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Literature summary extracted from

  • Singh, P.K.; Sarwar, M.; Maklashina, E.; Kotlyar, V.; Rajagukguk, S.; Tomasiak, T.M.; Cecchini, G.; Iverson, T.M.
    Plasticity of the quinone-binding site of the complex II homolog quinol fumarate reductase (2013), J. Biol. Chem., 288, 24293-24301 .
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

EC Number Cloned (Comment) Organism
1.3.5.1
-
Escherichia coli

Crystallization (Commentary)

EC Number Crystallization (Comment) Organism
1.3.5.1 subunit FrdC mutant E29L, to 2.95 A resolution. The sequential removal of the two menaquinol protons may be accompanied by a rotation of the naphthoquinone ring to optimize the interaction with a second proton shuttling pathway Escherichia coli

Protein Variants

EC Number Protein Variants Comment Organism
1.3.5.1 E29L mutation in subunit FrdC, alters hydrogen bonding to menaquinone Escherichia coli
1.3.5.1 R28E/E29I mutation in subunit FrdC, retains normal level of activity Escherichia coli
1.3.5.1 R28L mutation in subunit FrdC, retains normal level of activity Escherichia coli
1.3.5.1 R28L/E9L mutation in subunit FrdC, retains normal level of activity Escherichia coli
1.3.5.1 R28L/R81A mutation in subunits FrdC/FrdD, loss of activtiy with ubiquinone and menaquinol Escherichia coli
1.3.5.1 R28N mutation in subunit FrdC, retains normal level of activity Escherichia coli
1.3.5.1 R81A mutation in subunit FrdD, retains normal level of activity Escherichia coli
1.3.5.1 R81E mutation in subunit FrdD, retains normal level of activity Escherichia coli
1.3.5.1 R81K mutation in subunit FrdD, retains normal level of activity Escherichia coli

KM Value [mM]

EC Number KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
1.3.5.1 0.0005
-
ubiquinone mutant R81E, pH 8.0, 30°C Escherichia coli
1.3.5.1 0.0006
-
ubiquinone mutant R81A, pH 8.0, 30°C Escherichia coli
1.3.5.1 0.0009
-
menaquinol mutant R81A, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.0013
-
menaquinol mutant R81E, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.0013
-
ubiquinone mutant R81K, pH 8.0, 30°C Escherichia coli
1.3.5.1 0.0015
-
ubiquinone mutant R28L, pH 8.0, 30°C Escherichia coli
1.3.5.1 0.0015
-
ubiquinone mutant R28N, pH 8.0, 30°C Escherichia coli
1.3.5.1 0.002
-
ubiquinone mutant R28L/E29L, pH 8.0, 30°C Escherichia coli
1.3.5.1 0.0023
-
ubiquinone wild-type, pH 8.0, 30°C Escherichia coli
1.3.5.1 0.0027
-
menaquinol mutant R81K, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.003
-
menaquinol mutant R28L, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.0031
-
menaquinol mutant R28N, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.0038
-
ubiquinone mutant R28E/E29I, pH 8.0, 30°C Escherichia coli
1.3.5.1 0.0039
-
menaquinol mutant R28L/E29L, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.004
-
menaquinol mutant R28E/E29I, pH 7.0, 30°C Escherichia coli
1.3.5.1 0.0054
-
menaquinol wild-type, pH 7.0, 30°C Escherichia coli

Organism

EC Number Organism UniProt Comment Textmining
1.3.5.1 Escherichia coli P00363 and P0A8Q0 and P0A8Q3 P00363 i.e. subunit FrdA, P0A8Q0 i.e. subunit FrdC, P0A8Q3 i.e. subunit FrdD
-

Substrates and Products (Substrate)

EC Number Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1.3.5.1 fumarate + menaquinol
-
Escherichia coli succinate + menaquinone
-
?
1.3.5.1 succinate + ferricyanide
-
Escherichia coli fumarate + ferrocyanide
-
?
1.3.5.1 succinate + ubiquinone
-
Escherichia coli fumarate + ubiquinol
-
?

