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Literature summary extracted from

  • Xiao, X.; Si, M.; Yang, Z.; Zhang, Y.; Guan, J.; Chaudhry, M.T.; Wang, Y.; Shen, X.
    Molecular characterization of a eukaryotic-like phenol hydroxylase from Corynebacterium glutamicum (2015), J. Gen. Appl. Microbiol., 61, 99-107 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.14.13.7 expressed in Escherichia coli BL21(DE3) cells Corynebacterium glutamicum

Protein Variants

EC Number Protein Variants Comment Organism
1.14.13.7 C349S the mutant shows 30% activity compared to the wild type enzyme Corynebacterium glutamicum
1.14.13.7 C476S the mutant shows 45% activity compared to the wild type enzyme Corynebacterium glutamicum
1.14.13.7 D75N the mutant shows 288% activity compared to the wild type enzyme Corynebacterium glutamicum
1.14.13.7 P261S the mutant shows 15% activity compared to the wild type enzyme Corynebacterium glutamicum
1.14.13.7 R262S the mutant shows 427% activity compared to the wild type enzyme Corynebacterium glutamicum
1.14.13.7 R269L the mutant shows 232% activity compared to the wild type enzyme Corynebacterium glutamicum

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.14.13.7 Co2+ 63% residual activity at 1 mM Corynebacterium glutamicum
1.14.13.7 Cu2+ 37% residual activity at 0.02 mM Corynebacterium glutamicum
1.14.13.7 Fe2+ complete inhibition at 1 mM Corynebacterium glutamicum
1.14.13.7 Fe3+ 3% residual activity at 1 mM Corynebacterium glutamicum
1.14.13.7 Ni2+ 4% residual activity at 1 mM Corynebacterium glutamicum
1.14.13.7 Zn2+ 13% residual activity at 1 mM Corynebacterium glutamicum

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.14.13.7 0.256
-
 phenol at pH 8.0 and 45°C Corynebacterium glutamicum
1.14.13.7 0.284
-
 NADPH at pH 8.0 and 45°C Corynebacterium glutamicum

Metals/Ions

EC Number Metals/Ions Comment Organism Structure
1.14.13.7 Mg2+ 119% activity at 1 mM Corynebacterium glutamicum
1.14.13.7 Mn2+ 121% activity at 1 mM Corynebacterium glutamicum

Molecular Weight [Da]

EC Number Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
1.14.13.7 140000
-
 gel filtration Corynebacterium glutamicum

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.14.13.7 phenol + NADPH + H+ + O2 Corynebacterium glutamicum
-
 catechol + NADP+ + H2O
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.14.13.7 Corynebacterium glutamicum
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.14.13.7 His-bind Ni-NTA resin column chromatography Corynebacterium glutamicum

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.14.13.7 1,3-dihydroxybenzene + NADPH + H+ + O2 50.12% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
1.14.13.7 2-hydroxybenzoic acid + NADPH + H+ + O2 15.97% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
1.14.13.7 2-naphthol + NADPH + H+ + O2 9.75% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
1.14.13.7 3-hydroxybenzoic acid + NADPH + H+ + O2 18.53% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
1.14.13.7 4-chlorophenol + NADPH + H+ + O2 28.6% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
1.14.13.7 4-hydroxybenzoic acid + NADPH + H+ + O2 14.88% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
1.14.13.7 hydroquinone + NADPH + H+ + O2 48.25% activity compared to phenol Corynebacterium glutamicum ? + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADH + H+ + O2 activity is reduced by 84% when NADPH is replaced by NADH Corynebacterium glutamicum catechol + NAD+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2
-
 Corynebacterium glutamicum catechol + NADP+ + H2O
-
 ?
1.14.13.7 phenol + NADPH + H+ + O2 100% activity Corynebacterium glutamicum catechol + NADP+ + H2O
-
 ?

Subunits

EC Number Subunits Comment Organism
1.14.13.7 homodimer 2 * 70000, SDS-PAGE Corynebacterium glutamicum

Synonyms

EC Number Synonyms Comment Organism
1.14.13.7 NCgl2588
-
 Corynebacterium glutamicum

Temperature Optimum [°C]

EC Number Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
1.14.13.7 45
-
-
 Corynebacterium glutamicum

Temperature Range [°C]

EC Number Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
1.14.13.7 40 50 more than 50% activity between 40 and 50°C Corynebacterium glutamicum

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.14.13.7 8
-
-
 Corynebacterium glutamicum

pH Range

EC Number pH Minimum pH Maximum Comment Organism
1.14.13.7 7 9 more than 80% activity between pH 7.0 and 9.0 Corynebacterium glutamicum

Cofactor

EC Number Cofactor Comment Organism Structure
1.14.13.7 FAD
-
 Corynebacterium glutamicum
1.14.13.7 NADPH the enzyme has a preference for NADPH as a cofactor Corynebacterium glutamicum

pI Value

EC Number Organism Comment pI Value Maximum pI Value
1.14.13.7 Corynebacterium glutamicum calculated from amino acid sequence
-
 4.9