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Literature summary extracted from

  • Sehanobish, E.; Williamson, H.R.; Davidson, V.L.
    Roles of conserved residues of the glycine oxidase GoxA in controlling activity, cooperativity, subunit composition, and cysteine tryptophylquinone biosynthesis (2016), J. Biol. Chem., 291, 23199-23207 .
    View publication on PubMedView publication on EuropePMC

Protein Variants

EC Number Protein Variants Comment Organism
1.4.3.19 F237A the mutant shows stronlgy reduced catalytic efficiency compared to the wild type enzyme Marinomonas mediterranea
1.4.3.19 F237Y the mutant shows reduced catalytic efficiency compared to the wild type enzyme Marinomonas mediterranea

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.4.3.19 0.416
-
 O2 mutant enzyme F237A, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 0.826
-
 glycine mutant enzyme F237A, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 2.27
-
 O2 mutant enzyme F237Y, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 2.6
-
 O2 wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 3.03
-
 glycine mutant enzyme F237Y, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 39
-
 glycine wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea

Natural Substrates/ Products (Substrates)

EC Number Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
1.4.3.19 2-iminoacetate + H2O Marinomonas mediterranea
-
 glyoxylate + NH3
-
 ?
1.4.3.19 glycine + H2O + O2 Marinomonas mediterranea
-
 glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + O2 Marinomonas mediterranea
-
 2-iminoacetate + H2O2
-
 ?

Organism

EC Number Organism UniProt Comment Textmining
1.4.3.19 Marinomonas mediterranea
-
-
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.4.3.19 2-iminoacetate + H2O
-
 Marinomonas mediterranea glyoxylate + NH3
-
 ?
1.4.3.19 glycine + H2O + O2
-
 Marinomonas mediterranea glyoxylate + NH3 + H2O2
-
 ?
1.4.3.19 glycine + O2
-
 Marinomonas mediterranea 2-iminoacetate + H2O2
-
 ?

Synonyms

EC Number Synonyms Comment Organism
1.4.3.19 GoxA
-
 Marinomonas mediterranea

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.4.3.19 0.6
-
 O2 mutant enzyme F237A, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 15
-
 O2 mutant enzyme F237Y, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 39
-
 glycine wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 93
-
 O2 wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea

kcat/KM [mM/s]

EC Number kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
1.4.3.19 1
-
 O2 mutant enzyme F237A, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 6.6
-
 O2 mutant enzyme F237Y, at 30°C, pH not specified in the publication Marinomonas mediterranea
1.4.3.19 36
-
 O2 wild type enzyme, at 30°C, pH not specified in the publication Marinomonas mediterranea