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Literature summary extracted from

  • Matsubara, T.; Hamada, S.; Wakabayashi, A.; Kishida, M.
    Fermentative production of L-galactonate by using recombinant Saccharomyces cerevisiae containing the endogenous galacturonate reductase gene from Cryptococcus diffluens (2016), J. Biosci. Bioeng., 122, 639-644 .
    View publication on PubMed

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.1.1.365 gene GAR1, DNA and amino acid sequence determination and analysis, functional recombinant expression in Saccharomyces cerevisiae strain IFO 10455 from a genome integrated copy, integration of the pGK406GAR1 plasmid into the ura3 allele of the IFO 10455 genome, subcloning in Escherichia coli strain DH5alpha Naganishia diffluens

Protein Variants

EC Number Protein Variants Comment Organism
1.1.1.365 additional information development of an ethanol fermentation system from D-galacturonic acid (or pectic waste) in enzyme lacking Saccharomyces cerevisiae, brewing yeast. Optimization of conversion of D-galacturonic acid to L-galactonic acid by recombinant enzyme in Saccharomyces cerevisiae, overview. High efficiency in the conversion of D-galacturonic acid to L-galactonic acid in large-scale cultures is achieved with 0.1% initial D-galacturonic acid concentration, pH 3.5, and glucose as additional sugar, aerobic condition is necessary. Subculture of this recombinant is not showing to decrease of the D-galacturonic acid conversion rate even though it is repeated in ten generations. Culturing in scale-up, the conversion rate of D-galacturonic acid to L-galactonic acid is increased. The recombinant strain, similar to its wild-type host strain IFO 10455, cannot grow in media containing D-galacturonic acid as the sole carbon source, but it can grow after the addition of Saccharomyces cerevisiae fermentative sugars like glucose and galactose Naganishia diffluens

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.1.1.365 D-galacturonate + NADPH + H+ Naganishia diffluens
-
 L-galactonate + NADP+
-
 r
1.1.1.365 D-galacturonate + NADPH + H+ Naganishia diffluens OPU-FC11
-
 L-galactonate + NADP+
-
 r

Organism

EC Number Organism UniProt Comment Textmining
1.1.1.365 Naganishia diffluens HV538330 a D-galacturonic acid-utilizing, psychrophilic yeast strain
-
1.1.1.365 Naganishia diffluens OPU-FC11 HV538330 a D-galacturonic acid-utilizing, psychrophilic yeast strain
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.1.1.365 D-galacturonate + NADPH + H+
-
 Naganishia diffluens L-galactonate + NADP+
-
 r
1.1.1.365 D-galacturonate + NADPH + H+
-
 Naganishia diffluens OPU-FC11 L-galactonate + NADP+
-
 r
1.1.1.365 additional information optimization of conversion of D-galacturonic acid to L-galactonic acid by recombinant enzyme in Saccharomyces cerevisiae, overview Naganishia diffluens ?
-
 ?
1.1.1.365 additional information optimization of conversion of D-galacturonic acid to L-galactonic acid by recombinant enzyme in Saccharomyces cerevisiae, overview Naganishia diffluens OPU-FC11 ?
-
 ?

Subunits

EC Number Subunits Comment Organism
1.1.1.365 ? x * 46450, sequence calculation, x * 45000, recombinant enzyme, SDS-PAGE Naganishia diffluens

Synonyms

EC Number Synonyms Comment Organism
1.1.1.365 Cd-GAR
-
 Naganishia diffluens
1.1.1.365 galacturonate reductase
-
 Naganishia diffluens
1.1.1.365 gar1
-
 Naganishia diffluens

pH Optimum

EC Number pH Optimum Minimum pH Optimum Maximum Comment Organism
1.1.1.365 3.5
-
 in vivo activity Naganishia diffluens

Cofactor

EC Number Cofactor Comment Organism Structure
1.1.1.365 NADP+
-
 Naganishia diffluens
1.1.1.365 NADPH
-
 Naganishia diffluens

General Information

EC Number General Information Comment Organism
1.1.1.365 evolution the enzyme belongs to the NAD(P)+-binding Rossmann fold oxidoreductase family of proteins Naganishia diffluens