EC Number | Cloned (Comment) | Organism |
---|---|---|
1.8.1.4 | gene dld, located on chromosome 7, recombinant expression of His-tagged wild-type and mutant enzymes in Escherichia coli strain XL-1 Blue | Homo sapiens |
EC Number | Protein Variants | Comment | Organism |
---|---|---|---|
1.8.1.4 | P156A | site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme | Homo sapiens |
1.8.1.4 | P303A | site-directed mutagenesis, the mutant shows reduced catalytic efficiency compared to the wild-type enzyme | Homo sapiens |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.4 | 0.19 | - |
NAD+ | wild-type enzyme, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 0.24 | - |
NAD+ | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 0.42 | - |
NAD+ | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 0.55 | - |
dihydrolipoamide | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 0.63 | - |
dihydrolipoamide | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 0.64 | - |
dihydrolipoamide | wild-type enzyme, pH 8.0, 37°C | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.8.1.4 | mitochondrion | E3 is a mitochondrial protein that is synthesized in precursor form in the cytoplasm. The precursor E3 for humans has a leader sequence composed of 35-amino acids at its amino-terminus, which is cleaved off during its import into the mitochondria matrix | Homo sapiens | 5739 | - |
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.4 | dihydrolipoamide + NAD+ | Homo sapiens | - |
lipoamide + NADH + H+ | - |
r |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.8.1.4 | Homo sapiens | P09622 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.8.1.4 | recombinant His-tagged wild-type and mutant enzymes from Escherichia coli strain XL-1 Blue by nickel affinity chromatography and dialysis | Homo sapiens |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.8.1.4 | dihydrolipoamide + NAD+ | - |
Homo sapiens | lipoamide + NADH + H+ | - |
r |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.8.1.4 | homodimer | 2 * 50216, sequence calculation | Homo sapiens |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.8.1.4 | dihydrolipoamide dehydrogenase | - |
Homo sapiens |
1.8.1.4 | dihydrolipoamide:NAD+ oxidoreductase | - |
Homo sapiens |
1.8.1.4 | DLD | - |
Homo sapiens |
1.8.1.4 | DLDH | - |
Homo sapiens |
1.8.1.4 | E3 | - |
Homo sapiens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.8.1.4 | 37 | - |
assay at | Homo sapiens |
EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.4 | 629 | - |
NAD+ | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 629 | - |
dihydrolipoamide | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 817 | - |
NAD+ | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 817 | - |
dihydrolipoamide | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 899 | - |
NAD+ | wild-type enzyme, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 899 | - |
dihydrolipoamide | wild-type enzyme, pH 8.0, 37°C | Homo sapiens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.8.1.4 | 8 | - |
assay at | Homo sapiens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.8.1.4 | FAD | one FAD as a prosthetic group at each subunit, binding structure analysis | Homo sapiens | |
1.8.1.4 | NAD+ | binding structure analysis | Homo sapiens | |
1.8.1.4 | NADH | - |
Homo sapiens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.8.1.4 | evolution | residues Pro156 and Pro303 are highly conserved | Homo sapiens |
1.8.1.4 | additional information | location of residues Pro156 and Pro303 in human E3 enzyme, structure comparisons with E3 enzymes from other species | Homo sapiens |
EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.8.1.4 | 1143.6 | - |
dihydrolipoamide | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 1296.8 | - |
dihydrolipoamide | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 1404.7 | - |
dihydrolipoamide | wild-type enzyme, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 1497.6 | - |
NAD+ | enzyme mutant P303A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 3404.2 | - |
NAD+ | enzyme mutant P156A, pH 8.0, 37°C | Homo sapiens | |
1.8.1.4 | 4731.6 | - |
NAD+ | wild-type enzyme, pH 8.0, 37°C | Homo sapiens |