Synonyms

EC Number Synonyms Comment Organism
1.3.5.1 FrdA flavoprotein subunit Escherichia coli
1.3.5.1 FrdC
-
Escherichia coli
1.3.5.1 FrdD
-
Escherichia coli

Turnover Number [1/s]

EC Number Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
1.3.5.1 1.7
-
ubiquinone mutant R28E/E29I, pH 8.0, 30°C Escherichia coli
1.3.5.1 2.8
-
menaquinol mutant R28E/E29I, pH 8.0, 30°C Escherichia coli
1.3.5.1 3
-
menaquinol mutant R28E/E29I, pH 7.0, 30°C Escherichia coli
1.3.5.1 6.1
-
menaquinol mutant R81A, pH 8.0, 30°C Escherichia coli
1.3.5.1 10.6
-
menaquinol mutant R81A, pH 7.0, 30°C Escherichia coli
1.3.5.1 10.7
-
ubiquinone mutant R81E, pH 8.0, 30°C Escherichia coli
1.3.5.1 11.1
-
menaquinol mutant R28L/E29L, pH 8.0, 30°C Escherichia coli
1.3.5.1 12.2
-
menaquinol mutant R28L/E29L, pH 7.0, 30°C Escherichia coli
1.3.5.1 12.8
-
menaquinol mutant R81E, pH 8.0, 30°C Escherichia coli
1.3.5.1 13.9
-
ubiquinone mutant R28L/E29L, pH 8.0, 30°C Escherichia coli
1.3.5.1 14
-
menaquinol mutant R81E, pH 7.0, 30°C Escherichia coli
1.3.5.1 17.4
-
ubiquinone mutant R81A, pH 8.0, 30°C Escherichia coli
1.3.5.1 19
-
ubiquinone mutant R28N, pH 8.0, 30°C Escherichia coli
1.3.5.1 19.3
-
ubiquinone mutant R28L, pH 8.0, 30°C Escherichia coli
1.3.5.1 20.1
-
ferricyanide mutant R28L/R81A, pH 8.0, 30°C Escherichia coli
1.3.5.1 24.6
-
ferricyanide mutant R28N, pH 8.0, 30°C Escherichia coli
1.3.5.1 24.9
-
ubiquinone wild-type, pH 8.0, 30°C Escherichia coli
1.3.5.1 26.4
-
ferricyanide mutant R28E/E29I, pH 8.0, 30°C Escherichia coli
1.3.5.1 27.1
-
ferricyanide mutant R28L, pH 8.0, 30°C Escherichia coli
1.3.5.1 27.3
-
ferricyanide mutant R81E, pH 8.0, 30°C Escherichia coli
1.3.5.1 28.1
-
ferricyanide mutant R81A, pH 8.0, 30°C Escherichia coli
1.3.5.1 28.7
-
ferricyanide wild-type, pH 8.0, 30°C Escherichia coli
1.3.5.1 28.9
-
ferricyanide mutant R28L/E29L, pH 8.0, 30°C Escherichia coli
1.3.5.1 30.4
-
ferricyanide mutant R81K, pH 8.0, 30°C Escherichia coli
1.3.5.1 32.2
-
ubiquinone mutant R81K, pH 8.0, 30°C Escherichia coli
1.3.5.1 65.4
-
menaquinol mutant R28N, pH 8.0, 30°C Escherichia coli
1.3.5.1 67.8
-
menaquinol mutant R28L, pH 8.0, 30°C Escherichia coli
1.3.5.1 68.6
-
menaquinol mutant R28N, pH 7.0, 30°C Escherichia coli
1.3.5.1 71.1
-
menaquinol mutant R28L, pH 7.0, 30°C Escherichia coli
1.3.5.1 123.7
-
menaquinol mutant R81K, pH 7.0, 30°C Escherichia coli
1.3.5.1 138.9
-
menaquinol mutant R81K, pH 8.0, 30°C Escherichia coli
1.3.5.1 211
-
menaquinol wild-type, pH 8.0, 30°C Escherichia coli
1.3.5.1 222.2
-
menaquinol wild-type, pH 7.0, 30°C Escherichia coli

Cofactor

EC Number Cofactor Comment Organism Structure
1.3.5.1 FAD
-
Escherichia coli
1.3.5.1 [3Fe-4S]-center located in subunit FrdC Escherichia coli