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Literature summary extracted from

  • Sakurai, N.; Kataoka, K.; Sugaya, N.; Shimodaira, T.; Iwamoto, M.; Shoda, M.; Horiuchi, H.; Kiyono, M.; Ohta, Y.; Triwiyono, B.; Seo, D.; Sakurai, T.
    Heterologous expression of Halomonas halodenitrificans nitric oxide reductase and its N-terminally truncated NorC subunit in Escherichia coli (2017), J. Inorg. Biochem., 169, 61-67 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.7.2.5 gene norC, recombinant expression of wild-type and mutant NorC subunits in Escherichia coli strain BL21(DE3) Halomonas halodenitrificans

Protein Variants

EC Number Protein Variants Comment Organism
1.7.2.5 additional information construction of a truncated soluble domain of NorC, NorC* (DELTAMet1-Val37), by deletion of the 84 5'-terminal nucleotides of gene norC. The mutant NorC exhibits spectra typical of a low-spin heme c. In addition, NorC* functions as the acceptor of an electron from a cytochrome c isolated from the periplasm of Halomonas halodenitrificans and small reducing reagents. The redox potential of NorC* shifted ca. 40mV in the negative direction from that of wild-type NorC. Recombinant NOR exhibits the same spectroscopic properties and reactivity to NO and O2 as wil-type NOR, although its enzymatic activity toward NO is considerably decreased Halomonas halodenitrificans

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.7.2.5 inner membrane NorB is themembrane-spanning subunit Halomonas halodenitrificans
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Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.7.2.5 Fe2+ subunit NorB contains a low-spin heme b center, a high-spin heme b3 center, and a non-heme FeB center Halomonas halodenitrificans

Organism

EC Number Organism UniProt Comment Textmining
1.7.2.5 Halomonas halodenitrificans O50651 small subunit encoded by gene norC; formerly Paracoccus halodenitrificans
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Purification (Commentary)

EC Number Purification (Comment) Organism
1.7.2.5 recombinant wild-type and mutant NorC subunits from Escherichia coli strain BL21(DE3) by anion exchange chromatography, gel filtration, and again ion exchange chromatography Halomonas halodenitrificans

Subunits

EC Number Subunits Comment Organism
1.7.2.5 heterodimer membrane-bound heterodimer complex of NorC, which contains a low-spin heme c center, and NorB, which contains a low-spin heme b center, a high-spin heme b3 center, and a non-heme FeB center. NorB is themembrane-spanning subunit, it contains 12alpha-helices Halomonas halodenitrificans

Synonyms

EC Number Synonyms Comment Organism
1.7.2.5 nitric oxide reductase
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 Halomonas halodenitrificans

Cofactor

EC Number Cofactor Comment Organism Structure
1.7.2.5 cytochrome c
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 Halomonas halodenitrificans
1.7.2.5 heme subunit NorB contains a low-spin heme b center, a high-spin heme b3 center, and a non-heme FeB center Halomonas halodenitrificans

General Information

EC Number General Information Comment Organism
1.7.2.5 malfunction recombinant truncated NOR exhibits the same spectroscopic properties and reactivity to NO and O2 as wil-type NOR, although its enzymatic activity toward NO is considerably decreased Halomonas halodenitrificans
1.7.2.5 additional information Halomonas halodenitrificans nitric oxide reductase (NOR) is the membrane-bound heterodimer complex of NorC, which contains a low-spin heme c center, and NorB, which contains a low-spin heme b center, a high-spin heme b3 center, and a non-heme FeB center Halomonas halodenitrificans
1.7.2.5 physiological function NO reductase (NOR) protects denitrifying bacteria from NO, which is highly toxic, by converting it to N2O Halomonas halodenitrificans