BRENDA - Enzyme Database

H2S oxidation by nanodisc-embedded human sulfide quinone oxidoreductase

Landry, A.; Ballou, D.; Banerjee, R.; J. Biol. Chem. 292, 11641-11649 (2017)

Data extracted from this reference:

Cloned(Commentary)
EC Number
Commentary
Organism
1.8.5.4
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
1.8.5.8
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
KM Value [mM]
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.5.4
0.23
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.4
0.35
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.008
-
glutathione
enzyme in nanodiscs, at pH 6.8 and 25C; solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.19
-
sulfite
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.23
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.26
-
sulfite
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.35
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
Natural Substrates/ Products (Substrates)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.5.4
sulfide + glutathione
Homo sapiens
-
sulfur + reduced glutathione
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + a quinone
Homo sapiens
-
S-sulfanylglutathione + a quinol
-
-
?
Organism
EC Number
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
1.8.5.4
Homo sapiens
Q9Y6N5
-
-
1.8.5.8
Homo sapiens
Q9Y6N5
-
-
Purification (Commentary)
EC Number
Commentary
Organism
1.8.5.4
nickel affinity column chromatography and Superdex 200 gel filtration
Homo sapiens
Substrates and Products (Substrate)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.5.4
sulfide + coenzyme Q1
-
742905
Homo sapiens
sulfur + reduced coenzyme Q1
-
-
-
?
1.8.5.4
sulfide + coenzyme Q10
-
742905
Homo sapiens
sulfur + reduced coenzyme Q10
-
-
-
?
1.8.5.4
sulfide + glutathione
-
742905
Homo sapiens
sulfur + reduced glutathione
-
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + a quinone
-
742905
Homo sapiens
S-sulfanylglutathione + a quinol
-
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + coenzyme Q
-
742905
Homo sapiens
S-sulfanylglutathione + reduced coenzyme Q
-
-
-
?
1.8.5.8
hydrogen sulfide + sulfide + coenzyme Q
-
742905
Homo sapiens
?
-
-
-
?
1.8.5.8
hydrogen sulfide + sulfite + coenzyme Q
-
742905
Homo sapiens
?
-
-
-
?
Subunits
EC Number
Subunits
Commentary
Organism
1.8.5.4
?
x * 50000, SDS-PAGE
Homo sapiens
1.8.5.8
?
x * 50000, SDS-PAGE
Homo sapiens
Turnover Number [1/s]
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.5.4
62
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.4
84
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
62
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
84
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
89
-
glutathione
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
128
-
glutathione
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
382
-
sulfite
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
650
-
sulfite
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
Cofactor
EC Number
Cofactor
Commentary
Organism
Structure
1.8.5.4
FAD
-
Homo sapiens
1.8.5.8
FAD
-
Homo sapiens
Cloned(Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.5.4
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
1.8.5.8
expressed in Escherichia coli BL21(DE3) cells
Homo sapiens
Cofactor (protein specific)
EC Number
Cofactor
Commentary
Organism
Structure
1.8.5.4
FAD
-
Homo sapiens
1.8.5.8
FAD
-
Homo sapiens
KM Value [mM] (protein specific)
EC Number
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
1.8.5.4
0.23
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.4
0.35
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.008
-
glutathione
enzyme in nanodiscs, at pH 6.8 and 25C; solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.19
-
sulfite
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.23
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.26
-
sulfite
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
0.35
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
EC Number
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
1.8.5.4
sulfide + glutathione
Homo sapiens
-
sulfur + reduced glutathione
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + a quinone
Homo sapiens
-
S-sulfanylglutathione + a quinol
-
-
?
Purification (Commentary) (protein specific)
EC Number
Commentary
Organism
1.8.5.4
nickel affinity column chromatography and Superdex 200 gel filtration
Homo sapiens
Substrates and Products (Substrate) (protein specific)
EC Number
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
1.8.5.4
sulfide + coenzyme Q1
-
742905
Homo sapiens
sulfur + reduced coenzyme Q1
-
-
-
?
1.8.5.4
sulfide + coenzyme Q10
-
742905
Homo sapiens
sulfur + reduced coenzyme Q10
-
-
-
?
1.8.5.4
sulfide + glutathione
-
742905
Homo sapiens
sulfur + reduced glutathione
-
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + a quinone
-
742905
Homo sapiens
S-sulfanylglutathione + a quinol
-
-
-
?
1.8.5.8
hydrogen sulfide + glutathione + coenzyme Q
-
742905
Homo sapiens
S-sulfanylglutathione + reduced coenzyme Q
-
-
-
?
1.8.5.8
hydrogen sulfide + sulfide + coenzyme Q
-
742905
Homo sapiens
?
-
-
-
?
1.8.5.8
hydrogen sulfide + sulfite + coenzyme Q
-
742905
Homo sapiens
?
-
-
-
?
Subunits (protein specific)
EC Number
Subunits
Commentary
Organism
1.8.5.4
?
x * 50000, SDS-PAGE
Homo sapiens
1.8.5.8
?
x * 50000, SDS-PAGE
Homo sapiens
Turnover Number [1/s] (protein specific)
EC Number
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
1.8.5.4
62
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.4
84
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
62
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
84
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
89
-
glutathione
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
128
-
glutathione
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
382
-
sulfite
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
650
-
sulfite
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
KCat/KM [mM/s]
EC Number
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.8.5.4
180
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.4
370
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
11
-
glutathione
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
16
-
glutathione
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
180
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
370
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
2000
-
sulfite
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
2500
-
sulfite
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
KCat/KM [mM/s] (protein specific)
EC Number
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
1.8.5.4
180
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.4
370
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
11
-
glutathione
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
16
-
glutathione
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
180
-
Sulfide
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
370
-
Sulfide
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
2000
-
sulfite
solubilized enzyme, at pH 6.8 and 25C
Homo sapiens
1.8.5.8
2500
-
sulfite
enzyme in nanodiscs, at pH 6.8 and 25C
Homo sapiens