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Literature summary extracted from

  • Loncar, N.; Bozic, N.; Vujcic, Z.
    Expression and characterization of a thermostable organic solvent-tolerant laccase from Bacillus licheniformis ATCC 9945a (2016), J. Mol. Catal. B, 134, 390-395 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.10.3.2 EDTA 1 mM, 1.27fold activation Bacillus licheniformis

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.10.3.2 heterologously expressed in Escherichia coli Bacillus licheniformis

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.10.3.2 dithiothreitol 1 mM, complete inactivation Bacillus licheniformis
1.10.3.2 L-cysteine 1 mM, complete inactivation Bacillus licheniformis
1.10.3.2 NaN3 1 mM, 60% inhibition Bacillus licheniformis

Organic Solvent Stability

EC Number Organic Solvent Comment Organism
1.10.3.2 acetonitrile presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired Bacillus licheniformis
1.10.3.2 dimethyl formamide presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired Bacillus licheniformis
1.10.3.2 dimethyl sulfoxide presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired Bacillus licheniformis
1.10.3.2 Methanol presence of 10% organic solvent reduces melting temperature from 79°C to to 45-52°C but activity remains practically unimpaired Bacillus licheniformis

Organism

EC Number Organism UniProt Comment Textmining
1.10.3.2 Bacillus licheniformis
-
-
-
1.10.3.2 Bacillus licheniformis ATCC 9945a
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.10.3.2 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 73% of the activity compared to syringaldazine Bacillus licheniformis ?
-
 ?
1.10.3.2 2,6-dimethoxyphenol + O2 69% of the activity compared to syringaldazine Bacillus licheniformis ?
-
 ?
1.10.3.2 catechol + O2 28% of the activity compared to syringaldazine Bacillus licheniformis ?
-
 ?
1.10.3.2 catechol + O2 28% of the activity compared to syringaldazine Bacillus licheniformis ATCC 9945a ?
-
 ?
1.10.3.2 gallic acid + O2 45% of the activity compared to syringaldazine Bacillus licheniformis ?
-
 ?
1.10.3.2 gallic acid + O2 45% of the activity compared to syringaldazine Bacillus licheniformis ATCC 9945a ?
-
 ?
1.10.3.2 L-DOPA + O2 35% of the activity compared to syringaldazine Bacillus licheniformis ?
-
 ?
1.10.3.2 additional information the enzyme mediates C-C bond formation via oxidative coupling after one electron oxidation of phenolic group. Successful polymerization of 2-naphthol is achieved with 77% conversion of 250 mg/l 2-naphtol in 15 min Bacillus licheniformis ?
-
 ?
1.10.3.2 additional information the enzyme mediates C-C bond formation via oxidative coupling after one electron oxidation of phenolic group. Successful polymerization of 2-naphthol is achieved with 77% conversion of 250 mg/l 2-naphtol in 15 min Bacillus licheniformis ATCC 9945a ?
-
 ?
1.10.3.2 o-dianisidine + O2 90% of the activity compared to syringaldazine Bacillus licheniformis ?
-
 ?
1.10.3.2 p-phenylenediamine + O2 107% of the activity compared to syringaldazine Bacillus licheniformis ?
-
 ?
1.10.3.2 p-phenylenediamine + O2 107% of the activity compared to syringaldazine Bacillus licheniformis ATCC 9945a ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Bacillus licheniformis ?
-
 ?
1.10.3.2 syringaldazine + O2
-
 Bacillus licheniformis ATCC 9945a ?
-
 ?

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.10.3.2 90
-
-
 Bacillus licheniformis

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.10.3.2 45 52 melting temperature in presence of 10% organic solvents such as acetonitrile, dimethylformamide, dimethylsulfoxide or methanol Bacillus licheniformis
1.10.3.2 70
-
 half-life: 50 min Bacillus licheniformis
1.10.3.2 79
-
 melting temperature at pH 7.0. Presence of 10% organic solvents such as acetonitrile, dimethylformamide, dimethylsulfoxide or methanol reduces melting temperature to 45-52°C Bacillus licheniformis

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.10.3.2 3
-
 substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) Bacillus licheniformis
1.10.3.2 7
-
 substrate: syringaldazine Bacillus licheniformis