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Literature summary extracted from

  • Weyda, I.; Luebeck, M.; Ahring, B.K.; Luebeck, P.S.
    Point mutation of the xylose reductase (XR) gene reduces xylitol accumulation and increases citric acid production in Aspergillus carbonarius (2014), J. Ind. Microbiol. Biotechnol., 41, 733-739 .
    View publication on PubMedView publication on EuropePMC

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.1.1.9 NADH product inhibition Aspergillus carbonarius

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.9 xylitol + NAD+ Aspergillus carbonarius
-
D-xylulose + NADH + H+
-
?
1.1.1.9 xylitol + NAD+ Aspergillus carbonarius ITEM 5010
-
D-xylulose + NADH + H+
-
?

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.9 Aspergillus carbonarius
-
-
-
1.1.1.9 Aspergillus carbonarius ITEM 5010
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.9 xylitol + NAD+
-
Aspergillus carbonarius D-xylulose + NADH + H+
-
?
1.1.1.9 xylitol + NAD+
-
Aspergillus carbonarius ITEM 5010 D-xylulose + NADH + H+
-
?

Synonyms

EC Number Synonyms Comment Organism
1.1.1.9 XDH
-
Aspergillus carbonarius

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.9 NAD+
-
Aspergillus carbonarius

General Information

EC Number General Information Comment Organism
1.1.1.9 metabolism in order to overcome xylitol accumulation in Aspergillus carbonarius, a K274R point mutation is introduced into the xylose reductase with the aim of changing the specificity toward NADH. Fermentation with the mutant strain (grown on D-xylose as the sole carbon source) shows a 2.8fold reduction in xylitol accumulation and 4.5fold increase in citric acid production compared to the wild-type strain. The fact that the mutant strain shows decreased xylitol levels is assumed to be associated with the capability of the mutated xylose reductase to use NADH generated by the xylitol dehydrogenase XDH, thus preventing the inhibition of XDH by the high levels of NADH and ensuring the flux of D-xylose through the pathway Aspergillus carbonarius