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Literature summary extracted from

  • El Hakim, A.; Salama, W.; Hamed, M.; Ali, A.; Ibrahim, N.
    Heterodimeric lL-amino acid oxidase enzymes from Egyptian Cerastes cerastes venom Purification, biochemical characterization and partial amino acid sequencing (2015), J. Gen. Eng. Biotechnol., 13, 165-176 .
No PubMed abstract available

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.4.3.2 1,10-phenanthroline complete inhibition of isoform LAAOII and about 30% inhibition of isoform LAAOI at 5 mM Cerastes cerastes
1.4.3.2 2-mercaptoethanol complete inhibition of isoform LAAOII and about 50% inhibition of isoform LAAOI at 5 mM Cerastes cerastes
1.4.3.2 Al3+ strong inhibition of both isoforms at 10 mM Cerastes cerastes
1.4.3.2 Co2+ strong inhibition of both isoforms at 10 mM Cerastes cerastes
1.4.3.2 Cu2+ strong inhibition of both isoforms at 10 mM Cerastes cerastes
1.4.3.2 EDTA about 40% inhibition of isoform LAAOII and about 10% inhibition of isoform LAAOI at 5 mM Cerastes cerastes
1.4.3.2 iodoacetic acid about 45% inhibition of isoform LAAOII and no inhibition of isoform LAAOI at 5 mM Cerastes cerastes
1.4.3.2 L-cysteine complete inhibition of both isoforms at 5 mM Cerastes cerastes
1.4.3.2 Ni2+ strong inhibition of both isoforms at 10 mM Cerastes cerastes
1.4.3.2 phenylmethylsulfonyl fluoride about 20% inhibition of isoform LAAOII and about 90% inhibition of isoform LAAOI at 5 mM Cerastes cerastes
1.4.3.2 reduced glutathione complete inhibition of both isoforms at 5 mM Cerastes cerastes
1.4.3.2 Urea about 50% inhibition of isoform LAAOII and about 20% inhibition of isoform LAAOI at 5 mM Cerastes cerastes
1.4.3.2 Zn2+ strong inhibition of both isoforms at 10 mM Cerastes cerastes

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.2 0.67
-
 L-Leu isoform LAAOI, at pH 7.8 and 50°C Cerastes cerastes
1.4.3.2 0.82
-
 L-Leu isoform LAAOII, at pH 7.0 and 60°C Cerastes cerastes

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.4.3.2 Mn2+ 10 mM Mn2+ increases the activity of isoform LAAOII more than 2fold Cerastes cerastes
1.4.3.2 additional information Na+, K+, Ca2+, Mg2+ and Ba2+ ions show a non-significant increase in the enzymatic activity Cerastes cerastes

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.4.3.2 115000
-
 gel filtration Cerastes cerastes

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.2 Cerastes cerastes X2JCV5
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.4.3.2 DEAE Sepharose column chromatography, and Superdex 200 gel filtration Cerastes cerastes

Source Tissue

EC Number Source Tissue Comment Organism Textmining
1.4.3.2 venom
-
 Cerastes cerastes
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.2 L-Arg + H2O + O2 best substrate Cerastes cerastes 2-oxo-5-guanidinovaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Ile + H2O + O2
-
 Cerastes cerastes 3-methyl-2-oxo-pentanoic acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Leu + H2O + O2
-
 Cerastes cerastes 2-oxo-4-methylvaleric acid + NH3 + H2O2
-
 ?
1.4.3.2 L-Met + H2O + O2
-
 Cerastes cerastes 4-methylsulfanyl-2-oxobutanoate + NH3 + H2O2
-
 ?
1.4.3.2 L-Phe + H2O + O2
-
 Cerastes cerastes phenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Trp + H2O + O2
-
 Cerastes cerastes indole-3-pyruvate + NH3 + H2O2
-
 ?
1.4.3.2 L-Tyr + H2O + O2
-
 Cerastes cerastes 4-hydroxyphenylpyruvate + NH3 + H2O2
-
 ?
1.4.3.2 additional information the enzyme isoforms show low affinity for L-Val, L-Ala, L-Asn, L-Gln and no specificity toward L-Pro, L-Ser, L-Thr, L-Cys, L-Gly and L-Asp Cerastes cerastes ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.4.3.2 heterodimer 1 * 60000 + 1 * 53000, isoform LAAOII, SDS-PAGE Cerastes cerastes
1.4.3.2 heterodimer 1 * 60000 + 1 * 56000, isoform LAAOI, SDS-PAGE Cerastes cerastes

Synonyms

EC Number Synonyms Comment Organism
1.4.3.2 LAAOI isoform Cerastes cerastes
1.4.3.2 LAAOII isoform Cerastes cerastes

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.4.3.2 50
-
 isoform LAAOI Cerastes cerastes
1.4.3.2 60
-
 isoform LAAOI Cerastes cerastes

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.4.3.2 50 70 the enzyme isoforms are thermally stable up to 70°C for 1 h since no loss of enzymatic activities are recorded up to 40°C for isoform LAAOI and 50°C for LAAOII, whereas 50% loss of the enzymatic activities are recorded at 60°C for isoform LAAOI and 65°C for isoform LAAOII, respectively. On the other hand, a complete loss of activities is detected above 70°C for both isoforms Cerastes cerastes

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.4.3.2 7
-
 isoform LAAOI Cerastes cerastes
1.4.3.2 7.8
-
 isoform LAAOI Cerastes cerastes

pH Stability

EC Number pH Stability pH Stability Maximum Comment Organism
1.4.3.2 4 9 both enzyme isoform maintain their full activity at a wide range of pH from 4.0 to 9.0 for 1 h Cerastes cerastes