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Literature summary extracted from
- Purification and characterization of an extracellular beta-glucosidase from the thermophilic fungus Sporotrichum thermophile and its influence on cellulase activity (1993), J. Gen. Microbiol., 139, 2825-2832 .
No PubMed abstract available
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 3.2.1.21 | D-fucose | 10 mM, 68% inhibition, competitive | Thermothelomyces thermophilus |  |
| 3.2.1.21 | D-gluconolactone | 10 mM, 80% inhibition, competitive | Thermothelomyces thermophilus | |
| 3.2.1.21 | D-glucose | 10 mM, 80% inhibition, competitive | Thermothelomyces thermophilus | |
| 3.2.1.21 | maltose | 10 mM, 65% inhibition, competitive | Thermothelomyces thermophilus | |
| 3.2.1.21 | Nojirimycin | 10 mM, 80% inhibition, competitive | Thermothelomyces thermophilus | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.29 | - |
4-nitrophenyl beta-D-glucose | pH 5.4, 50°C | Thermothelomyces thermophilus | |
| 3.2.1.21 | 0.35 | - |
gentiobiose | pH 5.4, 50°C | Thermothelomyces thermophilus | |
| 3.2.1.21 | 0.83 | - |
cellobiose | pH 5.4, 50°C | Thermothelomyces thermophilus | |
Localization
| EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|---|
| 3.2.1.21 | extracellular | - |
Thermothelomyces thermophilus | - |
- |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 3.2.1.21 | Thermothelomyces thermophilus | - |
- |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 3.2.1.21 | after growth on cellulose | Thermothelomyces thermophilus |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 89 | - |
substrate 4-nitrophenyl beta-D-glucose, pH 5.4, 50°C | Thermothelomyces thermophilus |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 3.2.1.21 | 4-nitrophenyl beta-D-glucose + H2O | - |
Thermothelomyces thermophilus | 4-nitrophenyl + D-glucose | - |
? | |
| 3.2.1.21 | cellobiose + H2O | - |
Thermothelomyces thermophilus | 2 D-glucose | - |
? | |
| 3.2.1.21 | cellohexaose + 5 H2O | - |
Thermothelomyces thermophilus | 6 D-glucose | - |
? | |
| 3.2.1.21 | cellopentaose + 4 H2O | - |
Thermothelomyces thermophilus | 5 D-glucose | - |
? | |
| 3.2.1.21 | cellotetraose + 3 H2O | - |
Thermothelomyces thermophilus | 4 D-glucose | - |
? | |
| 3.2.1.21 | cellotriose + 2 H2O | - |
Thermothelomyces thermophilus | 3 D-glucose | - |
? | |
| 3.2.1.21 | esculin + H2O | - |
Thermothelomyces thermophilus | esculetin + beta-D-glucose | - |
? | |
| 3.2.1.21 | gentiobiose + H2O | - |
Thermothelomyces thermophilus | 2 D-glucose | - |
? | |
| 3.2.1.21 | laminaribiose + H2O | - |
Thermothelomyces thermophilus | 2 D-glucose | - |
? | |
| 3.2.1.21 | additional information | above 1 mM substrate concentration, enzyme catalyses a parallel transglycosylation reaction. No substrate: beta-1,4-glucan | Thermothelomyces thermophilus | ? | - |
? | |
| 3.2.1.21 | salicin + H2O | - |
Thermothelomyces thermophilus | D-glucose + salicyl alcohol | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 65 | - |
- |
Thermothelomyces thermophilus |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 50 | - |
pH 5.4, stable for 6 h | Thermothelomyces thermophilus |
| 3.2.1.21 | 60 | - |
2 h, 25% loss of activity | Thermothelomyces thermophilus |
| 3.2.1.21 | 70 | - |
complete loss of activity within 1 h | Thermothelomyces thermophilus |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 5.4 | - |
- |
Thermothelomyces thermophilus |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 3.2.1.21 | 4 | 6.5 | 50°C, stable for at least 6 h | Thermothelomyces thermophilus |
Ki Value [mM]
| EC Number | Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 3.2.1.21 | 0.00026 | - |
Nojirimycin | pH 5.4, 50°C | Thermothelomyces thermophilus | |
| 3.2.1.21 | 0.008 | - |
D-gluconolactone | pH 5.4, 50°C | Thermothelomyces thermophilus | |
| 3.2.1.21 | 0.5 | - |
D-glucose | pH 5.4, 50°C | Thermothelomyces thermophilus | |
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