BRENDA - Enzyme Database

Biochemical properties of a new nitrile reductase cloned from Pectobacterium carotovorum

Li, M.; Zhou, Z.; Zhang, Z.; Yu, H.; Xu, J.; J. Mol. Catal. B 131, 47-54 (2016)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.7.1.13
expression in Escherichia coli
Pectobacterium carotovorum subsp. carotovorum
Crystallization (Commentary)
EC Number
Crystallization (Commentary)
Organism
1.7.1.13
homology model based on the crystal structure of nitrile reductase from Vibrio cholerae, PDB ID 3uxv. The side chain of residue Glu89 and the main chain of Ser90 form hydrogen bonds with the ring nitrogen atoms of the substrate. The carboxylate group of Glu230 also forms hydrogen bonds with the substrate preQ0. The aromatic ring of Phe228 may form a pi-pi-stacking interaction with the aromatic ring of preQ0, while the main chain of His229coordinates the keto-functionality of the substrate
Pectobacterium carotovorum subsp. carotovorum
Inhibitors
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.13
EDTA
0.1 mM, 89% residual activity
Pectobacterium carotovorum subsp. carotovorum
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.1.13
0.00025
-
7-cyano-7-carbaguanine
below 0.25 microM, pH 7.4, 30°C
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
0.229
-
NADPH
pH 7.4, 30°C
Pectobacterium carotovorum subsp. carotovorum
Metals/Ions
EC Number
Metals/Ions
Commentary
Organism
Structure
1.7.1.13
Cu2+
0.01 mM, 82% residual activity
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
Fe3+
0.01 mM, 92% residual activity
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
Ni2+
0.01 mM, 77% residual activity
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
Zn2+
0.01 mM, 13.5% residual activity
Pectobacterium carotovorum subsp. carotovorum
Molecular Weight [Da]
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.1.13
62000
-
gel filtration
Pectobacterium carotovorum subsp. carotovorum
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.7.1.13
Pectobacterium carotovorum subsp. carotovorum
C6DAH4
-
-
1.7.1.13
Pectobacterium carotovorum subsp. carotovorum PCC21
C6DAH4
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.7.1.13
7-cyano-7-carbaguanine + 2 NADPH + 2 H+
-
743107
Pectobacterium carotovorum subsp. carotovorum
7-aminomethyl-7-carbaguanine + 2 NADP+
-
-
-
?
1.7.1.13
7-cyano-7-carbaguanine + 2 NADPH + 2 H+
-
743107
Pectobacterium carotovorum subsp. carotovorum PCC21
7-aminomethyl-7-carbaguanine + 2 NADP+
-
-
-
?
1.7.1.13
additional information
enzyme is highly specific for substrate preQ0
743107
Pectobacterium carotovorum subsp. carotovorum
?
-
-
-
?
1.7.1.13
additional information
enzyme is highly specific for substrate preQ0
743107
Pectobacterium carotovorum subsp. carotovorum PCC21
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.7.1.13
dimer
2 * 32000, SDS-PAGE
Pectobacterium carotovorum subsp. carotovorum
Synonyms
EC Number
Synonyms
Commentary
Organism
1.7.1.13
nitrile reductase
-
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
NRed
-
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
QueF
-
Pectobacterium carotovorum subsp. carotovorum
Temperature Optimum [°C]
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.7.1.13
40
-
-
Pectobacterium carotovorum subsp. carotovorum
Temperature Range [°C]
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.7.1.13
50
-
more than 90% of maximum activity
Pectobacterium carotovorum subsp. carotovorum
Temperature Stability [°C]
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.7.1.13
30
-
half-life 47.2 h
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
40
-
half-life 13.6 h
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
50
-
half-life 3.9 h
Pectobacterium carotovorum subsp. carotovorum
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7.1.13
0.024
-
7-cyano-7-carbaguanine
pH 7.4, 30°C
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
0.025
-
NADPH
pH 7.4, 30°C
Pectobacterium carotovorum subsp. carotovorum
pH Optimum
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.1.13
7.4
-
-
Pectobacterium carotovorum subsp. carotovorum
pH Range
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.7.1.13
6.5
8
