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Literature summary extracted from

  • Milhim, M.; Gerber, A.; Neunzig, J.; Hannemann, F.; Bernhardt, R.
    A novel NADPH-dependent flavoprotein reductase from Bacillus megaterium acts as an efficient cytochrome P450 reductase (2016), J. Biotechnol., 231, 83-94 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.6.2.4 expressed in Escherichia coli C43(DE3) cells Priestia megaterium

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.6.2.4 0.0167
-
 ferricytochrome c at pH 7.5 and 25°C Priestia megaterium

Organism

EC Number Organism UniProt Comment Textmining
1.6.2.4 Priestia megaterium
-
-
-
1.6.2.4 Priestia megaterium DSM319
-
-
-

Purification (Commentary)

EC Number Purification (Comment) Organism
1.6.2.4 Ni-NTA column chromatography Priestia megaterium

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.6.2.4 NADPH + H+ + ferricytochrome c
-
 Priestia megaterium NADP+ + ferrocytochrome c
-
 ?
1.6.2.4 NADPH + H+ + ferricytochrome c
-
 Priestia megaterium DSM319 NADP+ + ferrocytochrome c
-
 ?
1.6.2.4 NADPH + H+ + oxidized CYP106A1
-
 Priestia megaterium NADP+ + reduced CYP106A1
-
 ?
1.6.2.4 NADPH + H+ + oxidized CYP106A1
-
 Priestia megaterium DSM319 NADP+ + reduced CYP106A1
-
 ?
1.6.2.4 NADPH + H+ + oxidized CYP21A2
-
 Priestia megaterium NADP+ + reduced CYP21A2
-
 ?
1.6.2.4 NADPH + H+ + oxidized CYP21A2
-
 Priestia megaterium DSM319 NADP+ + reduced CYP21A2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.6.2.4 CPR
-
 Priestia megaterium
1.6.2.4 cytochrome P450 reductase
-
 Priestia megaterium

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.6.2.4 54
-
 the reductase loses 50% of its activity after 10 min at 54°C Priestia megaterium

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.6.2.4 43.03
-
 ferricytochrome c at pH 7.5 and 25°C Priestia megaterium

Cofactor

EC Number Cofactor Comment Organism Structure
1.6.2.4 FAD
-
 Priestia megaterium
1.6.2.4 FMN
-
 Priestia megaterium
1.6.2.4 NADPH
-
 Priestia megaterium