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Literature summary extracted from
- Biochemical properties of a new nitrile reductase cloned from Pectobacterium carotovorum (2016), J. Mol. Catal. B, 131, 47-54 .
No PubMed abstract available
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.7.1.13 | expression in Escherichia coli | Pectobacterium carotovorum subsp. carotovorum |
Crystallization (Commentary)
| EC Number | Crystallization (Comment) | Organism |
|---|---|---|
| 1.7.1.13 | homology model based on the crystal structure of nitrile reductase from Vibrio cholerae, PDB ID 3uxv. The side chain of residue Glu89 and the main chain of Ser90 form hydrogen bonds with the ring nitrogen atoms of the substrate. The carboxylate group of Glu230 also forms hydrogen bonds with the substrate preQ0. The aromatic ring of Phe228 may form a pi-pi-stacking interaction with the aromatic ring of preQ0, while the main chain of His229coordinates the keto-functionality of the substrate | Pectobacterium carotovorum subsp. carotovorum |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.1.13 | EDTA | 0.1 mM, 89% residual activity | Pectobacterium carotovorum subsp. carotovorum |  |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.7.1.13 | 0.00025 | - |
7-cyano-7-carbaguanine | below 0.25 microM, pH 7.4, 30°C | Pectobacterium carotovorum subsp. carotovorum | |
| 1.7.1.13 | 0.229 | - |
NADPH | pH 7.4, 30°C | Pectobacterium carotovorum subsp. carotovorum | |
Metals/Ions
| EC Number | Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.1.13 | Cu2+ | 0.01 mM, 82% residual activity | Pectobacterium carotovorum subsp. carotovorum | |
| 1.7.1.13 | Fe3+ | 0.01 mM, 92% residual activity | Pectobacterium carotovorum subsp. carotovorum | |
| 1.7.1.13 | Ni2+ | 0.01 mM, 77% residual activity | Pectobacterium carotovorum subsp. carotovorum | |
| 1.7.1.13 | Zn2+ | 0.01 mM, 13.5% residual activity | Pectobacterium carotovorum subsp. carotovorum | |
Molecular Weight [Da]
| EC Number | Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.13 | 62000 | - |
gel filtration | Pectobacterium carotovorum subsp. carotovorum |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.7.1.13 | Pectobacterium carotovorum subsp. carotovorum | C6DAH4 | - |
- |
| 1.7.1.13 | Pectobacterium carotovorum subsp. carotovorum PCC21 | C6DAH4 | - |
- |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.7.1.13 | 7-cyano-7-carbaguanine + 2 NADPH + 2 H+ | - |
Pectobacterium carotovorum subsp. carotovorum | 7-aminomethyl-7-carbaguanine + 2 NADP+ | - |
? | |
| 1.7.1.13 | 7-cyano-7-carbaguanine + 2 NADPH + 2 H+ | - |
Pectobacterium carotovorum subsp. carotovorum PCC21 | 7-aminomethyl-7-carbaguanine + 2 NADP+ | - |
? | |
| 1.7.1.13 | additional information | enzyme is highly specific for substrate preQ0 | Pectobacterium carotovorum subsp. carotovorum | ? | - |
? | |
| 1.7.1.13 | additional information | enzyme is highly specific for substrate preQ0 | Pectobacterium carotovorum subsp. carotovorum PCC21 | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.7.1.13 | dimer | 2 * 32000, SDS-PAGE | Pectobacterium carotovorum subsp. carotovorum |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.7.1.13 | nitrile reductase | - |
Pectobacterium carotovorum subsp. carotovorum |
| 1.7.1.13 | NRed | - |
Pectobacterium carotovorum subsp. carotovorum |
| 1.7.1.13 | QueF | - |
Pectobacterium carotovorum subsp. carotovorum |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.13 | 40 | - |
- |
Pectobacterium carotovorum subsp. carotovorum |
Temperature Range [°C]
| EC Number | Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.13 | 50 | - |
more than 90% of maximum activity | Pectobacterium carotovorum subsp. carotovorum |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.13 | 30 | - |
half-life 47.2 h | Pectobacterium carotovorum subsp. carotovorum |
| 1.7.1.13 | 40 | - |
half-life 13.6 h | Pectobacterium carotovorum subsp. carotovorum |
| 1.7.1.13 | 50 | - |
half-life 3.9 h | Pectobacterium carotovorum subsp. carotovorum |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.7.1.13 | 0.024 | - |
7-cyano-7-carbaguanine | pH 7.4, 30°C | Pectobacterium carotovorum subsp. carotovorum | |
| 1.7.1.13 | 0.025 | - |
NADPH | pH 7.4, 30°C | Pectobacterium carotovorum subsp. carotovorum | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.13 | 7.4 | - |
- |
Pectobacterium carotovorum subsp. carotovorum |
pH Range
| EC Number | pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.7.1.13 | 6.5 | 8 | more than 60% of maximum activity | Pectobacterium carotovorum subsp. carotovorum |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.7.1.13 | NADPH | - |
Pectobacterium carotovorum subsp. carotovorum | |
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Release 2023.1 (January 2023)
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