EC Number | Activating Compound | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | glycerol | 20% (v/v), 15% increase inactivity | Trichoderma harzianum | |
1.10.3.2 | Tween-40 | 0.5% (v/v), 10% increase in activity | Trichoderma harzianum |
EC Number | Application | Comment | Organism |
---|---|---|---|
1.10.3.2 | degradation | laccase can be efficiently used to decolorize synthetic dye and help in waste water treatment | Trichoderma harzianum |
1.10.3.2 | environmental protection | laccase can be efficiently used to decolorize synthetic dye and is a suitable candidate for the treatment of wastewater from industrial effluents. The wide pH- and thermostability attributes of immobilized laccase make them suitable for environmental applications | Trichoderma harzianum |
EC Number | General Stability | Organism |
---|---|---|
1.10.3.2 | Ca-alginate, Cu-alginate, Ca-alginate-chitosan and solĀgel immobilized laccase show an operational stability of 36%, 51%, 66% and 82%, respectively after 6 cycles | Trichoderma harzianum |
1.10.3.2 | soluble enzyme loses 45% of its activity after 8 days at 4Ā°C. Sol-gel matrix immobilized laccase is the most stable with a mere loss of 2% among the Ca-alginate-chitosan, Cu-alginate and Ca-alginate beads with a loss of 10%, 17% and 25%, respectively | Trichoderma harzianum |
EC Number | Inhibitors | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | 2-mercaptoethanol | 5 mM, 15% loss of activity | Trichoderma harzianum | |
1.10.3.2 | acetone | 20% (v/v), 42% loss of activity | Trichoderma harzianum | |
1.10.3.2 | Al3+ | 5 mM, 60% loss of activity | Trichoderma harzianum | |
1.10.3.2 | Cd2+ | 5 mM, 78% loss of activity | Trichoderma harzianum | |
1.10.3.2 | Co2+ | 5 mM, 67% loss of activity | Trichoderma harzianum | |
1.10.3.2 | dithiothreitol | 5 mM, 65% loss of activity | Trichoderma harzianum | |
1.10.3.2 | EDTA | 5 mM, 70% loss of activity | Trichoderma harzianum | |
1.10.3.2 | ethanol | 20% (v/v), 15% loss of activity | Trichoderma harzianum | |
1.10.3.2 | Fe2+ | 5 mM, 60% loss of activity | Trichoderma harzianum | |
1.10.3.2 | Hg2+ | 5 mM, 90% loss of activity | Trichoderma harzianum | |
1.10.3.2 | Isopropanol | 20% (v/v), 30% loss of activity | Trichoderma harzianum | |
1.10.3.2 | L-cysteine | 5 mM, 20% loss of activity | Trichoderma harzianum | |
1.10.3.2 | methanol | 20% (v/v), 20% loss of activity | Trichoderma harzianum | |
1.10.3.2 | additional information | soluble laccase shows more than 80% relative activity in organic solvents like ethanol, methanol, toluene and acetonitrile | Trichoderma harzianum | |
1.10.3.2 | Ni2+ | 5 mM, 35% loss of activity | Trichoderma harzianum | |
1.10.3.2 | SDS | 0.5%, 60% loss of activity | Trichoderma harzianum | |
1.10.3.2 | Toluene | 20% (v/v), 18% loss of activity | Trichoderma harzianum |
EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|---|
1.10.3.2 | 0.5 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 6, 25Ā°C, soluble enzyme | Trichoderma harzianum | |
1.10.3.2 | 2 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 6, 25Ā°C,laccase immobilized in solĀgel matrix | Trichoderma harzianum |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|---|
1.10.3.2 | extracellular | - |
Trichoderma harzianum | - |
- |
EC Number | Metals/Ions | Comment | Organism | Structure |
---|---|---|---|---|
1.10.3.2 | Ca2+ | 5 mM, 18% increase in activity | Trichoderma harzianum | |
1.10.3.2 | Cu2+ | 5 mM, 85% increase in activity | Trichoderma harzianum | |
1.10.3.2 | Mg2+ | 5 mM, 15% increase in activity | Trichoderma harzianum | |
1.10.3.2 | Mn2+ | 5 mM, 8% increase in activity | Trichoderma harzianum |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.10.3.2 | Trichoderma harzianum | - |
- |
- |
1.10.3.2 | Trichoderma harzianum HZN10 | - |
- |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.10.3.2 | - |
Trichoderma harzianum |
EC Number | Specific Activity Minimum [Āµmol/min/mg] | Specific Activity Maximum [Āµmol/min/mg] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 164 | - |
pH 6, 25Ā°C | Trichoderma harzianum |
EC Number | Storage Stability | Organism |
---|---|---|
1.10.3.2 | 4Ā°C, 8 days, soluble enzyme loses 45% of its activity. SolĀgel matrix immobilized laccase is the most stable with a mere loss of 2% among the Ca-alginate-chitosan, Cu-alginate and Ca-alginate beads with a loss of 10%, 17% and 25%, respectively | Trichoderma harzianum |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.10.3.2 | 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Trichoderma harzianum | ? | - |
? | |
