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Literature summary extracted from

  • Souza, F.; Meleiro, L.; Machado, C.; Zimbardi, A.; Maldonado, R.; Souza, T.; Masui, D.; Murakami, M.; Jorge, J.; Ward, R.; Furriel, R.
    Gene cloning, expression and biochemical characterization of a glucose- and xylose-stimulated beta-glucosidase from Humicola insolens RP86 (2014), J. Mol. Catal. B, 106, 1-10 .
No PubMed abstract available

Activating Compound

EC Number Activating Compound Comment Organism Structure
3.2.1.21 D-glucose 1.8fold stimulation at 50 mM Humicola insolens
3.2.1.21 D-xylose 1.9fold stimulation at 50 mM Humicola insolens

Cloned(Commentary)

EC Number Cloned (Comment) Organism
3.2.1.21 expression in Escherichia coli Humicola insolens

Inhibitors

EC Number Inhibitors Comment Organism Structure
3.2.1.21 L-arabinose 50 mM, 83% residual activity Humicola insolens
3.2.1.21 lactose 50 mM, 85% residual activity Humicola insolens
3.2.1.21 maltose 50 mM, 89% residual activity Humicola insolens

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
3.2.1.21 0.2
-
 4-nitrophenyl beta-D-glucopyranoside presence of D-glucose, pH 6.0, 50°C Humicola insolens
3.2.1.21 0.38
-
 cellobiose presence of D-glucose, pH 6.0, 50°C Humicola insolens

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
3.2.1.21 56100
-
 dynamic light scattering Humicola insolens

Organism

EC Number Organism UniProt Comment Textmining
3.2.1.21 Humicola insolens A0A076JRL8
-
-
3.2.1.21 Humicola insolens RP86 A0A076JRL8
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
3.2.1.21 1.4
-
 substrate 4-nitrophenyl beta-D-xylopyranoside, presence of D-glucose, pH 6.0, 50°C Humicola insolens
3.2.1.21 10.6
-
 substrate salicin, presence of D-glucose, pH 6.0, 50°C Humicola insolens
3.2.1.21 16.8
-
 substrate lactose, presence of D-glucose, pH 6.0, 50°C Humicola insolens
3.2.1.21 19.9
-
 substrate 4-nitrophenyl beta-D-galactopyranoside, presence of D-glucose, pH 6.0, 50°C Humicola insolens
3.2.1.21 36.4
-
 substrate 4-nitrophenyl beta-D-glucopyranoside, presence of D-glucose, pH 6.0, 50°C Humicola insolens
3.2.1.21 183.4
-
 substrate cellobiose, presence of D-glucose, pH 6.0, 50°C Humicola insolens

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
3.2.1.21 4-nitrophenyl beta-D-galactopyranoside + H2O
-
 Humicola insolens 4-nitrophenyl + D-galactose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Humicola insolens 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-glucopyranoside + H2O
-
 Humicola insolens RP86 4-nitrophenol + D-glucose
-
 ?
3.2.1.21 4-nitrophenyl beta-D-xylopyranoside + H2O
-
 Humicola insolens 4-nitrophenol + D-xylose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Humicola insolens 2 D-glucose
-
 ?
3.2.1.21 cellobiose + H2O
-
 Humicola insolens RP86 2 D-glucose
-
 ?
3.2.1.21 lactose + H2O
-
 Humicola insolens D-glucose + D-galactose
-
 ?
3.2.1.21 lactose + H2O
-
 Humicola insolens RP86 D-glucose + D-galactose
-
 ?
3.2.1.21 additional information broad substrate specificity, specific for substrates having beta-glycosidic linkages, with strong preference for glucose, followed by galactose, at the non-reducing end. No activity with sucrose, maltose and trehalose, carboxymethyl cellulose and avicel Humicola insolens ?
-
 ?
3.2.1.21 additional information broad substrate specificity, specific for substrates having beta-glycosidic linkages, with strong preference for glucose, followed by galactose, at the non-reducing end. No activity with sucrose, maltose and trehalose, carboxymethyl cellulose and avicel Humicola insolens RP86 ?
-
 ?
3.2.1.21 salicin + H2O
-
 Humicola insolens D-glucose + salicyl alcohol
-
 ?
3.2.1.21 salicin + H2O
-
 Humicola insolens RP86 D-glucose + salicyl alcohol
-
 ?

Subunits

EC Number Subunits Comment Organism
3.2.1.21 monomer 1 * 54100, calulated, 1 * 59000, SDS-PAGE of recombinant His-tagged protein Humicola insolens

Synonyms

EC Number Synonyms Comment Organism
3.2.1.21 Bglhi
-
 Humicola insolens

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
3.2.1.21 60
-
-
 Humicola insolens

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
3.2.1.21 50
-
 stable for 1 h Humicola insolens
3.2.1.21 55
-
 half-life 50 min Humicola insolens

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
3.2.1.21 33.9
-
 4-nitrophenyl beta-D-glucopyranoside presence of D-glucose, pH 6.0, 50°C Humicola insolens
3.2.1.21 172.1
-
 cellobiose presence of D-glucose, pH 6.0, 50°C Humicola insolens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
3.2.1.21 5 7
-
 Humicola insolens

pH Range

EC Number pH Minimum pH Maximum Comment Organism
3.2.1.21 3
-
 70% residual activity Humicola insolens

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
3.2.1.21 4.5 8
-
 Humicola insolens

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
3.2.1.21 169
-
 4-nitrophenyl beta-D-glucopyranoside presence of D-glucose, pH 6.0, 50°C Humicola insolens
3.2.1.21 453
-
 cellobiose presence of D-glucose, pH 6.0, 50°C Humicola insolens