more than 60% of maximum activity
Pectobacterium carotovorum subsp. carotovorum
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.13
NADPH
-
Pectobacterium carotovorum subsp. carotovorum
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.7.1.13
expression in Escherichia coli
Pectobacterium carotovorum subsp. carotovorum
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.7.1.13
NADPH
-
Pectobacterium carotovorum subsp. carotovorum
Crystallization (Commentary) (protein specific)
EC Number
Crystallization
Organism
1.7.1.13
homology model based on the crystal structure of nitrile reductase from Vibrio cholerae, PDB ID 3uxv. The side chain of residue Glu89 and the main chain of Ser90 form hydrogen bonds with the ring nitrogen atoms of the substrate. The carboxylate group of Glu230 also forms hydrogen bonds with the substrate preQ0. The aromatic ring of Phe228 may form a pi-pi-stacking interaction with the aromatic ring of preQ0, while the main chain of His229coordinates the keto-functionality of the substrate
Pectobacterium carotovorum subsp. carotovorum
Inhibitors (protein specific)
EC Number
Inhibitors
Commentary
Organism
Structure
1.7.1.13
EDTA
0.1 mM, 89% residual activity
Pectobacterium carotovorum subsp. carotovorum
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.7.1.13
0.00025
-
7-cyano-7-carbaguanine
below 0.25 microM, pH 7.4, 30°C
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
0.229
-
NADPH
pH 7.4, 30°C
Pectobacterium carotovorum subsp. carotovorum
Metals/Ions (protein specific)
EC Number
Metals/Ions
Commentary
Organism
Structure
1.7.1.13
Cu2+
0.01 mM, 82% residual activity
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
Fe3+
0.01 mM, 92% residual activity
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
Ni2+
0.01 mM, 77% residual activity
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
Zn2+
0.01 mM, 13.5% residual activity
Pectobacterium carotovorum subsp. carotovorum
Molecular Weight [Da] (protein specific)
EC Number
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
1.7.1.13
62000
-
gel filtration
Pectobacterium carotovorum subsp. carotovorum
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.7.1.13
7-cyano-7-carbaguanine + 2 NADPH + 2 H+
-
743107
Pectobacterium carotovorum subsp. carotovorum
7-aminomethyl-7-carbaguanine + 2 NADP+
-
-
-
?
1.7.1.13
7-cyano-7-carbaguanine + 2 NADPH + 2 H+
-
743107
Pectobacterium carotovorum subsp. carotovorum PCC21
7-aminomethyl-7-carbaguanine + 2 NADP+
-
-
-
?
1.7.1.13
additional information
enzyme is highly specific for substrate preQ0
743107
Pectobacterium carotovorum subsp. carotovorum
?
-
-
-
?
1.7.1.13
additional information
enzyme is highly specific for substrate preQ0
743107
Pectobacterium carotovorum subsp. carotovorum PCC21
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.7.1.13
dimer
2 * 32000, SDS-PAGE
Pectobacterium carotovorum subsp. carotovorum
Temperature Optimum [°C] (protein specific)
EC Number
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
1.7.1.13
40
-
-
Pectobacterium carotovorum subsp. carotovorum
Temperature Range [°C] (protein specific)
EC Number
Temperature Minimum [°C]
Temperature Maximum [°C]
Commentary
Organism
1.7.1.13
50
-
more than 90% of maximum activity
Pectobacterium carotovorum subsp. carotovorum
Temperature Stability [°C] (protein specific)
EC Number
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
1.7.1.13
30
-
half-life 47.2 h
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
40
-
half-life 13.6 h
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
50
-
half-life 3.9 h
Pectobacterium carotovorum subsp. carotovorum
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.7.1.13
0.024
-
7-cyano-7-carbaguanine
pH 7.4, 30°C
Pectobacterium carotovorum subsp. carotovorum
1.7.1.13
0.025
-
NADPH
pH 7.4, 30°C
Pectobacterium carotovorum subsp. carotovorum
pH Optimum (protein specific)
EC Number
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
1.7.1.13
7.4
-
-
Pectobacterium carotovorum subsp. carotovorum
pH Range (protein specific)
EC Number
pH Minimum
pH Maximum
Commentary
Organism
1.7.1.13
6.5
8
more than 60% of maximum activity
Pectobacterium carotovorum subsp. carotovorum