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Literature summary extracted from

  • Boyd, D.R.; Sharma, N.D.; Malone, J.F.; McIntyre, P.B.; McRoberts, C.; Floyd, S.; Allen, C.C.; Gohil, A.; Coles, S.J.; Horton, P.N.; Stevenson, P.J.
    Toluene dioxygenase-catalyzed synthesis and reactions of cis-diol metabolites derived from 2- and 3-methoxyphenols (2015), J. Org. Chem., 80, 3429-3439 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.12.11 expressed in Escherichia coli BL21(DE3) cells Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.12.11 toluene + NADH + H+ + O2 Pseudomonas putida
-
 (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.11 toluene + NADH + H+ + O2 Pseudomonas putida UV4
-
 (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.12.11 Pseudomonas putida
-
-
-
1.14.12.11 Pseudomonas putida UV4
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.12.11 (4S,5S,6S)-4,5-dihydroxy-6-methoxycyclohex-2-enone + NADH + H+ + O2
-
 Pseudomonas putida (2S,3S,4S)-3,4-dihydroxy-2-methoxycyclohexanone + NAD+
-
 ?
1.14.12.11 (4S,5S,6S)-4,5-dihydroxy-6-methoxycyclohex-2-enone + NADH + H+ + O2
-
 Pseudomonas putida UV4 (2S,3S,4S)-3,4-dihydroxy-2-methoxycyclohexanone + NAD+
-
 ?
1.14.12.11 2-methoxyphenol + NADH + H+ + O2
-
 Pseudomonas putida (4S,5S)-4,5-dihydroxy-2-methoxycyclohex-2-enone + NAD+
-
 ?
1.14.12.11 2-methoxyphenol + NADH + H+ + O2
-
 Pseudomonas putida UV4 (4S,5S)-4,5-dihydroxy-2-methoxycyclohex-2-enone + NAD+
-
 ?
1.14.12.11 3-methoxyphenol + NADH + H+ + O2
-
 Pseudomonas putida (4S,5S)-4,5-dihydroxy-3-methoxycyclohex-2-enone + NAD+
-
 ?
1.14.12.11 3-methoxyphenol + NADH + H+ + O2
-
 Pseudomonas putida UV4 (4S,5S)-4,5-dihydroxy-3-methoxycyclohex-2-enone + NAD+
-
 ?
1.14.12.11 toluene + NADH + H+ + O2
-
 Pseudomonas putida (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?
1.14.12.11 toluene + NADH + H+ + O2
-
 Pseudomonas putida UV4 (1S,2R)-3-methylcyclohexa-3,5-diene-1,2-diol + NAD+
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.14.12.11 TDO
-
 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.12.11 NADH
-
 Pseudomonas putida