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Literature summary extracted from

  • Wang, J.; Feng, J.; Li, W.; Yang, C.; Chen, X.; Bao, B.; Yang, J.; Wang, P.; Li, D.; Shi, R.
    Characterization of a novel (R)-mandelate dehydrogenase from Pseudomonas putida NUST506 (2015), J. Mol. Catal. B, 120, 23-27 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
1.1.1.B65 DTT enhancement of enzyme activity of 26% and 33% in the concentration of 10 mM and 50 mM Pseudomonas putida
1.1.1.B65 Triton X-100 0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity Pseudomonas putida

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.B65 Ca2+ inhibits 4% aT 1 mM and 33% at 10 mM Pseudomonas putida
1.1.1.B65 EDTA inhibits the enzyme activity about 68% and 89% in the concentration of 10 mM and 50 mM,respectively Pseudomonas putida
1.1.1.B65 Hg2+ strong inhibition at 1 mM Pseudomonas putida
1.1.1.B65 Li+ inhibits 17% at 10 mM Pseudomonas putida
1.1.1.B65 Mg2+ inhibits 19% at 1 mM and 31% at 10 mM Pseudomonas putida
1.1.1.B65 Mn2+ 21% activation at 1 mM, and 61% inhibition at 10 mM Pseudomonas putida
1.1.1.B65 Ni2+ inhibits 39% at 1 mM and 63% at 10 mM Pseudomonas putida
1.1.1.B65 SDS strongly inhibits the enzyme activity at 0.25-0.75% Pseudomonas putida
1.1.1.B65 Triton X-100 0.25% of TritonX-100 has slight improvement on enzyme activity, whereas 0.75% of TritonX-100 slightly inhibits the enzyme activity Pseudomonas putida
1.1.1.B65 Zn2+ strong inhibition at 10 mM Pseudomonas putida

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.1.1.B65 0.015
-
 NADP+ pH 8.5, 30°C Pseudomonas putida
1.1.1.B65 0.018
-
 NAD+ pH 8.5, 30°C Pseudomonas putida
1.1.1.B65 0.02
-
 (R)-mandelate pH 8.5, 30°C Pseudomonas putida

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.1.1.B65 K+ activates 44% at 1 mM and 69% at 10 mM Pseudomonas putida
1.1.1.B65 Mn2+ 21% activation at 1 mM, and 61% inhibition at 10 mM Pseudomonas putida
1.1.1.B65 Na+ activates 12% at 10 mM Pseudomonas putida

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.B65 (R)-mandelate + NAD+ Pseudomonas putida
-
 phenylglyoxylate + NADH + H+
-
 r
1.1.1.B65 (R)-mandelate + NAD+ Pseudomonas putida NUST506
-
 phenylglyoxylate + NADH + H+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.B65 Pseudomonas putida
-
 isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China)
-
1.1.1.B65 Pseudomonas putida NUST506
-
 isolated from soil around the schoolyard of Nanjing University of Science and Technology (Nanjing, China)
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.1.1.B65 native enzyme 11fold by ammonium sulfate fractionation and hydrophobic interaction chromatography Pseudomonas putida

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.1.1.B65 0.03
-
 crude enzyme extract, pH 8.5, 30°C Pseudomonas putida
1.1.1.B65 0.33
-
 purified native enzyme, pH 8.5, 30°C Pseudomonas putida

Storage Stability

EC Number Storage Stability Organism
1.1.1.B65 4°C, purified enzyme, 96 h, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5 Pseudomonas putida

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.B65 (R)-mandelate + NAD+
-
 Pseudomonas putida phenylglyoxylate + NADH + H+
-
 r
1.1.1.B65 (R)-mandelate + NAD+
-
 Pseudomonas putida NUST506 phenylglyoxylate + NADH + H+
-
 r
1.1.1.B65 (R)-mandelate + NADP+
-
 Pseudomonas putida phenylglyoxylate + NADPH + H+
-
 r
1.1.1.B65 (R)-mandelate + NADP+
-
 Pseudomonas putida NUST506 phenylglyoxylate + NADPH + H+
-
 r

Subunits

EC Number Subunits Comment Organism
1.1.1.B65 ? x * 61000, SDS-PAGE Pseudomonas putida

Synonyms

EC Number Synonyms Comment Organism
1.1.1.B65 RMDH
-
 Pseudomonas putida

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.1.1.B65 30
-
-
 Pseudomonas putida

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.1.1.B65 20 55 activity range, profile overview Pseudomonas putida

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.1.1.B65 30
-
 purified enzyme, pH 8.5, 31% activity remaining after 0.5 h, and 20% after 1 h Pseudomonas putida

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.1.1.B65 0.3
-
 NADP+ pH 8.5, 30°C Pseudomonas putida
1.1.1.B65 0.9
-
 NAD+ pH 8.5, 30°C Pseudomonas putida
1.1.1.B65 0.9
-
 (R)-mandelate pH 8.5, 30°C Pseudomonas putida

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.B65 8.5
-
-
 Pseudomonas putida

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.1.1.B65 5 10 activity range, profile overview Pseudomonas putida

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.1.1.B65 6 9 purified enzyme, the stability drops significantly at pH 8.5 and pH 9.0, the enzyme loses 85% and 94% of the activity when incubated for 3.5 h at 30°C, and it loses 11% of activity at pH 6.0 after 3.5 h at 30°C. After 96 h at 4°C, 78% of enzyme activity is retained at pH 6.0, and 30% at pH 8.5 Pseudomonas putida

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.B65 NAD+
-
 Pseudomonas putida
1.1.1.B65 NADH
-
 Pseudomonas putida
1.1.1.B65 NADP+
-
 Pseudomonas putida
1.1.1.B65 NADPH
-
 Pseudomonas putida