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Literature summary extracted from

  • Demissie, R.D.; Kabre, P.; Tuntland, M.L.; Fung, L.W.
    An efficient and economical assay to screen for triclosan binding to FabI (2016), J. Biomol. Screen., 21, 391-398 .
    View publication on PubMed

Application

EC Number Application Comment Organism
1.3.1.9 analysis development of a simple thermal shift assay, which does not use ACP-linked substrates, to determine the binding ability of triclosan to the enzyme's active site Francisella tularensis subsp. tularensis
1.3.1.104 analysis development of a simple thermal shift assay, which does not use ACP-linked substrates, to determine the binding ability of triclosan to the enzyme's active site Staphylococcus aureus

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.1.9
-
 Francisella tularensis subsp. tularensis
1.3.1.104
-
 Staphylococcus aureus

Protein Variants

EC Number Protein Variants Comment Organism
1.3.1.9 A92V no shift in melting temperature in presence of both triclosan and NADP+ Francisella tularensis subsp. tularensis
1.3.1.104 A95V about 15% of wild-type activtiy, no inhibition by triclosan and no shift in melting temperature in presence of both triclosan and NADP+ Staphylococcus aureus
1.3.1.104 F204S no shift in melting temperature in presence of both triclosan and NADP+ Staphylococcus aureus
1.3.1.104 I193S no shift in melting temperature in presence of both triclosan and NADP+ Staphylococcus aureus
1.3.1.104 M99T mutant shows a similar shift in melting temperature in presence of both triclosan and NADP+ as wild-type Staphylococcus aureus

Inhibitors

EC Number Inhibitors Comment Organism Structure
1.3.1.9 triclosan
-
 Francisella tularensis subsp. tularensis
1.3.1.104 triclosan 1.12 microM, 9% residual activity Staphylococcus aureus

Organism

EC Number Organism UniProt Comment Textmining
1.3.1.9 Francisella tularensis subsp. tularensis Q5NGQ3
-
-
1.3.1.104 Staphylococcus aureus A0A0H3K7V7
-
-
1.3.1.104 Staphylococcus aureus Newman A0A0H3K7V7
-
-

Specific Activity [micromol/min/mg]

EC Number Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
1.3.1.104 0.66
-
 pH not specified in the publication, 25°C Staphylococcus aureus

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.1.9 crotonyl-CoA + NADH + H+
-
 Francisella tularensis subsp. tularensis butyryl-CoA + NAD+
-
 ?
1.3.1.104 crotonyl-[acyl-carrier-protein] + NADPH + H+
-
 Staphylococcus aureus butyryl-[acyl-carrier-protein] + NADP+
-
 ?
1.3.1.104 crotonyl-[acyl-carrier-protein] + NADPH + H+
-
 Staphylococcus aureus Newman butyryl-[acyl-carrier-protein] + NADP+
-
 ?

Subunits

EC Number Subunits Comment Organism
1.3.1.104 ? x * 30397, calculated, x * 30397, mass spectrometry Staphylococcus aureus

Synonyms

EC Number Synonyms Comment Organism
1.3.1.9 FabI
-
 Francisella tularensis subsp. tularensis
1.3.1.104 FabI
-
 Staphylococcus aureus
1.3.1.104 NWMN_0881
-
 Staphylococcus aureus

Temperature Stability [°C]

EC Number Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
1.3.1.9 53.2
-
 wild-type, melting temperature in presence of both triclosan and NADP+. Neither triclosan nor NADP+ alone increases the Tm value Francisella tularensis subsp. tularensis
1.3.1.9 60.2
-
 wild-type, melting temperature Francisella tularensis subsp. tularensis
1.3.1.104 39.4
-
 mutant A95V, melting temperature Staphylococcus aureus
1.3.1.104 40.2
-
 mutant M99T, melting temperature Staphylococcus aureus
1.3.1.104 40.5
-
 wild-type, melting temperature Staphylococcus aureus
1.3.1.104 58.8
-
 wild-type, melting temperature in presence of both triclosan and NADP+. Neither triclosan nor NADP+ alone increases the Tm value Staphylococcus aureus
1.3.1.104 60.2
-
 mutant M99T, melting temperature in presence of both triclosan and NADP+ Staphylococcus aureus