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Literature summary extracted from

  • Sonawane, P.; Vishwakarma, R.K.; Singh, S.; Gaikwad, S.; Khan, B.M.
    Steady state fluorescence studies of wild type recombinant cinnamoyl CoA reductase (Ll-CCRH1) and its active site mutants (2014), J. Fluoresc., 24, 665-673 .
    View publication on PubMed

Protein Variants

EC Number Protein Variants Comment Organism
1.2.1.44 D77G site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
1.2.1.44 F30V site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
1.2.1.44 H215L site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
1.2.1.44 I31N site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
1.2.1.44 K174M site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
1.2.1.44 R51G site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
1.2.1.44 S136A site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
1.2.1.44 S212G site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
1.2.1.44 V200E site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala
1.2.1.44 Y170H site-directed mutagenesis, structure comparison to the wild-type enzyme Leucaena leucocephala

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.2.1.44 feruloyl-CoA + NADPH + H+ Leucaena leucocephala
-
 coniferaldehyde + CoA + NADP+
-
 r
1.2.1.44 sinapoyl-CoA + NADPH + H+ Leucaena leucocephala
-
 sinapaldehyde + CoA + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.2.1.44 Leucaena leucocephala Q08GL0
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.2.1.44 feruloyl-CoA + NADPH + H+
-
 Leucaena leucocephala coniferaldehyde + CoA + NADP+
-
 r
1.2.1.44 feruloyl-CoA + NADPH + H+ best substrate Leucaena leucocephala coniferaldehyde + CoA + NADP+
-
 r
1.2.1.44 sinapoyl-CoA + NADPH + H+
-
 Leucaena leucocephala sinapaldehyde + CoA + NADP+
-
 r

Synonyms

EC Number Synonyms Comment Organism
1.2.1.44 CCR
-
 Leucaena leucocephala
1.2.1.44 cinnamoyl CoA reductase
-
 Leucaena leucocephala
1.2.1.44 cinnamoyl CoA reductase 1
-
 Leucaena leucocephala
1.2.1.44 Ll-CCRH1
-
 Leucaena leucocephala

Cofactor

EC Number Cofactor Comment Organism Structure
1.2.1.44 NADP+
-
 Leucaena leucocephala
1.2.1.44 NADPH
-
 Leucaena leucocephala

General Information

EC Number General Information Comment Organism
1.2.1.44 additional information active site characterization of Ll-CCRH1 by modeling/docking, site directed mutagenesis and chemical modification studies, conformational transitions of Ll-CCRH1 are studied using fluorescence and circualar dichroism spectroscopy, overview. Native Ll-CCRH1 is a multi tryptophan protein, the active site of Ll-CCRH1 is made up of 10 residues, that is, Phe30, Ile31, Arg51, Asp77, Ser136, Tyr170, Lys174, Val200, Ser212, and His215 Leucaena leucocephala