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Literature summary extracted from
- A strategy for soluble overexpression and biochemical characterization of halo-thermotolerant Bacillus laccase in modified E. coli (2016), J. Biotechnol., 227, 56-63 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.10.3.2 | expression of a soluble and functional laccase in modified Escherichia coli, trxB2/gor2 mutant(OrigamiTMB (DE3)) at the expression level suitable for industrial application. The choice of Origami is dictated by the fact that disulfide bridges formation is necessary for properfolding of laccase | Bacillus sp. (in: Bacteria) |
Inhibitors
| EC Number | Inhibitors | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.10.3.2 | dithiothreitol | 0.1 mM, complete inhibition | Bacillus sp. (in: Bacteria) |  |
| 1.10.3.2 | L-cysteine | 0.1 mM, complete inhibition | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | additional information | no inhibition: EDTA (10-50 mM), SDS (0.1-1 mM) | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | NaCl | 1000 mM, 24% inhibition | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | NaN3 | 1 mM, 35% inhibition | Bacillus sp. (in: Bacteria) | |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.2 | 0.0196 | - |
syringaldazine | pH 6.5, 37°C, recombinant enzyme | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | 0.046 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C, recombinant enzyme | Bacillus sp. (in: Bacteria) | |
Organic Solvent Stability
| EC Number | Organic Solvent | Comment | Organism |
|---|---|---|---|
| 1.10.3.2 | acetonitrile | 30% (v/v), 70% loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | dimethyl sulfoxide | 30% (v/v), 65% loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | Ethanol | 30% (v/v), 65% loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | Methanol | 30% (v/v), 50% loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.10.3.2 | Bacillus sp. (in: Bacteria) | A0A165W3F6 | - |
- |
| 1.10.3.2 | Bacillus sp. (in: Bacteria) SL-1 | A0A165W3F6 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.10.3.2 | recombinant enzyme | Bacillus sp. (in: Bacteria) |
Specific Activity [micromol/min/mg]
| EC Number | Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 13 | - |
recombinant enzyme, substrate: syringaldazine, pH 6.5, 37°C, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.10.3.2 | 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) + O2 | - |
Bacillus sp. (in: Bacteria) SL-1 | ? | - |
? | |
| 1.10.3.2 | syringaldazine + O2 | 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) | ? | - |
? | |
| 1.10.3.2 | syringaldazine + O2 | 90% of the activity compared to 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) SL-1 | ? | - |
? | |
Temperature Optimum [°C]
| EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 50 | - |
- |
Bacillus sp. (in: Bacteria) |
Temperature Stability [°C]
| EC Number | Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 70 | - |
half-life: 60 min, recombinant enzyme | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | 80 | - |
half-life: 20 min, recombinant enzyme | Bacillus sp. (in: Bacteria) |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.10.3.2 | 23 | - |
2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | pH 5.0, 25°C, recombinant enzyme | Bacillus sp. (in: Bacteria) | |
| 1.10.3.2 | 24 | - |
syringaldazine | pH 6.5, 37°C, recombinant enzyme | Bacillus sp. (in: Bacteria) | |
pH Optimum
| EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 4.5 | - |
substrate: 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | 6.5 | - |
substrate: syringaldazine | Bacillus sp. (in: Bacteria) |
pH Stability
| EC Number | pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|---|
| 1.10.3.2 | 3.5 | - |
24 h, complete loss of activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | 6.5 | - |
72 h, 22°C, enzyme retains 21% of its activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
| 1.10.3.2 | 8.5 | - |
72 h, 22°C, enzyme retains 17% of its activity, recombinant enzyme | Bacillus sp. (in: Bacteria) |
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Release 2023.1 (January 2023)
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