1.10.3.2 | 2,6-dimethoxyphenol + O2 | 96% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
1.10.3.2 | catechol + O2 | 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
1.10.3.2 | catechol + O2 | 44% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum HZN10 | ? | - |
? | |
1.10.3.2 | ferulic acid + O2 | 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
1.10.3.2 | ferulic acid + O2 | 24% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum HZN10 | ? | - |
? | |
1.10.3.2 | gallic acid + O2 | 54% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
1.10.3.2 | gallic acid + O2 | 54% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum HZN10 | ? | - |
? | |
1.10.3.2 | guaiacol + O2 | 83% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
1.10.3.2 | additional information | free and immobilized laccase is employed for decolorization of three different synthetic dyes (malachite green, methylene blue and congo red). Approximately 100% of malachite green, 90% of methylene blue and 60% of congo red dyes at initial concentration of 200 mg/l are decolorized within 16, 18 and 20 h, respectively by laccase immobilized in sol-gel matrix in the presence of 1-hydroxybenzotriazole mediator | Trichoderma harzianum | ? | - |
? | |
1.10.3.2 | additional information | free and immobilized laccase is employed for decolorization of three different synthetic dyes (malachite green, methylene blue and congo red). Approximately 100% of malachite green, 90% of methylene blue and 60% of congo red dyes at initial concentration of 200 mg/l are decolorized within 16, 18 and 20 h, respectively by laccase immobilized in sol-gel matrix in the presence of 1-hydroxybenzotriazole mediator | Trichoderma harzianum HZN10 | ? | - |
? | |
1.10.3.2 | syringaldazine + O2 | 148% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum | ? | - |
? | |
1.10.3.2 | syringaldazine + O2 | 148% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Trichoderma harzianum HZN10 | ? | - |
? |
EC Number | Subunits | Comment | Organism |
---|---|---|---|
1.10.3.2 | ? | x * 56000, SDS-PAGE | Trichoderma harzianum |
EC Number | Temperature Optimum [Ā°C] | Temperature Optimum Maximum [Ā°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 50 | - |
soluble and immobilized laccases | Trichoderma harzianum |
EC Number | Temperature Minimum [Ā°C] | Temperature Maximum [Ā°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 50 | 70 | all the forms of immobilized laccase show more than 90% relative activity at higher temperature of 55Ā°C and 60Ā°C. Sol-gel immobilized laccase shows 97% (65Ā°C) and 94% (70Ā°C) relative activity at higher temperatures. Soluble enzyme loses maximum activity above 55Ā°C | Trichoderma harzianum |
EC Number | Temperature Stability Minimum [Ā°C] | Temperature Stability Maximum [Ā°C] | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 50 | - |
4 h, soluble and immobilized laccases retain more than 90% activity at 50Ā°C. Copper alginate shows better stability than calcium alginate immobilized laccase | Trichoderma harzianum |
1.10.3.2 | 55 | - |
4 h, calcium alginate-chitosan immobilized laccase retains 91% activity | Trichoderma harzianum |
1.10.3.2 | 65 | - |
4 h, sol-gel immobilized laccase retains 90% activity | Trichoderma harzianum |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 4 | 7 | laccase immobilized in sol-gel matrix | Trichoderma harzianum |
1.10.3.2 | 5 | 6 | copper alginate and calcium alginate-chitosan immobilized laccase | Trichoderma harzianum |
1.10.3.2 | 6 | - |
soluble enzyme and calcium alginate immobilized enzyme | Trichoderma harzianum |
EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|---|
1.10.3.2 | 4 | - |
4 h, solĀgel immobilized laccase retains 98% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀgel matrix > calcium alginateĀchitosan > copper alginate > calcium alginate | Trichoderma harzianum |
1.10.3.2 | 5 | 6 | 4 h, solĀgel immobilized laccase retains 100% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀgel matrix > calcium alginateĀchitosan > copper alginate > calcium alginate | Trichoderma harzianum |
1.10.3.2 | 7 | - |
4 h, solĀgel immobilized laccase retains 96% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀgel matrix > calcium alginateĀchitosan > copper alginate > calcium alginate | Trichoderma harzianum |
1.10.3.2 | 8 | - |
4 h, solĀgel immobilized laccase retains 92% of the activity. In comparison to soluble laccase better stability is observed in the order, solĀgel matrix > calcium alginateĀchitosan > copper alginate > calcium alginate | Trichoderma harzianum |