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Literature summary extracted from

  • Tong, Y.; Xin, Y.; Yang, H.; Zhang, L.; Tao, X.; Xu, H.; Wang, W.
    Novel affinity purification of monomeric sarcosine oxidase expressed in Escherichia coli (2013), J. Sep. Sci., 36, 3086-3092 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.5.3.1 expression in Escherichia coli Bacillus sp. BSD-8

Organism

EC Number Organism UniProt Comment Textmining
1.5.3.1 Bacillus sp. BSD-8 Q6ITC6
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.5.3.1 affinity-purification, 4-aminopyrrole-2-carboxylic acid as the affinity ligand, which is coupled with Sepharose CL 4B via spacers composed of epichlorohydrin and ethylenediamine. With the affinity medium, the purification process consists of only one affinity chromatography step to capture monomeric enzyme. The purified enzyme iss 94% and 96% pure when analyzed on an HPLC and reducing SDS-PAGE Bacillus sp. BSD-8

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.5.3.1 sarcosine + H2O + O2
-
 Bacillus sp. BSD-8 glycine + formaldehyde + H2O2
-
 ?

Subunits

EC Number Subunits Comment Organism
1.5.3.1 monomer 1 * 46000, SDS-PAGE Bacillus sp. BSD-8

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.5.3.1 37
-
 assay at Bacillus sp. BSD-8