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Literature summary extracted from

  • Shao, M.; Zhang, X.; Rao, Z.; Xu, M.; Yang, T.; Li, H.; Xu, Z.; Yang, S.
    A mutant form of 3-ketosteroid-Delta(1)-dehydrogenase gives altered androst-1,4-diene-3, 17-dione/androst-4-ene-3,17-dione molar ratios in steroid biotransformations by Mycobacterium neoaurum ST-095 (2016), J. Ind. Microbiol. Biotechnol., 43, 691-701 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.99.4
-
 Mycolicibacterium neoaurum

Protein Variants

EC Number Protein Variants Comment Organism
1.3.99.4 additional information enzyme from strain ST-095 only differs in residue V366 from strain JC-12 (S366). Mutation results in almost 3fold higher catalytic efficiency in strain JC-12 Mycolicibacterium neoaurum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.99.4 0.011
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum
1.3.99.4 0.034
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum

Organism

EC Number Organism UniProt Comment Textmining
1.3.99.4 Mycolicibacterium neoaurum
-
-
-
1.3.99.4 Mycolicibacterium neoaurum JC-12
-
-
-
1.3.99.4 Mycolicibacterium neoaurum ST-095
-
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.99.4 1.46
-
 pH 7.0, 30°C Mycolicibacterium neoaurum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.99.4 4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Mycolicibacterium neoaurum 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Mycolicibacterium neoaurum JC-12 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?
1.3.99.4 4-androstene-3,17-dione + 2,6-dichlorophenol-indophenol
-
 Mycolicibacterium neoaurum ST-095 1,4-androstadiene-3,17-dione + reduced 2,6-dichlorophenol-indophenol
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.99.4 ? x * 61000, SDS-PAGE Mycolicibacterium neoaurum

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.99.4 0.04
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum
1.3.99.4 0.096
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.3.99.4 1.17
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum
1.3.99.4 8.83
-
 4-androstene-3,17-dione pH 7.0, 30°C Mycolicibacterium neoaurum