EC Number | Cloned (Comment) | Organism |
---|---|---|
1.5.5.1 | cloning of a putative ETF-QO orthologue, DNA and amino acid sequence determination and analysis, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus(DE3)-RIPL | Agrobacterium tumefaciens |
1.5.5.1 | recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21 CodonPlus(DE3)-RIPL | Homo sapiens |
EC Number | Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
EC Number | Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.5.1 | reduced electron-transferring flavoprotein + ubiquinone | Homo sapiens | - |
electron-transferring flavoprotein + ubiquinol | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + ubiquinone | Agrobacterium tumefaciens | - |
electron-transferring flavoprotein + ubiquinol | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + ubiquinone | Agrobacterium tumefaciens C58 / ATCC 33970 | - |
electron-transferring flavoprotein + ubiquinol | - |
? |
EC Number | Organism | UniProt | Comment | Textmining |
---|---|---|---|---|
1.5.5.1 | Agrobacterium tumefaciens | A9CJL8 | - |
- |
1.5.5.1 | Agrobacterium tumefaciens C58 / ATCC 33970 | A9CJL8 | - |
- |
1.5.5.1 | Homo sapiens | Q16134 | - |
- |
EC Number | Purification (Comment) | Organism |
---|---|---|
1.5.5.1 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus(DE3)-RIPL by nickel affinity chromatography in presence of FAD | Homo sapiens |
1.5.5.1 | recombinant N-terminally His6-tagged enzyme from Escherichia coli strain BL21 CodonPlus(DE3)-RIPL by nickel affinity chromatography in presence of FAD | Agrobacterium tumefaciens |
EC Number | Source Tissue | Comment | Organism | Textmining |
---|---|---|---|---|
1.5.5.1 | HeLa cell | - |
Homo sapiens | - |
EC Number | Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|---|
1.5.5.1 | additional information | although Agrobacterium tumefaciens ETF-mediated electron transfer to 2,6-DCIP is observed with a number of different dehydrogenases, the reduction of coenzyme Q1 through the DH/ETF/ETF-QO system is only observed with dimethylglycine dehydrogenase, but not with any of the tested Agrobacterium tumefaciens acyl-CoA dehydrogenases | Agrobacterium tumefaciens | ? | - |
? | |
1.5.5.1 | additional information | the recombinant human enzyme is able to transfer electrons from the ETF protein from Agrobacterium tumefaciens strain C58 to 2,6-dichloroindophenol or coenzyme Q1 in an in vitro system | Homo sapiens | ? | - |
? | |
1.5.5.1 | additional information | although Agrobacterium tumefaciens ETF-mediated electron transfer to 2,6-DCIP is observed with a number of different dehydrogenases, the reduction of coenzyme Q1 through the DH/ETF/ETF-QO system is only observed with dimethylglycine dehydrogenase, but not with any of the tested Agrobacterium tumefaciens acyl-CoA dehydrogenases | Agrobacterium tumefaciens C58 / ATCC 33970 | ? | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + 2,6-dichloroindophenol | - |
Homo sapiens | electron-transferring flavoprotein + reduced 2,6-dichloroindophenol | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + 2,6-dichloroindophenol | - |
Agrobacterium tumefaciens | electron-transferring flavoprotein + reduced 2,6-dichloroindophenol | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + 2,6-dichloroindophenol | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | electron-transferring flavoprotein + reduced 2,6-dichloroindophenol | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + coenzyme Q1 | - |
Homo sapiens | electron-transferring flavoprotein + reduced coenzyme Q1 | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + coenzyme Q1 | - |
Agrobacterium tumefaciens | electron-transferring flavoprotein + reduced coenzyme Q1 | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + coenzyme Q1 | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | electron-transferring flavoprotein + reduced coenzyme Q1 | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + ubiquinone | - |
Homo sapiens | electron-transferring flavoprotein + ubiquinol | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + ubiquinone | - |
Agrobacterium tumefaciens | electron-transferring flavoprotein + ubiquinol | - |
? | |
1.5.5.1 | reduced electron-transferring flavoprotein + ubiquinone | - |
Agrobacterium tumefaciens C58 / ATCC 33970 | electron-transferring flavoprotein + ubiquinol | - |
? |
EC Number | Synonyms | Comment | Organism |
---|---|---|---|
1.5.5.1 | ETF-QO | - |
Homo sapiens |
1.5.5.1 | ETF-QO | - |
Agrobacterium tumefaciens |
1.5.5.1 | ETF:quinone oxidoreductase | - |
Homo sapiens |
1.5.5.1 | ETF:quinone oxidoreductase | - |
Agrobacterium tumefaciens |
EC Number | Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|---|
1.5.5.1 | 22 | - |
assay at room temperature | Homo sapiens |
1.5.5.1 | 22 | - |
assay at room temperature | Agrobacterium tumefaciens |
EC Number | pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|---|
1.5.5.1 | 7.2 | - |
assay at | Homo sapiens |
1.5.5.1 | 7.2 | - |
assay at | Agrobacterium tumefaciens |
EC Number | Cofactor | Comment | Organism | Structure |
---|---|---|---|---|
1.5.5.1 | FAD | - |
Homo sapiens | |
1.5.5.1 | FAD | - |
Agrobacterium tumefaciens | |
1.5.5.1 | ubiquinone | - |
Homo sapiens | |
1.5.5.1 | ubiquinone | - |
Agrobacterium tumefaciens | |
1.5.5.1 | [4Fe-4S]-center | - |
Homo sapiens | |
1.5.5.1 | [4Fe-4S]-center | - |
Agrobacterium tumefaciens |
EC Number | General Information | Comment | Organism |
---|---|---|---|
1.5.5.1 | malfunction | impairment of lysine-specific reduction of ETF reduces the ability of AtETF to mediate electron transfer between ETF-dependent dehydrogenases and ETF-QO | Agrobacterium tumefaciens |
1.5.5.1 | physiological function | electron-transferring flavoprotein, ETF, mediates transfer of electrons from several mitochondrial FAD-containing dehydrogenases to the ETF:quinone oxidoreductase, ETF-QO, leading to reduction of the quinone pool of the mitochondrial respiratory chain | Homo sapiens |
1.5.5.1 | physiological function | electron-transferring flavoprotein, ETF, mediates transfer of electrons from several mitochondrial FAD-containing dehydrogenases to the ETF:quinone oxidoreductase, ETF-QO, leading to reduction of the quinone pool of the mitochondrial respiratory chain | Agrobacterium tumefaciens |