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Literature summary extracted from

  • Juillan-Binard, C.; Picciocchi, A.; Andrieu, J.P.; Dupuy, J.; Petit-Hartlein, I.; Caux-Thang, C.; Vives, C.; Niviere, V.; Fieschi, F.
    A two-component NADPH oxidase (NOX)-like system in bacteria is involved in the electron transfer chain to the methionine sulfoxide reductase MsrP (2017), J. Biol. Chem., 292, 2485-2494 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.8.5.B1 expression in Escherichia coli Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.8.5.B1 H151A mutant has aout 57% of the heme content of wild-type Escherichia coli
1.8.5.B1 H164A mutant has aout 63% of the heme content of wild-type Escherichia coli
1.8.5.B1 H91A almost complete loss of heme binding Escherichia coli

Localization

EC Number Localization Comment Organism GeneOntology No. Textmining
1.8.5.B1 membrane subunit MsrQ Escherichia coli 16020
-

Organism

EC Number Organism UniProt Comment Textmining
1.8.5.B1 Escherichia coli P76342 and P76343 P76342 i.e. catalytic subunit MsrP, P76343 i.e. heme-binding subunit MsrQ
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.8.5.B1 additional information subunit MsrQ is reduced due to the free reduced FMN generated by the flavin reductase Fre activity Escherichia coli ?
-
 ?
1.8.5.B1 [protein]-L-methionine + a quinone + H2O
-
 Escherichia coli [protein]-L-methionine (R)-S-oxide + a quinol
-
 r
1.8.5.B1 [protein]-L-methionine + a quinone + H2O
-
 Escherichia coli [protein]-L-methionine (S)-S-oxide + a quinol
-
 r

Subunits

EC Number Subunits Comment Organism
1.8.5.B1 More protein physically interacts with flavin reductase Fre Escherichia coli

Synonyms

EC Number Synonyms Comment Organism
1.8.5.B1 MsrP
-
 Escherichia coli
1.8.5.B1 MsrQ
-
 Escherichia coli
1.8.5.B1 protein-methionine-sulfoxide reductase
-
 Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.8.5.B1 heme heme/protein ratio is 2.5. The extinction coefficient value for the oxidized subunit MsrQ form at 412 nm is 51950 per M and cm. For the reduced MsrQ form at 426 and 558 nm, the extinction coefficient values are 69015 per M and cm and 11075 per M and cm, respectively Escherichia coli