.41 FMN + NAD(P)H Corynebacterium crenatum
-
 FMNH2 + NAD(P)+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.5.1.30 Corynebacterium crenatum
-
-
-
1.5.1.30 Corynebacterium crenatum SYPA5-5
-
-
-
1.5.1.36 Corynebacterium crenatum
-
-
-
1.5.1.36 Corynebacterium crenatum SYPA5-5
-
-
-
1.5.1.41 Corynebacterium crenatum
-
-
-
1.5.1.41 Corynebacterium crenatum SYPA5-5
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.1.30 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Corynebacterium crenatum
1.5.1.36 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Corynebacterium crenatum
1.5.1.41 recombinant N-terminally His-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography Corynebacterium crenatum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.1.30 FAD + NAD(P)H
-
 Corynebacterium crenatum FADH2 + NAD(P)+
-
 r
1.5.1.30 FAD + NAD(P)H
-
 Corynebacterium crenatum SYPA5-5 FADH2 + NAD(P)+
-
 r
1.5.1.30 FAD + NADPH
-
 Corynebacterium crenatum FADH2 + NADP+
-
 r
1.5.1.30 FAD + NADPH
-
 Corynebacterium crenatum SYPA5-5 FADH2 + NADP+
-
 r
1.5.1.30 FMN + NAD(P)H
-
 Corynebacterium crenatum FMNH2 + NAD(P)+
-
 r
1.5.1.30 FMN + NAD(P)H
-
 Corynebacterium crenatum SYPA5-5 FMNH2 + NAD(P)+
-
 r
1.5.1.30 additional information the enzyme is also active with FMN and NADH, cf. EC 1.5.1.36 Corynebacterium crenatum ?
-
 ?
1.5.1.30 additional information the enzyme is also active with FMN and NADH, cf. EC 1.5.1.36 Corynebacterium crenatum SYPA5-5 ?
-
 ?
1.5.1.36 FAD + NAD(P)H
-
 Corynebacterium crenatum FADH2 + NAD(P)+
-
 r
1.5.1.36 FMN + NAD(P)H
-
 Corynebacterium crenatum FMNH2 + NAD(P)+
-
 r
1.5.1.36 FMN + NADH
-
 Corynebacterium crenatum FMNH2 + NAD+
-
 r
1.5.1.36 FMN + NADPH
-
 Corynebacterium crenatum FMNH2 + NADP+
-
 r
1.5.1.36 additional information the enzyme is also active with FMN and NADPH, cf. EC 1.5.1.30 Corynebacterium crenatum ?
-
 ?
1.5.1.36 additional information the enzyme is also active with FMN and NADPH, cf. EC 1.5.1.30 Corynebacterium crenatum SYPA5-5 ?
-
 ?
1.5.1.41 FAD + NAD(P)H
-
 Corynebacterium crenatum FADH2 + NAD(P)+
-
 r
1.5.1.41 FAD + NADPH
-
 Corynebacterium crenatum FADH2 + NADP+
-
 r
1.5.1.41 FMN + NAD(P)H
-
 Corynebacterium crenatum FMNH2 + NAD(P)+
-
 r
1.5.1.41 FMN + NADH
-
 Corynebacterium crenatum FMNH2 + NAD+
-
 r
1.5.1.41 FMN + NADPH
-
 Corynebacterium crenatum FMNH2 + NADP+
-
 r
1.5.1.41 riboflavin + NADH preferred substrate Corynebacterium crenatum FMNH2 + NAD+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.5.1.30 Frd188
-
 Corynebacterium crenatum
1.5.1.30 frd2
-
 Corynebacterium crenatum
1.5.1.30 NAD(P)H-dependent H2O2-forming flavin reductase
-
 Corynebacterium crenatum
1.5.1.36 Frd188
-
 Corynebacterium crenatum
1.5.1.36 frd2
-
 Corynebacterium crenatum
1.5.1.36 NAD(P)H-dependent H2O2-forming flavin reductase
-
 Corynebacterium crenatum
1.5.1.41 frd1
-
 Corynebacterium crenatum
1.5.1.41 Frd181
-
 Corynebacterium crenatum
1.5.1.41 NAD(P)H-dependent H2O2-forming flavin reductase
-
 Corynebacterium crenatum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.1.30 30
-
 assay at Corynebacterium crenatum
1.5.1.36 30
-
 assay at Corynebacterium crenatum
1.5.1.41 30
-
 assay at Corynebacterium crenatum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.5.1.30 0.0282
-
 NADPH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.30 0.036
-
 NADPH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.36 0.0247
-
 NADH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.36 0.0357
-
 NADH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.061
-
 NADPH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.069
-
 NADPH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.09
-
 NADH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.547
-
 NADH with riboflavin, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 0.603
-
 NADH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.5.1.30 7.5
-
 assay at Corynebacterium crenatum
1.5.1.36 7.5
-
 assay at Corynebacterium crenatum
1.5.1.41 7.5
-
 assay at Corynebacterium crenatum

Cofactor

EC Number Cofactor Comment Organism Structure
1.5.1.30 NADP+
-
 Corynebacterium crenatum
1.5.1.30 NADPH
-
 Corynebacterium crenatum
1.5.1.36 NAD+
-
 Corynebacterium crenatum
1.5.1.36 NADH
-
 Corynebacterium crenatum
1.5.1.41 NAD+
-
 Corynebacterium crenatum
1.5.1.41 NADH
-
 Corynebacterium crenatum
1.5.1.41 NADP+
-
 Corynebacterium crenatum
1.5.1.41 NADPH
-
 Corynebacterium crenatum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.5.1.30 1.96
-
 NADPH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.30 2.59
-
 NADPH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.36 2.53
-
 NADH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.36 2.94
-
 NADH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 7.18
-
 NADPH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 10.15
-
 NADPH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 17.31
-
 NADH with FMN, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 107.68
-
 NADH with FAD, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum
1.5.1.41 136.75
-
 NADH with riboflavin, pH 7.5, 30°C, recombinant enzyme Corynebacterium crenatum