BRENDA - Enzyme Database

Functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin

Kallscheuer, N.; Vogt, M.; Bott, M.; Marienhagen, J.; J. Biotechnol. 258, 190-196 (2017)

Data extracted from this reference:

Application
EC Number
Application
Commentary
Organism
1.14.11.9
synthesis
expression of heterologous dioxygenase genes in (2S)-flavanone-producing Corynebacterium glutamicum strains enables the production of flavanonols and flavonols, e.g kaempferol and quercetin, starting from thephenylpropanoids p-coumaric acid and caffeic acid; functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin
Petunia x hybrida
1.14.20.6
synthesis
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin
Populus deltoides
Cloned(Commentary)
EC Number
Cloned (Commentary)
Organism
1.14.11.9
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin; gene F3H, functional recombinant expression of codon-optimized enzyme in Corynebacterium glutamicum strain DelAro4, coexpression with gene FLS encoding flavonol synthase from Populus deltoides. Coexpression fo genes F3H and FLS both from Petunia hybrida is not successful
Petunia x hybrida
1.14.20.6
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin
Populus deltoides
Engineering
EC Number
Protein Variants
Commentary
Organism
1.14.11.9
additional information
the enzyme is ngineered for synthesizing polyphenol core structuressuch as the stilbene resveratrol and the (2S)-flavanone naringenin
Petunia x hybrida
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Petunia x hybrida
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Organism
EC Number
Organism
UniProt
Commentary
Textmining
1.14.11.9
Petunia x hybrida
O22530
-
-
1.14.11.9
Petunia x hybrida
Q07512
-
-
1.14.20.6
Petunia x hybrida
Q07512
-
-
1.14.20.6
Populus deltoides
-
-
-
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
742957
Petunia x hybrida
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
-
742957
Petunia x hybrida
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
742957
Petunia x hybrida
dihydrokaempferol + succinate + CO2
-
-
-
?
Synonyms
EC Number
Synonyms
Commentary
Organism
1.14.11.9
F3H
-
Petunia x hybrida
1.14.11.9
flavanone 3-hydroxylase
-
Petunia x hybrida
Application (protein specific)
EC Number
Application
Commentary
Organism
1.14.11.9
synthesis
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin
Petunia x hybrida
1.14.11.9
synthesis
expression of heterologous dioxygenase genes in (2S)-flavanone-producing Corynebacterium glutamicum strains enables the production of flavanonols and flavonols, e.g kaempferol and quercetin, starting from thephenylpropanoids p-coumaric acid and caffeic acid
Petunia x hybrida
1.14.20.6
synthesis
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin
Populus deltoides
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.14.11.9
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin
Petunia x hybrida
1.14.11.9
gene F3H, functional recombinant expression of codon-optimized enzyme in Corynebacterium glutamicum strain DelAro4, coexpression with gene FLS encoding flavonol synthase from Populus deltoides. Coexpression fo genes F3H and FLS both from Petunia hybrida is not successful
Petunia x hybrida
1.14.20.6
functional expression of plant-derived O-methyltransferase, flavanone 3-hydroxylase, and flavonol synthase in Corynebacterium glutamicum for production of pterostilbene, kaempferol, and quercetin
Populus deltoides
Engineering (protein specific)
EC Number
Protein Variants
Commentary
Organism
1.14.11.9
additional information
the enzyme is ngineered for synthesizing polyphenol core structuressuch as the stilbene resveratrol and the (2S)-flavanone naringenin
Petunia x hybrida
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
Petunia x hybrida
-
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
?
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
1.14.11.9
a (2S)-flavan-4-one + 2-oxoglutarate + O2
-
742957
Petunia x hybrida
a (2R,3R)-dihydroflavonol + succinate + CO2
-
-
-
?
1.14.11.9
eriodictyol + 2-oxoglutarate + O2
-
742957
Petunia x hybrida
dihydroquercetin + succinate + CO2
-
-
-
?
1.14.11.9
naringenin + 2-oxoglutarate + O2
-
742957
Petunia x hybrida
dihydrokaempferol + succinate + CO2
-
-
-
?
General Information
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the family of 2-oxoglutarate-dependent dioxygenases
Petunia x hybrida
1.14.11.9
metabolism
(2S)-flavanones are converted to flavonols by the activity of the 2-oxoglutarate-dependent dioxygenases flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS)
Petunia x hybrida
General Information (protein specific)
EC Number
General Information
Commentary
Organism
1.14.11.9
evolution
the enzyme belongs to the family of 2-oxoglutarate-dependent dioxygenases
Petunia x hybrida
1.14.11.9
metabolism
(2S)-flavanones are converted to flavonols by the activity of the 2-oxoglutarate-dependent dioxygenases flavanone 3-hydroxylase (F3H) and flavonol synthase (FLS)
Petunia x hybrida