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Literature summary extracted from

  • Han, B.; Liu, S.; Gai, Y.; Jiang, X.
    Preparation of cinnamoyl-CoA and analysis of the enzyme activity of recombinant CCR protein from Populus tomentosa (2014), J. Plant Biochem. Biotechnol., 23, 348-357 .
No PubMed abstract available

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.2.1.44 CCR, recombinant expression of His-tagged enzyme CCR in Escherichia coli, coexpression with 4-coumarate-CoA ligase, EC 6.2.1.12. The best expression conditions for recombinant PtCCR are 6 h of 0.4 mM IPTG induction at 37°C Populus tomentosa

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.2.1.44 additional information
-
 additional information Michaelis-Menten kinetics analysis Populus tomentosa
1.2.1.44 0.323
-
 feruloyl-CoA pH 6.25, 37°C, recombinant His-tagged enzyme Populus tomentosa
1.2.1.44 0.377
-
 4-coumaroyl-CoA pH 6.25, 37°C, recombinant His-tagged enzyme Populus tomentosa
1.2.1.44 0.617
-
 caffeoyl-CoA pH 6.25, 37°C, recombinant His-tagged enzyme Populus tomentosa

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.44 4-coumaroyl-CoA + NADPH + H+ Populus tomentosa
-
 4-coumaraldehyde + CoA + NADP+
-
 ?
1.2.1.44 caffeoyl-CoA + NADPH + H+ Populus tomentosa
-
 caffealdehyde + CoA + NADP+
-
 ?
1.2.1.44 cinnamoyl-CoA + NADPH + H+ Populus tomentosa
-
 cinnamaldehyde + CoA + NADP+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH + H+ Populus tomentosa preferred substrate coniferaldehyde + CoA + NADP+
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH + H+ Populus tomentosa
-
 sinapaldehyde + CoA + NADP+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Populus tomentosa Q6S3D7
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.2.1.44 recombinant His-tagged enzyme CCR from Escherichia coli by nickel affinity chromatography and gel filtration Populus tomentosa

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 4-coumaroyl-CoA + NADPH + H+
-
 Populus tomentosa 4-coumaraldehyde + CoA + NADP+
-
 ?
1.2.1.44 caffeoyl-CoA + NADPH + H+
-
 Populus tomentosa caffealdehyde + CoA + NADP+
-
 ?
1.2.1.44 cinnamoyl-CoA + NADPH + H+
-
 Populus tomentosa cinnamaldehyde + CoA + NADP+
-
 ?
1.2.1.44 feruloyl-CoA + NADPH + H+ preferred substrate Populus tomentosa coniferaldehyde + CoA + NADP+
-
 ?
1.2.1.44 additional information the substrates of CCR, cinnamoyl-CoA esters, are products of 4-coumarate-CoA ligase (4CL, EC 6.2.1.12), which is an enzyme upstream of CCR. Recombinant Pt4CL can catalyze the conversion of hydroxycinnamic acids to cinnamoyl-CoA esters, with high efficiency Populus tomentosa ?
-
 ?
1.2.1.44 sinapoyl-CoA + NADPH + H+
-
 Populus tomentosa sinapaldehyde + CoA + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.2.1.44 ? x * 37000, recombinant His-tagged enzyme CCR, SDS-PAGE Populus tomentosa

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 CCR
-
 Populus tomentosa

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.2.1.44 37
-
 assay at Populus tomentosa

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.2.1.44 6.25
-
 assay at Populus tomentosa

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.2.1.44 5.5 6.9 activity range, inactive above pH 7.0, profile overview Populus tomentosa

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.44 NADPH
-
 Populus tomentosa

General Information

EC Number General Information Comment Organism
1.2.1.44 physiological function cinnamoyl-CoA reductase catalyzes the reduction of cinnamoyl-CoA esters to their respective cinnamaldehydes and is considered as a key enzyme in lignin formation Populus tomentosa