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Literature summary extracted from
- H2S oxidation by nanodisc-embedded human sulfide quinone oxidoreductase (2017), J. Biol. Chem., 292, 11641-11649 .
Cloned(Commentary)
| EC Number | Cloned (Comment) | Organism |
|---|---|---|
| 1.8.5.4 | expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
| 1.8.5.8 | expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
KM Value [mM]
| EC Number | KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.4 | 0.23 | - |
Sulfide | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens |  |
| 1.8.5.4 | 0.35 | - |
Sulfide | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 0.008 | - |
glutathione | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 0.008 | - |
glutathione | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 0.19 | - |
sulfite | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 0.23 | - |
Sulfide | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 0.26 | - |
sulfite | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 0.35 | - |
Sulfide | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
Natural Substrates/ Products (Substrates)
| EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.4 | sulfide + glutathione | Homo sapiens | - |
sulfur + reduced glutathione | - |
? | |
| 1.8.5.8 | hydrogen sulfide + glutathione + a quinone | Homo sapiens | - |
S-sulfanylglutathione + a quinol | - |
? | |
Organism
| EC Number | Organism | UniProt | Comment | Textmining |
|---|---|---|---|---|
| 1.8.5.4 | Homo sapiens | Q9Y6N5 | - |
- |
| 1.8.5.8 | Homo sapiens | Q9Y6N5 | - |
- |
Purification (Commentary)
| EC Number | Purification (Comment) | Organism |
|---|---|---|
| 1.8.5.4 | nickel affinity column chromatography and Superdex 200 gel filtration | Homo sapiens |
Substrates and Products (Substrate)
| EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|---|
| 1.8.5.4 | sulfide + coenzyme Q1 | - |
Homo sapiens | sulfur + reduced coenzyme Q1 | - |
? | |
| 1.8.5.4 | sulfide + coenzyme Q10 | - |
Homo sapiens | sulfur + reduced coenzyme Q10 | - |
? | |
| 1.8.5.4 | sulfide + glutathione | - |
Homo sapiens | sulfur + reduced glutathione | - |
? | |
| 1.8.5.8 | hydrogen sulfide + glutathione + a quinone | - |
Homo sapiens | S-sulfanylglutathione + a quinol | - |
? | |
| 1.8.5.8 | hydrogen sulfide + glutathione + coenzyme Q | - |
Homo sapiens | S-sulfanylglutathione + reduced coenzyme Q | - |
? | |
| 1.8.5.8 | hydrogen sulfide + sulfide + coenzyme Q | - |
Homo sapiens | ? | - |
? | |
| 1.8.5.8 | hydrogen sulfide + sulfite + coenzyme Q | - |
Homo sapiens | ? | - |
? | |
Subunits
| EC Number | Subunits | Comment | Organism |
|---|---|---|---|
| 1.8.5.4 | ? | x * 50000, SDS-PAGE | Homo sapiens |
| 1.8.5.8 | ? | x * 50000, SDS-PAGE | Homo sapiens |
Synonyms
| EC Number | Synonyms | Comment | Organism |
|---|---|---|---|
| 1.8.5.4 | SQR | - |
Homo sapiens |
| 1.8.5.4 | sulfide quinone oxidoreductase | - |
Homo sapiens |
| 1.8.5.8 | SQR | - |
Homo sapiens |
| 1.8.5.8 | sulfide quinone oxidoreductase | - |
Homo sapiens |
Turnover Number [1/s]
| EC Number | Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.4 | 62 | - |
Sulfide | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.4 | 84 | - |
Sulfide | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 62 | - |
Sulfide | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 84 | - |
Sulfide | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 89 | - |
glutathione | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 128 | - |
glutathione | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 382 | - |
sulfite | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 650 | - |
sulfite | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
Cofactor
| EC Number | Cofactor | Comment | Organism | Structure |
|---|---|---|---|---|
| 1.8.5.4 | FAD | - |
Homo sapiens | |
| 1.8.5.8 | FAD | - |
Homo sapiens | |
kcat/KM [mM/s]
| EC Number | kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|---|
| 1.8.5.4 | 180 | - |
Sulfide | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.4 | 370 | - |
Sulfide | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 11 | - |
glutathione | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 16 | - |
glutathione | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 180 | - |
Sulfide | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 370 | - |
Sulfide | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 2000 | - |
sulfite | solubilized enzyme, at pH 6.8 and 25°C | Homo sapiens | |
| 1.8.5.8 | 2500 | - |
sulfite | enzyme in nanodiscs, at pH 6.8 and 25°C | Homo sapiens | |
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Release 2023.1 (January 2023)
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